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网址：www.asaie.cn寿力SULLUBE32空压机油 250022-669寿力空压机油87250022-669寿力空压机油87250022-670寿力空压机油250022-670寿力压缩机油RF/4000寿力空压机油 250019-662寿力空压机油02250051-153寿力空压机油寿力24KT空压机油 02250051-150寿力空压机油寿力移动空压机油 250030-757寿力空压机油SULLUBE32空压机油02250137-895油气分离器250034-112油气分离器250034-114油气分离器02250121-500油气分离器02250100-753油气分离器02250100-754油气分离器02250100-755油气分离器02250100-756油气分离器250034-116油气分离器250042-862油气分离器02250060-462油气分离器02250060-463油气分离器02250061-137油气分离器02250061-138油气分离器02250061-753油气分离器02250061-754油气分离器250034-085油气分离器02250048-734油气分离器250034-087油气分离器250034-086油气分离器250034-122油气分离器250034-134油气分离器250034-120油气分离器250034-128油气分离器250034-124油气分离器250034-123油气分离器250034-130油气分离器250034-129油气分离器02250109-319油气分离器02250109-321油气分离器250028-244油气分离器02250131-498空气滤芯02250131-499空气滤芯250018-652空气滤芯043334空气滤芯02250131-496空气滤芯040596空气滤芯02250044-537空气滤芯049301空气滤芯88290001-469空气滤芯02250125-371空气滤芯047542空气滤芯047543空气滤芯02250046-012空气滤芯02250046-013空气滤芯88290006-013空气滤芯88290006-012空气滤芯P181043空气滤芯P124860空气滤芯0409853空气滤芯0409854空气滤芯88290007-018空气滤芯02250135-149空气滤芯250007-838空气滤芯250007-839空气滤芯88290005-014空气滤芯88290001-466空气滤芯88290001-467空气滤芯88290003-111空气滤芯88290004-372空气滤芯8829001-251空气滤芯88290002-337空气滤芯88290002-338空气滤芯02250051-238空气滤芯02250051-239空气滤芯02250125-372空气滤芯882900012-371空气滤芯88290005-590空气滤芯02250168-053空气滤芯88290005-591空气滤芯02250127-684空气滤芯88290003-034空气滤芯88290003-467空气滤芯88290005-013空气滤芯88290005-014空气滤芯88290012-371空气滤芯88290012-372空气滤芯88290007-O18空气滤芯88290013-079空气滤芯250025-525油过滤器250025-526油过滤器250008-956油过滤器02250121-219油过滤器250007-842油过滤器250031-850油过滤器02250139-996油过滤器250007-665油过滤器02250155-709油过滤器02250125-367滤芯02250096-783油过滤器016742断油阀总成014098断油阀总成001684断油阀维修包88290012-847止逆阀02250078-204温控阀芯045764温控阀芯049542温控阀芯  001168温控阀芯02250092-081温控阀芯250028-762温控阀芯88290002-832温控阀芯013697温控阀芯88290022-023温控阀芯02250096-738温控阀芯88290001-006温控阀总成250025-621温控阀维修包02250105-553温控阀维修包001084温控阀维修包001076温控阀维修包826502-144温控阀O型圈02250101-372温控阀V密封圈250017-280背压调节开关02250127-402减压阀048354减压阀406929压力调节器408275压力调节器02250084-027压力调节器02250046-568压力调节器048059压力调节器045099压力调节器02250052-358压力调节器88291002-741压力调节器250023-600真空调节器02250190-454溢流阀048409压力调节器维修包250019-453压力调节器维修包041742压力调节器维修包02250049-634放空阀02250100-042放空阀250028-693放气阀250025-655放气阀250030-276放气阀041111放气阀409783放空阀250031-771放空阀维修包250031-772放空阀维修包02250045-132放空阀维修包001667放空阀维修包250020-353寿力控维修包608311-001螺旋阀维修包250006-526进气阀弹簧02250112-032控制管路过滤器408389控制管路过滤器02250112-031控制管路过滤器维修包001692控制管路过滤器维修包250031-245控制管路过滤器维修包02250115-960控制管路过滤器维修包88290018-880控制管路过滤器维修包02250117-782回油管过滤器维修包02250125-684电磁阀250038-666电磁阀250038-755电磁阀88290015-219电磁阀250038-672电磁阀02250125-657电磁阀250038-676电磁阀02250125-665电磁阀250038-673电磁阀维修包250038-730电磁阀线圈250031-738电磁阀线圈02250055-940电磁阀维修包250033-821最小压力阀02250097-598最小压力阀02250097-609最小压力阀242405最小压力阀88290016-467最小压力阀88290017-107最小压力阀88290017-287最小压力阀88290017-432最小压力阀02250046-338最小压力阀组件88290017-286止回阀88290018-146最小压力阀维修包250026-758最小压力阀维修