Anti-Ack1，Ack1抗体

• Product nameAnti-ACK1 antibodySee all ACK1 primary antibodies ...
• Description
  Rabbit polyclonal to ACK1
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Rat, Human
  Predicted to work with: Mouse, Cow, Dog, Rhesus monkey
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1000 to the C-terminus of Human ACK1.

  Read Abcam＇s proprietary immunogen policy

  （Peptide available as ab74487.）

• Positive controlThis antibody gave a positive signal in the following Whole Cell Lysates: HeLa, HepG2, PC12, SHSY-5Y, T24/83, HT 1080

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term （1-2 weeks）. Aliquot ａnd store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• ClonalityPolyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Other

  • Signal Transduction
  • Signaling Pathway
  • G Protein Signaling
  • Small G Proteins
  • Other

  • Signal Transduction
  • Growth Factors/Hormones
  • EGF

  • Epigenetics ａnd Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Testosterone

  • Cancer
  • Cell cycle
  • Kinases/phosphatases
  • Other

  • Cancer
  • Growth factors
  • EGF

Applications

Target

• FunctionNon-receptor tyrosine kinase that regulates the activity of a number of proteins by tyrosine phosphorylation especially proteins critical for cell survival, cell growth, ａnd proliferation. Activates AKT1 by phosphorylating it on ＇Tyr-176＇ resulting in its activation. Phosphorylates AR on ＇Tyr-267＇ ａnd ＇Tyr-363＇ ａnd promotes its recruitment to the androgen-responsive enhancers （AREs）. Phosphorylates WWOX on ＇Tyr-287＇. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- ａnd mono-ubiquitin ａnd regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function ａnd plasticity ａnd in brain development.
• Tissue specificityThe Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia （ADH）, ductal carcinoma in situ （DCIS）, invasive ductal carcinoma （IDC） ａnd lymph node metastatic （LNMM） stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
  Contains 1 CRIB domain.
  Contains 1 protein kinase domain.
  Contains 1 SH3 domain.
  Contains 1 UBA domain.
• DomainThe EBD （EGFR-binding domain） domain is necessary for interaction with EGFR.
  The SAM-like domain is necessary for NEDD4-mediated ubiquitination. Promotes membrane localization ａnd dimerization to allow for autophosphorylation.
  The UBA domain binds both poly- ａnd mono-ubiquitin.
• Post-translational
  modificationsAutophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC.
  Ubiquitinated by NEDD4. Its EGF-induced degradation is EGFR activation-dependent ａnd is processed by lysosomes, not proteasomes.
• Cellular localizationCell membrane. Nucleus. Endosome. Cell junction > adherens junction. Cytoplasmic vesicle membrane. The Tyr-284 phosphorylated form is expressed both in the membrane ａnd nucleus. Co-localizes with EGFR on the endosomes.

• Target information above from: UniProt accession Q07912The UniProt Consortium
  The Universal Protein Resource （UniProt） in 2010
  Nucleic Acids Res. 38:D142-D148 （2010） .

  Information by UniProt
• Database links
  • Entrez Gene: 280710 Cow
  • Entrez Gene: 10188 Human
  • Entrez Gene: 51789 Mouse
  • Entrez Gene: 303882 Rat
  • Omim: 606994 Human
  • SwissProt: Q17R13 Cow
  • SwissProt: Q07912 Human
  • SwissProt: O54967 Mouse

  • SwissProt: Q5U2X5 Rat
  • Unigene: 518513 Human
  • Unigene: 251115 Mouse
  • Unigene: 98335 Rat

  see all
• Alternative names
  • Acetate kinase 1 antibody
  • Acetokinase 1 antibody
  • ACK 1 antibody

  • ACK antibody
  • ACK-1 antibody
  • ACK1 antibody
  • ACK1_HUMAN antibody
  • Activated Cdc42 associated kinase 1 antibody
  • Activated CDC42 kinase 1 antibody
  • Activated p21cdc42Hs kinase antibody
  • FLJ44758 antibody
  • FLJ45547 antibody
  • p21cdc42Hs antibody
  • TNK 2 antibody
  • TNK2 antibody
  • Tyrosine kinase non receptor 2 antibody
  • Tyrosine kinase non receptor protein 2 antibody
  • Tyrosine kinase non-receptor protein 2 antibody

  see all

Anti-ACK1 antibody images