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磷酸化胰岛素受体底物-1抗体3203
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品牌名称：$brandModel.Title型号：3203 原产地：中国大陆 更新时间：2026/11/10 8:31:36
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详细内容
英文名称 TRA1 variant
中文名称 肿瘤排斥抗原gp96 1 变异体抗体
别 名 tumor rejection antigen (gp96) 1 variant; TRA1; HSP gp96; heat shock protein gp96 precursor; 94 kDa glucose regulated protein; ECGP; Endoplasmin; Endothelial cell (HBMEC) glycoprotein; Glucose regulated protein 94; Glucose regulated protein 94kDa; gp96; gp96 homolog; GRP 94; Heat shock protein 90 kDa beta member 1; HSP90B1; Stress inducible tumor rejection antigen GP96; TRA1; Tumor rejection antigen 1; Tumor rejection antigen gp96; ENPL_HUMAN; Endoplasmin.
研究领域 肿瘤 免疫学 神经生物学 信号转导 细胞凋亡 新陈代谢
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Mouse, Rat, (predicted: Human, Chicken, Pig, Horse, Rabbit, Guinea Pig, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 63kDa
细胞定位 细胞浆
性 状 Liquid
浓 度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human TRA1 variant:
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍
产品图片 Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Pancreas (Mouse) Lysate at 40 ug
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Lane 5: Testis (Rat) Lysate at 40 ug
Primary: Anti-TRA1 variant (bs-0149R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100 kD
Observed band size: 100 kD
Paraｆormaldehyde-fixed, paraffin ｅmbedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRA1 variant) Polyclonal Antibody, Unconjugated (bs-0149R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraｆormaldehyde-fixed, paraffin ｅmbedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRA1 variant) Polyclonal Antibody, Unconjugated (bs-0149R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraｆormaldehyde-fixed, paraffin ｅmbedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRA1 variant) Polyclonal Antibody, Unconjugated (bs-0149R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
热门标签：磷酸化胰岛素受体底物-1抗体,雅吉抗体,一抗
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