产 品 说 明 | |||
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类型:Primary antibodies
产品名称:Collagen II antibody 名称:Collagen II antibody 兔多克隆抗体
货号:orb10436
受体:Rabbit
克隆能力:Polyclonal
测试应用:WB,IHC-P,IF/ICC
规格:100ug、200ug
适用种属:Human、Mouse、Rat、Pig
一、功能描述
(1)工作原理:DEK-1001型COD在线水质分析仪测量方法采用国家标准GB11914-89水质-化学需氧量测定(重铬酸钾法)。水样、重铬酸钾、硫酸银(催化剂使直链脂肪族化合物氧化更充分)和浓硫酸的混合液在消解池中被加热到175℃,在此期间铬离子作为氧化剂从VI价被还原成III价而改变了颜色,颜色的改变度与样品中有机化合物的含量成对应关系,仪器通过比色换算直接将样品的COD显示出来。
(2)DEK-1001型COD在线水质分析仪具有以下基本功能:
- 可设定、校对和显示时间,包括年、月、日和时、分;
- 具有设备断电、仪器漏液、试样无法导入反应器等系统异常情况的报警功能并显示故障内容。同时,停止运行直至系统被重新启动;
- 每次测量结束后,自动清洗前处理装置、仪器管路、阀门等部件;
- 断电、断水的自动保护和来电、来水自动恢复的功能;
- 定时清洗、定时做样功能;
- 触摸屏查看更直观,操作更方便;
- (0 -200)mg/L,(0 -500)mg/L,(0-1000)mg/L三档量程自动切换功能。
二、性能描述
独特的设计,使本产品较之同类产品具有更低故障率、更低维护量、更低试剂消耗量以及更高的性价比。
1—选择阀组件:选择试剂采样时序;
2—计量组件:通过可视光电系统实现试剂精确计量,克服蠕动泵泵管由于磨损引起的定量误差;同时实现微量试剂的精确定量,大大减少试剂用量;
3—进样组件:蠕动泵负压吸入,在试剂与泵管之间总是存在一个空气缓冲区,避免泵管腐蚀;
4—密封消解组件:高温高压消解体系,加快反应进程,克服敞口系统腐蚀性气体挥发对设备的腐蚀;
5—试剂管:采用进口改型聚四氟乙烯透明软管,管径大于1.5mm,减少水样颗粒堵塞几率。
三、技术特点
(1)采用国际领先光电定量系统,用样更精更准;
(2)采用国际领先切阀采样系统,摒弃原始的电磁阀方式。切阀采样系统采用转动取样方法,管路无电磁阀压力的老化,故障率极低,维护费用少;
(3)采用蠕动泵最新技术,故障率极低,蠕动泵管每分钟连续转动50次,寿命达1万小时;
(4)采用国际领先电源保护技术,能够适应电网不稳定的环境;
(5)具有掉电保护功能,掉电时仪器能停止一切工作,上电可自动复位;
(6)支持掉电存储功能,掉电数据不丢失;
(7)进样管路完全采用3氟、4氟材料。耐酸、耐高温、耐腐蚀;
(8)自动漏液报警功能、超出测量范围报警功能、智能故障报警功能,提示用户管理和维护。
四、技术参数
测量方法: 重铬酸钾高温消解,比色测定 (国家标准GB11914-89) | |||
测试量程: 0-1000mg/L( 可根据要求扩展) | |||
检测下线: 15 mg/L | |||
分辨率: <1mg/L | |||
准确度: 示值误差不超过10% | |||
重复性: 相对标准偏差不超过5% | |||
零点漂移: ≤±5 %/24h | |||
量程漂移: ≤±10 %/24h | |||
消解温度: 165摄氏度,可设定 | |||
样品流速: 最小为0.1L/h | |||
用户保养: 保养间隔>1个月,每月约1小时;维护简单方便 | |||
环境影响: 水质极端情况(如高悬浮物、杂质、漂浮物)下不会损坏 | |||
通信接口: RS232数字接口,4~20mA模拟信号输出 | |||
外型尺寸 | 高1400×宽560×深450mm | 重量 | 40千克 |
电源 | AC(220±10%)V,(50±10%)Hz,5A | 功率 | 80瓦 |
环境温度 | 5~40摄氏度 | 环境湿度 | ≤90%(不结露) |
大气压力 | 86千帕~106千帕 | 采样排口 | 距离L≤15m,落差H≤6m |
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MTT一般现用现配,过滤后4℃,避光保存2周有效,或保存在-20℃长期保存;避免反复冻融,最好小剂量分装,用避光袋、锡箔纸包裹;一般把MTT粉分装在EP管,再现用现配,当MTT变为灰绿色时绝不能再用; MTT致癌,用时最好带透明薄膜手套。
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培养基保存于4℃冰箱中,培养基内CO2 会逐渐溢出,造成培养基越来越偏碱性。而培养基中酸碱指示剂(通常为phenol red)的颜色也会随碱性增加而更偏暗红。
人脑瘤细胞 SF17细胞 SF17细胞状态 http://www.afzhan.com/st133337/Product_4631261.html