包02250177-150最小压力阀维修包88290009-185进气阀02250110-998最小压力阀维修包250018-262最小压力阀维修包250019-444最小压力阀维修包001176最小压力阀维修包001177最小压力阀维修包250018-456最小压力阀维修包250018-459最小压力阀维修包88290018-146最小压力阀维修包88290019-707最小压力阀维修包88290012-468进气阀维修包250042-656弹簧02250056-278进气阀维修包02250046-396压力阀盖维修包02250048-363压力阀O型圈包02250051-337压力阀活塞维修包250014-656真空开关250017-991水压开关250017-992水压开关02250084-534水温开关02250050-154压差开关02250100-095高温保护开关250039-909温度传感器88290001-535急停按扭88290003-806压力传感器88290014-514压力传感器241771V型过滤器241772V型过滤器维修包040694压力调节开关407778压力调节开关250029-249进气阀维修包250017-451进气阀维修包250019-451进气阀维修包02250053-273进气阀维修包250031-438进气阀维修包02250073-277进气阀维修包02250155-971进气阀维修包88290003-548进气阀02250130-050进气阀02250088-348进气阀02250060-988进气阀88291006-018进气阀02250083-783进气阀02250045-626进气阀02250141-182进气阀02250155-970进气阀88290009-185进气阀041110进气阀88291004-492进气阀049905单向阀02250115-272单向阀02250110-557单向阀408893往复阀02250051-538电脑板88290008-999电脑板88290007-999电脑板02250106-360电脑板88290007-789电路板88320001-052控制器041006消声器040758消声器043386灯泡88290006-089红色灯泡88290006-090绿色灯泡02250049-258轴封600893-001轴封02250057-037轴封02250050-363轴封02250050-364轴封02250123-809轴封868430-030三通826502-210O型圈826502-214O型圈826502-240O型圈826502-426O型圈88842026-051O型圈88842035-047O型圈88842035-023O型圈88842035-061O型圈88842035-065O型圈88842035-075O型圈88842030-044O型圈02250099-630尼龙管02250054-861尼龙管02250052-841管芯02250099-052管芯88290001-129汽缸044912循环卸荷阀046782循环卸荷阀维修包02250046-568减压阀040691压力表02250117-009压力表250005-185压力表02250100-095温度开关02250136-694温度表02250050-154压差开关02250117-009压力表88290001-219视油镜88290004-380排气压力表250042-010计时器88290006-159温度表88290005-932阻塞指示器88290005-481安全阀88290005-480安全阀88290001-476安全阀88290005-469安全阀88290005-468安全阀88290005-471安全阀88290005-472安全阀88290005-473安全阀88290001-950安全阀88290005-479安全阀88290011-135移动机安全阀88290010-713移动机安全阀250024-654安全阀250029-330安全阀02250097-349安全阀88290001-466空滤88290001-467空滤88290002-337空滤88290002-338空滤88290003-111空滤88290001-469空滤88290004-372空滤88290007-018空滤406631联轴器弹性元件250004-641联轴器弹性元件250018-551联轴器弹性元件867308-250联轴器螺栓88290003-312联轴器弹性元件88290003-404联轴器弹性元件02250152-670联轴器弹性元件88290019-861联轴器弹性元件88290009-996联轴器弹性元件  88290019-862联轴器弹性元件250004-638联轴器02250111-222联轴器88290009-988联轴器88290004-374联轴器88290009-989联轴器88290013-848联轴器88290009-988联轴器88290009-992联轴器88290009-989联轴器88290001-904移动机联轴器88290006-808移动机联轴器橡胶88290003-397联轴器橡胶88290003-322联轴器橡胶88290003-326联轴器橡胶88290010-075联轴器橡胶88290010-079联轴器橡胶02250115-957最小压力阀蝶阀02250127-402减压阀88290017-745气水分离器88290003-393气水分离器88290001-403气水分离器88290003-109气水分离器02250111-238气水分离器88290003-209气水分离器88290004-746气水分离器88290013-186气水分离器88290020-910气水分离器88290023-106气水分离器88290017-431气水分离器88290001-040气水分离器02250127-121气水分离器 