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pickering 柱后衍生试剂
点击次数:9发布时间:2023/11/27
更新日期:2024/4/25 15:20:47
生 产 地:中国大陆
产品型号:pickering
详细内容
Pickering/皮克林钠离子交换柱法测氨基酸Pickering柱后衍生试剂
OPA 用于衍生分析氨基酸是1971年提出。图 1 为 OPA 与一氨基酸的反应方程式。
室温下, OPA (Pickering O120) 在(Pickering 3710-2000)环境中与一氨基酸于1 min 内即可生成具有强荧光的衍生产物。OPA 本身不发荧光的特性使其在色谱分离时不形成干扰峰。OPA 只能与经次氯酸钠等氧化剂氧化开环后的二氨基酸反应,也可与 FMOC - Cl 联用衍生二氨基酸。OPA 衍生法因样品制备简单、衍生反应迅速、灵敏度高及易于实现自动化操作等优点而成为柱衍生反相高效液相色谱法中使用广泛的方法。
OPA 衍生法(用于检测一氨基酸)
试剂: 300 mgOPA (Pickering O120), 2 g Thiofluor?(Pickering 3710-2000), 3 mL 30 % Brij® 35 溶于 950 mLOPA稀释液(Pickering OD104)中
反应器: 45 °C, 0.15 mL
衍生试剂流速: 0.3mL/min
检测器: 荧光检测器
λex= 330nm, λem=465 nm
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