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培养基偏碱后再用于细胞培养将造成细胞生长停滞或死亡。培养基偏碱时,可以通入无菌过滤的CO2,以调整pH值。
小鼠胚胎成纤维细胞 MEF细胞价格 http://www.afzhan.com/st133337/Product_3324614.html
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人多发性骨髓瘤细胞 RPMI 8226细胞 http://www.afzhan.com/st133337/Product_3324637.html
根据细胞类型、培养方式和生产工艺等特点所定制的培养基,即个性化培养基。个性化培养基在国外生物制药企业被普遍采用,个性化培养基可以为细胞生长提供充足的营养物质,能提高细胞的生长速率、培养密度、以及延长细胞维持时间;
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也可以为细胞生长提供均衡的营养供给,减少细胞有害代谢物质的积累,降低对细胞生长的危害;
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同时对贴壁细胞而言,能增加细胞的贴壁性,并降低培养过程中剪切力对细胞的损伤;个性化培养基可以根据各户的特殊需求减少或不使用动物源成分,从而使生物制品安全性更有保障。
全氟消解罐又叫COD样品消解仪、消解器、高压消解罐、在海洋研究所用于海洋样品检测尤为广泛。应用于气象,液相,等离子光质谱仪,原子吸收和原子荧光等化学分析方法的样品前处理,用于消解农残,食品,稀土,水产品,有机物种的Pb,Cu,Zn,Ga,Rb,Hg等重金属。案例:清华大学:COD检测。
1.耐高低温性:可使用温度-200℃~+250℃。建议在烘箱中180度内使用(全氟消解罐)
2.使用方便精密设备加工内壁光滑,不挂水,不混配,密封性能好
3.消解效率高,能力强,可消解许多传统方法难以消解的样品
4.消耗酸溶剂少,空白值低,提高分析的准确度和精密度
5.采用全聚四氟乙烯材质加工,避免了在实验中,混入重金属,从而污染样品,防污染:金属元素空白值低。
6.外观纯白色。
7.耐腐蚀:耐强酸、强碱、王水和各种有机溶剂,且无溶出、吸附和析出现象。
8.绝缘性:不受环境及频率的影响,介质损耗小,击穿电压高。
9.耐大气老化,耐辐照和较低的渗透性。
10.自润滑性:具有塑料中最小的摩擦系数。
11.表面不粘性:是一种表面能最小的固体材料。
12.机械性质较软,具有非常低的表面能。
13.无毒害:具有生理惰性
销售部:
联系人: 季小姐
TEL:138 138 88374 025-8559 7772
FAX:025-5899 4772 E-mail:1826176783@qq.com
【Discovery 专业型分析天平 DV 分析天平(内校)0.00001/0.0001g的参数说明】 | |||||||||||||||||||||||
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人棘球蚴IgM抗体酶联免疫分析(ELISA)
试剂盒使用说明书
本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中棘球蚴IgM抗体的含量。
实验原理:
本试剂盒应用双抗原夹心法测定标本中人棘球蚴IgM抗体水平。用纯化的人棘球蚴IgM抗原包被微孔板,制成固相抗原,往包被抗原的微孔中依次加入棘球蚴IgM抗体,再与HRP标记的棘球蚴IgM抗原结合,形成抗原-抗体-酶标抗原复合物,经过洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的棘球蚴IgM抗体呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中人棘球蚴IgM抗体浓度。
试剂盒组成:
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样本处理及要求:
1. 血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。
2. 血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。
3. 尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。
4. 细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5. 组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
6. 标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.