02250081-108维修包02250078-841气水分离器88290010-023电子排水阀88290010-699电子排水阀88290012-751电子排水阀88290010-561电子排水阀88290012-684电子排水阀88290012-779电子排水阀配件88290012-780电子排水阀定时器02250081-108水分离器维修包02250087-422水分离器维修包044374水分离器维修包250004-642压缩机端连接毂250018-005电机端连接毂02250125-775节流孔02250098-303球阀250039-909温度传感器02250058-087温度传感器02250048-457温度传感器001104移动机温度开关250039-910传感器88290003-806传感器88290004-947风冷组合冷却器88290005-380风冷冷却器88290005-200风冷冷却器88291001-504风冷冷却器88290017-243风冷冷却器88290020-670冷却器88290004-083水冷油冷却器88290003-414冷却器88290003-413冷却器88290001-549冷却器88290002-198冷却器88290006-633冷却器88290003-019冷却器88290002-536冷却器88290001-549冷却器88290002-817水冷油冷却器88290002-818冷却器02250096-706风冷冷却器88290002-457冷却器88290002-458冷却器88290003-120风冷冷却器88290017-242风冷冷却器88290006-640风冷冷却器88290012-760风冷油气冷却器总成88290012-803风冷油冷却器88290007-938风冷后冷却器88290006-982风冷空油冷却器88290001-357水冷油冷却器88290001-358水冷后冷却器88290009-786水冷冷却器88290006-636冷却器88290006-635冷却器88290003-025水冷油冷却器88290003-026水冷后冷却器88290004-345水冷后冷却器02250111-201水冷后冷却器02250111-202水冷油冷却器88290014-166油冷却器88290013-028后冷却器88290004-346水冷油却器88290006-645后冷却器88290006-644油冷却器88290004-532冷却器88290004-533油冷却器88290004-534空气冷却器88290006-382不锈钢水冷油冷88290003-114油冷却器88290006-643风冷空油冷却器88290009-734风冷冷却器88290006-634风冷空油冷却器02250094-744水冷油冷却器250017-527水冷后冷却器040680水冷后冷却器042950水冷后冷却器88290005-930水冷油冷却器88290020-662水冷后冷却器88290020-661水冷油冷却器88290004-347水冷后冷却器88290020-663水冷油冷却器88290014-478风冷冷却器88290015-765风冷冷却器88290014-225风冷冷却器88291002-894-00风冷冷却器88291007-378风冷冷却器88290012-331风冷冷却器88290013-517风冷冷却器88290013-267风冷冷却器88290013-518风冷冷却器88290013-269风冷冷却器88290013-519风冷冷却器88291006-08888291006-99588290007-939冷却器02250150-059移动机冷却器02250124-822移动机冷却器02250166-257移动机冷却器88290002-664移动机油冷88290001-901移动机发动机冷却器88290019-198水冷后冷却器88290019-197水冷油冷却器040550橡胶弯头88290004-441金属软管88290002-396金属软管88290002-392金属软管88290001-396金属软管02250097-827金属软管88290006-985金属软管88290006-983金属软管88290013-020金属软管02250110-538金属软管88290001-579金属软管88290001-398金属软管88290019-517金属软管88290006-685金属软管88290001-895金属软管88290001-893金属软管88290003-567金属软管88290005-550金属软管88290004-444软管88290004-445软管88290004-446软管88290004-646软管02250127-905软管88290017-244软管02250098-622软管02250098-624软管02250143-476软管02250141-104软管88290009-266软管88290009-268软管88290009-267软管88290001-425软管88290001-426软管88290005-227软管88290005-228软管88290002-328软管88290002-330软管88290002-331放油软管88290002-332软管88290003-395软管88290005-540软管88290005-541软管88290005-542软管88290005-545软管040413回油软管88290015-902皮带88290001-042橡胶软管249608-021移动机软管02250054-260移动机软管88290006-685移动机软管88290012-445进气软管049625进气软管020169接头02250101-490接头883727-012接头883527-012接头250016-351启动按钮250003-798油分离器压差表250003-799油过滤器压差表040029加油盖88290006-366视油镜046559视油镜88290002-666风扇电机88290007-878风扇电机88290001-755风扇电机88290009-879风扇88290004-940电动机88290002-576电动机250003-797空滤器真空表250022-632高压停机开关250034-304传动轴250034-305传动轴02250077-596传动轴02250094-411齿轮02250094-735齿轮499097-188齿轮499096-188齿轮490054-206齿轮490055-206齿轮145-25800齿轮145-25700齿轮250039-253排水器88290001-732时间继电器88320001-035压力开关041076止逆阀88290002-649绿色按钮88290002-651红色按钮88290015-538急停按扭02250049-934油滤底座040136球绞接头02250084-094温度开关250041-638机油压力开关88290012-827机油压力传感器88290012-829机油温度传感器88290010-831张紧轮轴88290010-830张紧轮042148压差指示器88290003-022减震垫88290007-005油气分离器桶88290014-226油气分离器桶88290006-100压力开关88290001-302风扇热继电器88290015-740单向交流电压数显表 

