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测量范围: 球类:16-177公里/小时 10-110英里/小时 车类:16-320公里/小时 10-200英里/小时 作用距离: 针对球类:0-22.5米 针对车: 0-390米 测量精度:+/-1.0MPH +/-2.0KPH 单位显示:公里/小时(KPH)或 英里/小时(MPH) 重量:539克 市价:¥5400.00 优惠价:来电洽询 供货情况:现货 操作方法: 正确安装电池后,合上电池后盖, 轻按显示屏下方电源开关,沿物体运动方向瞄准物体并按下操作键,即时,运动物体的速度便会实时显示在显示屏上面! |
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介绍:贺利氏heraeus氘灯SD3651/04J替代Hitachi 日立LC-DAD氘灯890-2430
德国贺利氏特种光源公司生产的氘灯具有输出稳定,低噪声和低漂移的特性。作为众多国际知名仪器厂商的OEM氘灯制造商,贺利氏特种光源公司的氘灯能够与市售的大部分仪器品牌相匹配,适用于各种型号的进口和国产液相色谱仪、紫外分光光度计以及毛细管电泳仪欢迎来到沈阳镁汇科技有限公司,进口氘灯全国总经销:waters沃特斯氘灯、shimadzu岛津氘灯、agilent安捷伦氘灯、pe氘灯、Hitachi日立氘灯、贺利氏氘灯Heraes氘灯、varian瓦里安氘灯、dionex戴安氘灯、beckman贝克曼氘灯、knauer诺尔氘灯、gilson氘灯、SSIAltex?氘灯、Thermo氘灯、英麟氘灯、Biotage氘灯、Isco氘灯、普析通用、福立氘灯、伍丰氘灯、创新通恒/北分瑞利氘灯等。购买前请确认:仪器厂商、仪器/检测器型号、原装灯货号,原装灯、置换灯的具体价格请致电本公司aaa我公司承诺,自氘灯到货之日起,若安装到设备提示能量值低或无法点亮,我公司负责更换新灯并承担往返运费。到货6个月内为质保期,若我公司出售的产品在质保期内出现能量值降低等状况,我公司检测,若为氘灯质量问题,我公司负责更换新灯并承担往返运费。沈阳镁汇科技有限公司地址:沈阳市铁西区北滑翔路21号电话: 传真:手机:aaa 网址:aaa购买链接:https://item.taobao.com/item.htm?spm=a1z10.1-c-s.w4004-12942303260.6.309a4facISs8mp&id=575480673011
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电气连接: | 电缆、Bendix、特殊接口 |
类型: | 表压,绝压 |
特点: | 数字补偿,ICSensors超稳技术,高精度,CE认证 |
供电电源: | 5~30V(视输出而定) |
输出: | 4~20mA 或 电压输出 |
精确度: | ± 0.05% |
工作温度范围: | 补偿:-20℃~85℃ |
量程: | 0-5,15,30,50,100,300,500,1000,3000,5000,10,000(psi) |
典型应用: | 军事,航天,航空测试台,校验装置,工业设备 |
温州瑞昕仪器有限公司
温州瑞昕仪器有限公司是从事工业用检测仪器、理化分析仪器、计量仪器产品的销售、服务、维修一体的公司。欢迎您的咨询订购,联系方式:
公司电话:0 5 7 7-6 6 8 8 6 0 1 7
传真:0 5 7 7-6 6 8 8 6 0 1 9
邮箱:rxin17@163.com
网址:http://www.rxin17.com/
联系人:
陈进碗 手机:1 3 7 5 8 7 6 8 8 1 3 QQ:124677072
【详细说明】
美国Agilent(安捷伦) 液相柱 PH值2-9 产品描述:
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人广州管圆线虫抗体IgM酶联免疫分析(ELISA)
试剂盒使用说明书
本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中广州管圆线虫抗体IgM的含量。
实验原理:
本试剂盒应用双抗原夹心法测定标本中人广州管圆线虫抗体IgM水平。用纯化的人广州管圆线虫抗体IgM抗原包被微孔板,制成固相抗原,往包被抗原的微孔中依次加入广州管圆线虫抗体IgM,再与HRP标记的广州管圆线虫抗体IgM抗原结合,形成抗原-抗体-酶标抗原复合物,经过洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的广州管圆线虫抗体IgM呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中人广州管圆线虫抗体IgM浓度。
试剂盒组成:
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样本处理及要求:
1. 血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。
2. 血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。
3. 尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。
4. 细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5. 组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
6. 标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.
