Anti-5-HT，5-羟色胺抗体

• Product nameAnti-5HT2A Receptor antibodySee all 5HT2A Receptor primary antibodies ...
• Description
  Rabbit polyclonal to 5HT2A Receptor
• Specificityab16028 detects rat 5HT2A receptor protein at the expected 53kDa. Three further bands are detected in WB on rat brain lysate at ~60, 75 ａnd 110kDa. These extra larger bands might result following glycosylation of the 5HT2A receptor protein. We are unsure of the identity of these additional bands. ab16028 works well in IHC where specific immunoreactivity is seen in the expected brain areas.
• Tested applicationsIHC （PFA fixed）, WB, IHC-FoFr, IHC-Fr, IHC-FrFl more details
• Species reactivity
  Reacts with: Mouse, Rat, Cow
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat 5HT2A Receptor.

  Read Abcam＇s proprietary immunogen policy

  .

• Positive controlThis antibody gave a positive signal on the following tissue lysates: Rat Hippocampus Rat Brain Mouse Brain

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term （1-2 weeks）. Aliquot ａnd store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration100 µg at 1 mg/ml
  
• PurityImmunogen affinity purified
• ClonalityPolyclonal
• IsotypeIgG
• Research Areas
  • Neuroscience
  • Neurotransmission
  • Receptors / Channels
  • GPCR
  • Serotonin Receptors

  • Metabolism
  • Types of disease
  • Obesity

Applications

• Application notesIHC-Fr: 1/200 （PMID 19295659）.
  IHC-FoFr: Use at 1:100 dilution with TSA amplification. Perfusion fix tissue with 4% paraformaldehyde + 15% saturated picric acid, post-fix in the same fixative overnight, then cryoprotect in 20% sucrose ａnd freeze in OCT.
  
  WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa （predicted molecular weight: 53 kDa）.
  Immunohistochemistry （PFA fixed）: 1/500 （from PubMed:17442243）.
  
  A customer has tested this product in IHC-P ａnd did not find it suitable for this application （see enquiries section）.
  
  Not tested in other applications.
  Optimal dilutions/concentrations should be determined by the end user.

Target

• FunctionThis is one of the several different receptors for 5-hydroxytryptamine （serotonin）, a biogenic hormone that functions as a neurotransmitter, a hormone, ａnd a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, ａnd control of aldosterone production.
• Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
• DomainThe PDZ domain-binding motif is involved in the interaction with INADL, CASK, APBA1, DLG1 ａnd DLG4.
• Cellular localizationCell membrane. Localizes to the post-synaptic thickening of axo-dendritic synapses.
• Information by UniProt
• Database links
  • Entrez Gene: 407230 Cow
  • Entrez Gene: 15558 Mouse
  • Entrez Gene: 29595 Rat
  • SwissProt: Q75Z89 Cow
  • SwissProt: P35363 Mouse
  • SwissProt: P14842 Rat
  • Unigene: 214351 Mouse
  • Unigene: 10294 Rat

  see all
• Alternative names
  • 5 HT 2 antibody
  • 5 HT 2A antibody
  • 5 HT2 receptor antibody

  see all

Anti-5HT2A Receptor antibody images

• 

  Immunohistochemistry - Free Floating - 5HT2A Receptor antibody （ab16028）Lora Heisler, Dept of Clinical Biochemistry, United Kingdom
  ab16028 5HT2AR staining in IHC free-floating protocol on rat brain tissue. [a] coronal section through hypothalamus. [b] High-power magnification of the small box in panel [a]. Nuclear staining was observed in the Arcuate nucleus （Arc）, but not in the Ventromedial nucleus （VMH） of the hypothalamus. [c] Pyramidal like neurons were stained by ab16028. [d] Nuclear-like staining in the nucleus of the solitary tract （sol）. Scale bars in [b] ａnd [c] =10um ａnd in [d]=40um
• 

  Immunohistochemistry （PFA perfusion fixed frozen sections） - 5HT2A Receptor antibody （ab16028）Sophie Pezet, CNRS, France
  ab16028 staining of 5HT2A receptor in free-floating rat brain cortex sections. Similar punctate staining was observed in rat hypothalamus （data not shown）. Rats were intracardially perfused with 4% paraformaldehyde. Tissues were post-fixed overnight in the same fixative prior to cryoprotection in 20% sucrose ａnd freezing in OCT. 30 µm sections were cut for use in free-floating IHC ａnd incubated with ab16028 （1:300） overnight at room temperature. Secondary antibody （anti-rabbit biotin, 1:300） was incubated for 1 hour at room temperature.
• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - 5HT2A Receptor antibody （ab16028）
  IHC image of 5HT2A staining in rat brain FFPE section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer （pH6, epitope retrieval solution 1） for 20 mins. The section was then incubated with ab16028, 5µg/ml, for 15 mins at room temperature ａnd detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin ａnd mounted with DPX.
• 

  Western blot - 5HT2A Receptor antibody （ab16028）
  All lanes : Anti-5HT2A Receptor antibody （ab16028） at 1 µg/ml
  
  Lane 1 : Rat Hippocampus Tissue Lysate
  Lane 2 : Brain （Rat） Tissue Lysate
  Lane 3 : Brain （Mouse） Tissue Lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed （HRP） at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 53 kDa
  Observed band size : 55 kDa （why is the actual band size different from the predicted?）
  Additional bands at : 100 kDa,34 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 5 minutes
• 

  Immunohistochemistry （Frozen sections） - Anti-5HT2A Receptor antibody （ab16028）Image from Johansson S et al., PLoS One. 2012;7（7）:e41852. Epub 2012 Jul 27. Fig 6.; doi:10.1371/journal.pone.0041852; July 27, 2012, PLoS ONE 7（7）: e41852.
  Immunohistochemical analysis of ischaemic rat anterior cerebral artery, staining 5HT2A Receptor with ab16028.
  
  Sections were blocked with 5% donkey serum ａnd 1% BSA. Samples were incubated with primary antibody （1/200） ａnd a DyLight®488-conjugated donkey anti-rabbit IgG （1/200） was used as a secondary antibody.

References for Anti-5HT2A Receptor antibody （ab16028）