该公司产品分类： 空压机配件

光功率计 OPM521100A B C [850nm-1625nm] FC

光功率计 OPM521100A B C [850nm-1625nm] FC 特征：·FC, SC, ST 适配器和2.5mm UPP·数据存储

·数据传输

·实时测试的数据可同步于电脑

·内置VFL

·省电模式

·参考值存储

·850/1300/1310/1490/1550/1625nm

·可连续工作100小时

·一年保修，三年免费建议校准

技术参数：

 

型号

OPM521100A

OPM521100B

OPM521100C

测量范围

+10~ -70

+18~ -60

+26~ -50

精确度

± 0.2 dB

校准波长

850nm / 1300nm / 1310nm / 1490nm / 1550nm / 1625nm

分辨率

0.01dB

接口

FC  & 2.5mm UPP, (选项: SC,ST)

参考值存储

有

自动关机

10分钟后无操作(可取消)，低电量

内置 VFL (可选)

1mW或者10mW或者自定义

数据存储

40痕迹

电池充电

有

波号 (可选)

有

数据传输(可选)

有

实时监控器(可选)

10分钟到360天,间隔:0.5s, 2s, 15s, 60s, 180s, 600s

电池寿命

100小时以上

电池供应

AA * 2或交流适配器

规格

—

尺寸（长*宽*高）

170mm * 97mm * 38mm

重量

约380g

存储温度

-20 －－ +60 ℃, < 90%RH

使用温度

-10 －－ +50 ℃, < 90%RH

标准配置清单

—

标准

可选

校准证书*1

交流适配器*1

说明书*1

SC互换适配器*1

便携包*1

ST互换适配器*1

硬件和软件的个人电脑连接器*1
http://www.yach.com/Instrument.html   更多光学仪器仪表

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德国沃申道夫WDG-C8B1B-0013-C100-CRW　