7. 不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
操作步骤
1. 标准品的稀释与加样:在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀;然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀;然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀;混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉。(稀释后各孔加样量都为50μl,浓度分别为72pg/ml,48 pg/ml ,24 pg/ml,12 pg/ml , 6pg/ml。
2. 加样:分别设空白孔(空白对照孔不加样品及酶标试剂,其余各步操作相同)、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl(样品最终稀释度为5倍)。加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀。
3. 温育:用封板膜封板后置37℃温育30分钟。
4. 配液:将30(48T的20倍)倍浓缩洗涤液用蒸馏水30(48T的20倍)倍稀释后备用。
5. 洗涤:小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干。
6. 加酶:每孔加入酶标试剂50μl,空白孔除外。
7. 温育:操作同3。
8. 洗涤:操作同5。
9. 显色:每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.
10. 终止:每孔加终止液50μl,终止反应(此时蓝色立转黄色)。
11. 测定:以空白空调零,450nm波长依序测量各孔的吸光度(OD值)。 测定应在加终止液后15分钟以内进行。
注意事项:
1. 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存。
2. 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果。
3. 各步加样均应使用加样器,并经常校对其性,以避免试验误差。一次加样时间控制在5分钟内,如标本数量多,推荐使用排枪加样。
4. 请每次测定的同时做标准曲线,做复孔。如标本中待测物质含量过高(样本OD值大于标准品孔孔的OD值),请先用样品稀释液稀释一定倍数(n倍)后再测定,计算时请乘以总稀释倍数(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物请避光保存。
7. 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.
8. 所有样品,洗涤液和各种废弃物都应按传染物处理。
9. 本试剂不同批号组分不得混用。
10. 如与英文说明书有异,以英文说明书为准。
计算:
以标准物的浓度为横坐标,OD值为纵坐标,
在坐标纸上绘出标准曲线,根据样品的OD
值由标准曲线查出相应的浓度;再乘以稀释
倍数;或用标准物的浓度与OD值计算出标
准曲线的直线回归方程式,将样品的OD值
代入方程式,计算出样品浓度,再乘以稀释
倍数,即为样品的实际浓度。
(此图仅供参考)
试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.92以上。
2.批内与批见应分别小于9%和15%
检测范围:
2 pg/ml -90 pg/ml
保存条件及期:
1.试剂盒保存:;2-8℃。
2.期:6个月
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Drug Names
Generic Name:Human Angiostrongylus cantonensis antibody IgM (AC-IgM) ELISA Kit.
Purpose
This kit allows for the determination of AC-IgG concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human AC-IgM level in the sample,use Purified Human AC-IgM antigen to coat microtiter plate wells, make solid-phase antigen, then add AC-IgM to wells, Combined AC-IgM antigen which With HRP labeled , become antigen - antibody - enzyme- antigen complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AC-IgM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
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| (20ml×20 fold) ×1bottle | (20ml×30 fold) | |
Specimen requirements
1. serum- coagulation at room temperature 10-20 mins,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2. plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3. Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4. cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS(PH7.2-7.4), Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5. Tissue samples- After cutting samples, check the weight,add PBS(PH7.2-7.4), Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS(PH7.4), Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separately. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 72pg/ml,48 pg/ml ,24 pg/ml,12 pg/ml , 6pg/ml)
2.add sample:Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1. The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2. washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3. add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4. if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).
5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6. The substrate evade the light preservation.
7. Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8. All samples, washing buffer and each kind of reject should according to infective material process.
9. Do not mix reagents with those from other lots.
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Calculate
This chart for reference only
Assay range
2pg/ml -90 pg/ml
Storage and validity
1.Storage: 2-8℃.
2.validity: six months.