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RNA聚合酶II抗体Ys-6972R
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品牌名称：$brandModel.Title型号：Ys-6972R 原产地：中国大陆 更新时间：2026/11/10 8:31:36
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产品编号Ys-6972R
英文名称RNA polymerase II
中文名称RNA聚合酶II抗体
别 名POLR2A; POLR2; DNA directed RNA polymerase II A; DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II subunit A; RPB1_HUMAN.
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, )
产品应用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量217kDa
细胞定位细胞核
性 状Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human Pol II/RNA polymerase II: 101-200/1970
亚 型IgG
纯化方法affinity purified by Protein A
缓 冲 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMedPubMed
产品介绍RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the tranｓcription of protein-coding genes. Tranｓcription initiation requires Pol II-mediated recruitment of tranｓcription machinery to a target promoter, thereby allowing tranｓcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, ｆorms the active center of the Pol II enzyme. Additionally, the large subunit participates in ｆorming the DNA-binding domain of Pol II, a groove that is necessary for tranｓcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent tranｓcription elongation cannot occur.
Function:
DNA-dependent RNA polymerase catalyzes the tranｓcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II tranｓcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of tranｓcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conｆormations at each step of nucleotide addition. During tranｓcription elongation, Pol II moves on the template as the tranｓcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platｆorm for assembly of factors that regulate tranｓcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and tranｓcriptase for the viral RNA circular genome.
Subunit:
Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. The phosphorylated C-terminal domain interacts with FNBP3 and SYNCRIP. Interacts with SAFB/SAFB1. Interacts with CCNL1 and MYO1C (By similarity). Interacts with CCNL2 and SFRS19. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with PAF1. Interacts (via C-terminus) with FTSJD2, CTDSP1 and SCAF8. Interacts via the phosphorylated C-terminal domain with WDR82 and with SETD1A and SETD1B only in the presence of WDR82. Interacts with ATF7IP. When phosphorylated at 'Ser-5', interacts with MEN1; the unphosphorylated ｆorm, or phosphorylated at 'Ser-2' does not interact. Interacts with DDX5.
Subcellular Location:
Nucleus.
Post-translational modifications:
Dephosphorylated by the protein phosphatase CTDSP1. [PTM] Ubiquitinated by WWP2 leading to proteasomal degradation (By similarity). [PTM] Methylated at Arg-1810 by CARM1. Methylation occurs only when the CTD is hypophosphorylated, and phosphorylation at Ser-1805 and Ser-1808 prevent methylation (in vitro). It is assumed that methylation occurs prior to phosphorylation and tranｓcription initiation. CTD methylation may facilitate the expression of select RNAs.
热门标签：RNA聚合酶II抗体,抗体
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