德国沃申道夫WACHENDORFF编码器     德国Wachendorff（沃申道夫）品牌由Rolf Wachendorff（沃申道夫） 于1978年11月17日在德国的Geisenheim创建，至今已有20多年的发展进程。德国Wachendorff（沃申道夫）自动化公司为客户提供坚固的工业元件及智能化系统，专用在高精度的测量位移、角度及方向的工业场合中。坚固的工业级Wachendorff（沃申道夫）编码器的正常质保期为五年，远远高于欧洲大部分品牌的一年质保期的期限。     其他工业电子产品。     具体产品有：     Opus 系列　 OPUS A1 , OPUS A2 , OPUS Thor ,OPUS 21     WDG　 系列　 WDG 58B , WDG SSI , WDG 40A , WDG 80H , WDG 58V , WDG 58K, WDG 115T , WDG 115M 等     WDG 58B-1500-ABN-G24-K3　　　　 WDG 40A-500-ABN-I24-K2     WDG 53S-5000-ABN-I24-K2　　　　 WDG 90B-1024-AB-H30-S2     WDG 115T-2048-AB-R30-S3　　　　 WDG 115M-1800-ABN-G24-S3     SZG 65-1250-N-UP-G24-05　　　　 WDG-DPB1B-0013-C100-0CC     WDG-SL00G-1213-C100-CRW　　　　 WDG-S100G-0013-B150-PRL     WDG-C8B1B-0013-C100-CRW　　　　 WDGHA1005　　　　　 WDGHA1210

 

Wachendorff（沃申道夫）公司的核心价值观一直是为控制、检测、优化工控系统设计生产销售设备。Wachendorff（沃申道夫）以产品广泛应用于恶劣的环境而赢得美誉，同时公司产品也大量应用于便携式或可移动的操作平台。 

ZARN50110-L-TV轴承FAG_ZARN50110-L-TV轴承尺寸

的专营进口轴承销售的企业之一，该公司是ZARN50110-L-TV轴承FAG授权代理商，专业销售各种进口轴承，主营品牌以瑞典SKF进口轴承轴承型号查询、德国FAG和INA、SKF轴承型号查询日本NSK、美国TIMKEN为主等国际知名品牌的进口轴承。欢迎来电咨询ZARN50110-L-TV轴承尺寸、价格、技术支持或同型号其他品牌替换等详细资料！如果您对FAGZARN50110-L-TV轴承有疑问，请联系我们获取更多ZARN50110-L-TV轴承的最新信息 FAG轴承。FAG供应商供应的产品主要用于机械制造、汽车、农机、机床轻工日本IKO轴承、冶金、矿山机械、石油化工、等各行各业之中。联系方式：电话 ： 传真： NADELLA滑块ZARN50110-L-TV轴承 ZARN50110-L-TV轴承价格 ZARN50110-L-TV轴承尺寸ZARN50110-L-TV轴承报价 ZARN50110-L-TV轴承图片NADELLA轴承 【ZARN50110-L-TV轴承】尺寸参数表：轴承型号：ZARN50110-L-TV轴承内径：50 mm 外径：110 mm 厚度：17,5 mm 品牌：FAG轴承系列：滚针/推力圆柱滚子轴承NADELLA线性模块 上海东晟轴承有限公司提供FAG轴承ZARN50110-L-TV的军工、针织机械、纺织辅助设备、气动焊机、电泳槽、等技术参数。我公司本着“ 专业放心” 是我们的服务宗旨。为客户提供快速、FAG轴承便捷、优质、专业的轴承型号查询服务。我公司愿与您在平等互利的基础上真诚合作共图发展大计。推荐型号：NTNNUP2216轴承  NUP2217  NTN NUP2218E轴承  NTN NUP2218  NTNNUP2219E  NTNNUP2219轴承  NUP2220E  14118/14276-B轴承  26118-S/26283-B  3190/3120-B轴承  17119/17244-B  M86649/M86611-B轴承  2558/2523-B  3187/3120-B轴承  3191/3120-B  62302.2RSR  NU2204R轴承  639/632-B  61801-RS轴承  22352RHAKW33  6056M轴承  IKO轴承  德国NADELLA滑块 公司网站：http://www.bearings365.comhttp://www.zcsearch.comhttp://www.sh-bearings.comhttp://www.zhoucheng188.comhttp://www.ikont.com.cnhttp://www.shdszc.com参考资料：http://www.zhoucheng188.com/FAG/ZARN50110-L-TV.html