7. 不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
操作步骤
1. 标准品的稀释与加样:在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀;然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀;然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀;混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉。(稀释后各孔加样量都为50μl,浓度分别为90pg/ml,60pg/ml ,30 pg/ml,15pg/ml , 7.5pg/ml。
2. 加样:分别设空白孔(空白对照孔不加样品及酶标试剂,其余各步操作相同)、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl(样品最终稀释度为5倍)。加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀。
3. 温育:用封板膜封板后置37℃温育30分钟。
4. 配液:将30(48T的20倍)倍浓缩洗涤液用蒸馏水30(48T的20倍)倍稀释后备用。
5. 洗涤:小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干。
6. 加酶:每孔加入酶标试剂50μl,空白孔除外。
7. 温育:操作同3。
8. 洗涤:操作同5。
9. 显色:每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.
10. 终止:每孔加终止液50μl,终止反应(此时蓝色立转黄色)。
11. 测定:以空白空调零,450nm波长依序测量各孔的吸光度(OD值)。 测定应在加终止液后15分钟以内进行。
注意事项:
1. 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存。
2. 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果。
3. 各步加样均应使用加样器,并经常校对其性,以避免试验误差。一次加样时间控制在5分钟内,如标本数量多,推荐使用排枪加样。
4. 请每次测定的同时做标准曲线,做复孔。如标本中待测物质含量过高(样本OD值大于标准品孔孔的OD值),请先用样品稀释液稀释一定倍数(n倍)后再测定,计算时请乘以总稀释倍数(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物请避光保存。
7. 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.
8. 所有样品,洗涤液和各种废弃物都应按传染物处理。
9. 本试剂不同批号组分不得混用。
10. 如与英文说明书有异,以英文说明书为准。
计算:
以标准物的浓度为横坐标,OD值为纵坐标,
在坐标纸上绘出标准曲线,根据样品的OD
值由标准曲线查出相应的浓度;再乘以稀释
倍数;或用标准物的浓度与OD值计算出标
准曲线的直线回归方程式,将样品的OD值
代入方程式,计算出样品浓度,再乘以稀释
倍数,即为样品的实际浓度。
(此图仅供参考)
试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.92以上。
2.批内与批见应分别小于9%和15%
检测范围:
3pg/ml -100 pg/ml
保存条件及期:
1.试剂盒保存:;2-8℃。
2.期:6个月
FOR RESEARCH USE ONLY
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Drug Names
Generic Name:Human echinococcosis IgM antibody (ECH-IgM) ELISA Kit.
Purpose
This kit allows for the determination of ECH-IgM concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human ECH-IgM level in the sample,use Purified Human ECH-IgM antigen to coat microtiter plate wells, make solid-phase antigen, then add ECH-IgM to wells, Combined ECH-IgM antigen which With HRP labeled , become antigen - antibody - enzyme- antigen complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ECH-IgM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
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| (20ml×20 fold) ×1bottle | (20ml×30 fold) | |
Specimen requirements
1. serum- coagulation at room temperature 10-20 mins,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2. plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3. Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4. cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS(PH7.2-7.4), Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5. Tissue samples- After cutting samples, check the weight,add PBS(PH7.2-7.4), Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS(PH7.4), Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separately. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 90pg/ml,60pg/ml ,30 pg/ml,15pg/ml , 7.5pg/ml)
2.add sample:Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1. The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2. washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3. add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4. if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).
5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6. The substrate evade the light preservation.
7. Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8. All samples, washing buffer and each kind of reject should according to infective material process.
9. Do not mix reagents with those from other lots.
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Calculate
This chart for reference only
Assay range
3pg/ml -100 pg/ml
Storage and validity
1.Storage: 2-8℃.
2.validity: six months.