Anti-Actin α /α-SMA，肌动蛋白α抗体

• Product nameAnti-Actin antibody [ACTN05 （C4）]See all Actin primary antibodies ...
• Description
  Mouse monoclonal [ACTN05 （C4）] to Actin
• SpecificityThis antibody reacts with all six known vertebrate isoforms of actin. It also reacts with two cytoplasmic actins （beta, lambda） which are highly homologous to one another but which differ from the muscle actins at about 25 amino acidresidues.
• Tested applicationsFlow Cyt, IP, WB, IHC-Fr, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Rabbit, Cow, Dog, Human, Pig, Dictyostelium discoideum, Physarum polycephalum
  Predicted to work with: Chicken
• Immunogen

  Chicken gizzard actin.

• Positive controlHeLa cells.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot ａnd store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
• Concentration500 µl at 0.2 mg/ml
  
• PurityProtein G purified
• Purification notesProduced from ascites then purified by protein G.
• ClonalityMonoclonal
• Clone numberACTN05 （C4）
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Tags & Cell Markers
  • Subcellular Markers
  • Cytoskeleton
  • Actin

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Troponin

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Tropomyosin

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin Crosslinking

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin Binding Proteins

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin Assembly

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Death receptors & ligands
  • RIP

Applications

Target

• FunctionActins are highly conserved proteins that are involved in various types of cell motility ａnd are ubiquitously expressed in all eukaryotic cells.
• Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 （NEM3） [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity ａnd onset, ａnd abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg （cores） within myofibers. Core lesions are unstructured ａnd poorly circumscribed.
  Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments （MPCETM） [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils ａnd mitochondria, ａnd replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, ａnd necrosis are absent.
  Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion （CFTD） [MIM:255310]; also known as congenital fiber-type disproportion myopathy （CFTDM）. CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific ａnd can be found in many different myopathic ａnd neuropathic conditions.
• Sequence similaritiesBelongs to the actin family.
• Cellular localizationCytoplasm > cytoskeleton.
• Information by UniProt
• Database links
  • Entrez Gene: 58 Human
  • Entrez Gene: 11459 Mouse
  • Entrez Gene: 29437 Rat
  • Omim: 102610 Human
  • SwissProt: P68133 Human
  • SwissProt: P68134 Mouse
  • SwissProt: P68136 Rat
  • Unigene: 1288 Human

  see all
• Alternative names
  • ACTA antibody
  • ACTA1 antibody
  • Actin alpha skeletal muscle antibody

  see all

Anti-Actin antibody [ACTN05 （C4）] images

• 

  Western blot - Actin antibody [ACTN05 （C4）] （ab3280）
  
  
  Predicted band size : 42 kDa
  
• 

  Western blot - Actin antibody [ACTN05 （C4）] （ab3280）
  All lanes : Anti-Actin antibody [ACTN05 （C4）] （ab3280） at 1/2000 dilution
  
  Lane 1 : Mouse liver whole tissue lysate （mouse 1）.
  Lane 2 : Mouse liver whole tissue lysate （mouse 2）.
  Lane 3 : Mouse liver whole tissue lysate （mouse 3）.
  
  Lysates/proteins at 50 µg per lane.
  
  Secondary
  An HRP-conjugated goat anti-mouse IgG polyclonal. at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 42 kDa
  

  This image is courtesy of an anonymous Abreview

  See Abreview

• 

  Immunohistochemistry （Frozen sections） - Actin antibody [ACTN05 （C4）] （ab3280）This image is courtesy of an anonymous Abreview
  ab3280 staining Actin in mouse heart tissue sections by IHC-Fr （formaldehyde-fixed frozen sections）. Tissue samples were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 ａnd blocked with 5% serum for 1 hour at 25°C. The sample was incubated with primary antibody （1/250 in PBS-Tween） at 4°C for 12 hours. An Alexa Fluor^®488-conjugated Goat polyclonal to mouse IgG （1/250） was used as secondary antibody. Nuclei were stained with DAPI.

  See Abreview

• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Actin antibody [ACTN05 （C4）] （ab3280）

  ab3280 （1µg/ml） staining actin in human colon using an automated system （DAKO Autostainer Plus）. Using this protocol there is strong cytoplasmic staining throughout the tissue.
  Sections were rehydrated ａnd antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes （containing casein 0.25% in PBS） then incubated with primary antibody for 20 min ａnd detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin ａnd coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration ａnd incubation time is recommended. Signal amplification may be required.

• 

  Immunocytochemistry/ Immunofluorescence - Actin antibody [ACTN05 （C4）] （ab3280）
  ICC/IF image of ab3280 stained Hepp cells. The cells were 4% formaldehyde fixed （10 min） ａnd then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells ａnd block non-specific protein-protein interactions. The cells were then incubated with the antibody （ab3280, 10µg/ml） overnight at +4°C. The secondary antibody （green） was Alexa Fluor® 488 goat anti-mouse IgG （H+L） used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes （red） at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei （blue） at a concentration of 1.43µM.
  
  
• 

  Immunocytochemistry/ Immunofluorescence - Actin antibody [ACTN05 （C4）] （ab3280）
  ICC/IF image of ab3280 stained HeLa cells. The cells were 100% methanol fixed （5 min） ａnd then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells ａnd block non-specific protein-protein interactions. The cells were then incubated with the antibody （ab3280, 5µg/ml） overnight at +4°C. The secondary antibody （green） was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed （ab96879） used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes （red） at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei （blue） at a concentration of 1.43µM.
• 

  Flow Cytometry - Anti-Actin antibody [ACTN05 （C4）] （ab3280）
  Overlay histogram showing HeLa cells stained with ab3280 （red line）. The cells were fixed with 80% methanol （5 min） ａnd then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody （ab3280, 1µg/1x10⁶cells） for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG （H+L） （ab96879） at 1/500 dilution for 30 min at 22ºC. Isotype control antibody （black line） was mouse IgG1 [ICIGG1] （ab91353, 2µg/1x10⁶ cells） used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde （10 min）/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  
  

Protocols

• Recommended IP protocol with ab3280

References for Anti-Actin antibody [ACTN05 （C4）] （ab3280）