 

 

该公司产品分类： 轴承

CSB-EL011580RA大鼠白介素10(IL-10)ELISA KIT

 Rat Interleukin 10（IL-10）  

ELISA Kit

 

 

 

Catalog No. CSB-E04595r

（96T）

 

 

 

 

 

l          This immunoassay kit allows for the in vitro quantitative determination of rat IL-10 concentrations in serum, plasma ａnd Tissue Homogenates.

l          Expiration date   six months from the date of manufacture

l          FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

 

 

PRINCIPLE OF THE ASSAY

The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-10 and Avidin conjugated to Horseradish Peroxidase （HRP） is added to each microplate well ａnd incubated. Then a TMB （3,3＇,5,5＇ tetramethyl-benzidine） substrate solution is added to each well. Only those wells that contain IL-10, biotin-conjugated antibody ａnd enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution ａnd the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

DETECTION RANGE

3.12 pg/ml-200 pg/ml. The standard curve concentrations used for the ELISA’s were 200 pg/ml, 100 pg/ml, 50 pg/ml, 25 pg/ml, 12.5 pg/ml, 6.25 pg/ml, 3.12 pg/ml.

SPECIFICITY

This assay recognizes rat IL-10. No significant cross-reactivity or interference was observed.

SENSITIVITY

The minimum detectable dose of rat IL-10 is typically less than 0.78 pg/ml.

The sensitivity of this assay, or Lower Limit of Detection （LLD） was defined as the lowest protein concentration that could be differentiated from zero.

MATERIALS PROVIDED

Reagent 

Quantity

Assay plate

1

Standard

2

Sample Diluent 

1 x 20 ml

Biotin-antibody Diluent 

1 x 10 ml

HRP-avidin Diluent  

1 x 10 ml

Biotin-antibody 

1 x 120μl

HRP-avidin

1 x 120μl

Wash Buffer  

1 x 20 ml

 （25×concentrate）

TMB Substrate 

1 x 10 ml

Stop Solution 

1 x 10 ml

STORAGE

1.      Unopened test kits should be stored at 2-8°C upon receipt ａnd the microtiter plate should be kept in a sealed bag. The test kit may be used throughout the expiration date of the kit, provided it is stored as prescribed above. Refer to the package label for the expiration date.

2.      Opened test plate should be stored at 2-8°C in the aluminum foil bag with desiccants to minimize exposure to damp air. The kits will remain stable until the expiring date shown, provided it is stored as prescribed above. 

3.      A microtiter plate reader with a bandwidth of 10 nm or less ａnd an optical density range of 0-3 OD or greater at 450nm wavelength is acceptable for use in absorbance measurement.

REAGENT PREPARATION

Bring all reagents to room temperature before use.

1.        Wash Buffer  If crystals have formed in the concentrate, warm up to room temperature ａnd mix gently until the crystals have completely dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to prepare 500 ml of Wash Buffer.

2.        Standard  Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 ml of Sample Diluent. This reconstitution produces a stock solution of 200 pg/ml. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making serial dilutions. The undiluted standard serves as the high standard （200 pg/ml）. The Sample Diluent serves as the zero standard （0 pg/ml）. Prepare fresh for each assay. Use within 4 hours ａnd discard after use.

3.        Biotin-antibody  Centrifuge the vial before opening. Dilute to the working concentration using Biotin-antibody Diluent（1:100）, respectively.

4.        HRP-avidin  Centrifuge the vial before opening. Dilute to the working concentration using HRP-avidin Diluent（1:100）, respectively.

Precaution: The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, ａnd clothing protection when using this material.

OTHER SUPPLIES REQUIRED

—          Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

—          Pipettes ａnd pipette tips.

—          Deionized or distilled water.

—          Squirt bottle, manifold dispenser, or automated microplate washer.

—          An incubator which can provide stable incubation conditions up to 37°C±0.5°C.

