Anti-Adipo，脂联素受体-1抗体

• Product nameAnti-Adiponectin antibody [19F1]See all Adiponectin primary antibodies ...
• Description
  Mouse monoclonal [19F1] to Adiponectin
• Tested applicationsELISA, ICC/IF, ICC, IHC-P, WB more details
• Species reactivity
  Reacts with: Mouse, Human, Baboon
• Immunogen

  Recombinant full length protein （Human）.

• Positive control3T3-L1 adipocytes

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot ａnd store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: PBS, 1mg/ml BSA
• Concentration100 µg at 1 mg/ml
  
• PurityProtein G purified
• ClonalityMonoclonal
• Clone number19F1
• IsotypeIgG
• Research Areas
  • Cardiovascular
  • Lipids / Lipoproteins
  • Adipose Related
  • Acrp

  • Signal Transduction
  • Growth Factors/Hormones
  • Hormones

  • Neuroscience
  • Neurology process
  • Metabolism

  • Stem Cells
  • Mesenchymal Stem Cells
  • Adipogenesis

  • Cardiovascular
  • Atherosclerosis
  • Diabetes associated

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Metabolism
  • Pathways ａnd Processes
  • Metabolism processes
  • Hypoxia

  • Metabolism
  • Types of disease
  • Diabetes

  • Metabolism
  • Types of disease
  • Obesity

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Types of disease
  • Metabolic disorders

Applications

• Application notesELISA: Use at an assay dependent dilution.
  ICC: Use at an assay dependent dilution （PMID 19657392）.
  IF: Use at a concentration of 2 - 4 µg/ml.
  IHC-P: Use at an assay dependent concentration.
  WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 30 kDa （predicted molecular weight: 26 kDa）.
  
  
  Not yet tested in other applications.
  Optimal dilutions/concentrations should be determined by the end user.

Target

• FunctionImportant adipokine involved in the control of fat metabolism ａnd insulin sensitivity, with direct anti-diabetic, anti-atherogenic ａnd anti-inflammatory activities. Stimulates AMPK phosphorylation ａnd activation in the liver ａnd the skeletal muscle, enhancing glucose utilization ａnd fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver ａnd macrophages, ａnd also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis ａnd tissue remodeling by binding ａnd sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
• Tissue specificitySynthesized exclusively by adipocytes ａnd secreted into plasma.
• Involvement in diseaseDefects in ADIPOQ are the cause of adiponectin deficiency （ADPND） [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
  Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus （NIDDM） [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood ａnd insulin resistance.
• Sequence similaritiesContains 1 C1q domain.
  Contains 1 collagen-like domain.
• DomainThe C1q domain is commonly called the globular domain.
• Post-translational
  modificationsHydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
  HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation ａnd glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes ａnd consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
  O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
• Cellular localizationSecreted.
• Information by UniProt
• Database links
  • Entrez Gene: 9370 Human
  • Entrez Gene: 11450 Mouse
  • Omim: 605441 Human
  • SwissProt: Q15848 Human
  • SwissProt: Q60994 Mouse
  • Unigene: 80485 Human
  • Unigene: 3969 Mouse
• Alternative names
  • 30 kDa adipocyte complement related protein antibody
  • 30 kDa adipocyte complement-related protein antibody
  • ACDC antibody

  see all

Anti-Adiponectin antibody [19F1] images

• 

  Western blot - Adiponectin antibody [19F1] （ab22554）
  Anti-Adiponectin antibody [19F1] （ab22554） at 1 µg/ml +3T3-L1 （Mouse embryonic fibroblast - adipose like cell line） Nuclear Lysate （ab14632）
  
  Predicted band size : 26 kDa
  Observed band size : 30 kDa （why is the actual band size different from the predicted?）
  
• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Adiponectin antibody [19F1] （ab22554）This image is courtesy of an anonymous Abreview
  ab22554 staining Adiponectin in Mouse skin tissue sections by Immunohistochemistry （IHC-P - paraformaldehyde-fixed, paraffin-embedded sections）. Tissue was fixed with paraformaldehyde ａnd blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation with a citrate buffer （pH6）. Samples were incubated with primary antibody （1/100） for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse polyclonal （1/200） was used as the secondary antibody.

  See Abreview

• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections）-Adiponectin antibody [19F1]（ab22554）
  ab22554 （4µg/ml） staining adiponectin in human breast, using an automated system （DAKO Autostainer Plus）. Using this protocol there is nuclear ａnd cytoplasmic staining.
  Sections were rehydrated ａnd antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes （containing casein 0.25% in PBS） then incubated with primary antibody for 20 min ａnd detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin ａnd coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration ａnd incubation time is recommended. Signal amplification may be required.
  
• 

  Immunocytochemistry/ Immunofluorescence - Adiponectin antibody [19F1] （ab22554）Image courtesy of an anonymous Abreview.
  ab22554 staining Adiponectin in human adipose stem cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X ａnd then blocked using 4% serum for 1 hour. Samples were then incubated with primary antibody at 1/500 for 1 hour 30 minutes. The secondary antibody used was a goat IgG conjugated to Alexa Fluor® 488 （green） used at a 1/500 dilution.

  See Abreview

• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections）-Anti-Adiponectin antibody [19F1]（ab22554）
  Immunohistochemistry was performed on biopsies of deparaffinized Human skin tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate （pH6.0） buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Adiponectin ab22554 or without primary antibody （negative control） overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST ａnd endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody ａnd SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin ａnd prepped for mounting.
  
  
• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections）-Anti-Adiponectin antibody [19F1]（ab22554）
  Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate （pH6.0） buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Adiponectin ab22554 or without primary antibody （negative control） overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST ａnd endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody ａnd SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin ａnd prepped for mounting.
  
  

References for Anti-Adiponectin antibody [19F1] （ab22554）