SAMPLE COLLECTION AND STORAGE

l          Serum  Use a serum separator tube （SST） ａnd allow samples to clot for 30 minutes before centrifugation for 15 minutes at 1000 g. Remove serum ａnd assay immediately or aliquot and store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

l          Plasma  Collect plasma using citrate, EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 g within 30 minutes of collection. Assay immediately or aliquot ａnd store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

l          Tissue Homogenates   100mg tissue was rinsed with 1X PBS, homogenized in 1 mL of 1X PBS ａnd stored overnight at -20° C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. The supernate was assayed ａnd removed immediately. Alternatively, aliquot ａnd store samples at -20°C or -80℃. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

Note: Grossly hemolyzed samples are not suitable for use in this assay.

 

 

 

ASSAY PROCEDURE

Bring all reagents ａnd samples to room temperature before use. It is recommended that all samples, standards, ａnd controls be assayed in duplicate. All the reagents should be added directly to the liquid level in the well. The pipette should avoid contacting the inner wall of the well.

1.        Add 100μl of Standard, Blank, or Sample per well. Cover with the adhesive strip. Incubate for 2 hours at 37°C.

2.        Remove the liquid of each well, don’t wash.

3.        Add 100μl of Biotin-antibody working solution to each well. Incubate for 1 hour at 37°C. Biotin-antibody working solution may appear cloudy. Warm up to room temperature ａnd mix gently until solution appears uniform.

4.        Aspirate each well ａnd wash, repeating the process three times for a total of three washes. Wash: Fill each well with Wash Buffer （200μl） ａnd let it stand for 2 minutes, then remove the liquid by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel. Complete removal of liquid at each step is essential to good performance.

5.        Add 100μl of HRP-avidin working solution to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.

6.        Repeat the aspiration ａnd wash five times as step 4.

7.        Add 90μl of TMB Substrate to each well. Incubate for 10-30 minutes at 37°C. Keeping the plate away from drafts ａnd other temperature fluctuations in the dark.

8.        Add 50μl of Stop Solution to each well when the first four wells containing the highest concentration of standards develop obvious blue color. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.

9.        Determine the optical density of each well within 30 minutes, using a microplate reader set to 450 nm.

CALCULATION OF RESULTS

Using the professional soft "Curve Exert 1.3" to make a standard curve is recommended, which can be downloaded from our web.

Average the duplicate readings for each standard, control, ａnd sample ａnd subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic （4-PL） curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis ａnd draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL-10 concentrations versus the log of the O.D. ａnd the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

LIMITATIONS OF THE PROCEDURE

—          The kit should not be used beyond the expiration date on the kit label.

—          Do not mix or substitute reagents with those from other lots or sources.

—          It is important that the Standard Diluent selected for the standard curve be consistent with the samples being assayed.

—          If samples generate values higher than the highest standard, dilute the samples with the appropriate Standard Diluent ａnd repeat the assay.

—          Any variation in Standard Diluent, operator, pipetting technique, washing technique, incubation time or temperature, ａnd kit age can cause variation in binding.

 

—          This assay is designed to eliminate interference by soluble receptors, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

TECHNICAL HINTS

—          Centrifuge vials before opening to collect contents.

—          When mixing or reconstituting protein solutions, always avoid foaming.

—          To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, ａnd between reagent additions. Also, use separate reservoirs for each reagent.

—          When using an automated plate washer, adding a 30 second soak period following the addition of wash buffer, and/or rotating the plate 180 degrees between wash steps may improve assay precision.

—          To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary.

—          Substrate Solution should remain colorless or light blue until added to the plate. Keep Substrate Solution protected from light. Substrate Solution should change from colorless or light blue to gradations of blue.

—          Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution. Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

 

 

该公司产品分类： 科研试剂

KEM自动滴定仪AT-710系列

KEM自动滴定仪AT-710系列KEM自动滴定仪AT-710系列主要特点:AT-710M:触摸屏和操作单元之间无线连接，同时控制四台自动滴定测量单元。通过使用无线适配器(蓝牙)，操作单元和滴定装置可以不用电缆连接。在测量样品过程中有危害性气体时，可将滴定装置放置在排烟柜中，由无线传输的操作单元执行滴定。AT-710M/AT-710S:滴定剂的信息存储在滴定管上的芯片中。即使滴定管单元转移到另一个滴定装置,也不需要重新输入滴定剂信息。AT-710M/AT-710S/AT-710B:滴定管单元特殊的开关阀门。减少滴定管和开关阀门间的死体积。特殊结构的设计可减少滴定剂的使用量和快速更换滴定单元。AT-710M/AT-710S/AT-710B:智能化电极电缆。电极的信息可透过电缆上传感器存储相关资料。电极校准的数据可应用到不同的自动滴定仪上。KEM自动滴定仪AT-710系列技术参数:

名称

自动电位滴定仪

仪器型号

AT-710M

AT-710S

AT-710B

仪器组成

MCU-710M+AT-710+螺旋桨或磁力搅拌器

MCU-710S+AT-710+螺旋桨或磁力搅拌器

AT-710+螺旋桨或磁力搅拌器

测量范围

1)电位: -2000.0～2000.0mV2) pH: -20.000～20.000pH3)温度: 0～100°C

滴定方式

自动滴定，自动间歇滴定，间歇滴定，恒pH滴定

石油中和值滴定，COD滴定

滴定类型

电位滴定(酸碱，氧化还原，沉淀)，光度滴定，极化滴定，电导滴定

终点判断

全量滴定(自动终点)，自动终点滴定，设定终点滴定

交叉点滴定，自动终点/设定终点滴定

特殊应用

量电极电位(pH, mV)，酸解离常数(pKa)

同时记录双通道电位，学习滴定

输入设置

触摸屏输入

按键输入

显示

1) 8.4英寸彩色液晶屏，800x600点

1) LED背景光源液晶屏

2)英文/日文/中文/韩文/俄文/西语/德语/法语

2)英文/日文/中文/韩文/俄文/西语

3)四个通道同时显示

3)一个通道显示

3)一个通道显示

计算

浓度计算，统计计算(平均值，标准差，相对标准差)，自动输入空白和滴定度

数据储存

500组样品结果

50组样品结果

GLP认证

登记操作者/使用群组管理滴定剂:提示滴定度测量日期/指示滴定剂剩余量/提示滴定管活塞更换日期/提示滴定剂换日期/滴定度测量履历性能检查:提醒计划检查日期/记录检查结果电极管理:记录校正日期/记录校正履历/电极检查/电极检查履历滴定管验证:验证/记录验证结果时间管理:显示操作时间

登记操作者/记录检查结果/电极校正记录/滴定管精度确认/时间管理

滴定管单元

20mL玻璃滴定管附褐色保护套(标配)，选配: 10mL，5mL或1mL

滴定管精确度

50mL滴定管(自动注入器): &plusmn;0.5mL20mL滴定管: 0.02mL         重复性: 0.01mL10mL滴定管: 0.015mL       重复性: 0.005mL5mL滴定管: 0.01mL           重复性: 0.003mL1mL滴定管: 0.005mL         重复性: 0.001mL

扩大器

1) STD: pH(mV), mV，双通道(标配)2) PTA: pH(mV), mV，光度,三通道3) POA: pH(mV), mV，极化,三通道4) CMT: pH(mV), mV，电导,三通道5) TET: pH(mV)，pH(mv), mV，三通道

外部输出

RS-232C x3

RS-232C x 2

打印机，电子天平，数据软件(SOFT-CAP)

USB x; 1

USB x 1

U盘，热敏打印机，A4打印机，键盘，            条码机，脚踏开关, USB集线器

U盘，热敏打印机，键盘，条码机，脚踏开关, USB集线器，安卓设备

LAN x 1 :电脑(PC)

扩充功能

测量单元:电位滴定仪(AT-710)容量水分仪(MKV-710)库仑水分仪MKC-710)最多四台测量单元

自动活塞滴定管:最多可控制10台滴定管驱动单元(包括主机两台)

多样品自动进样器: CHA-600，CHA-700

CHA-700

使用环境

1)温度: 5～35°C2)相对湿度: 85%RH以下

电源

AC100～240V，50Hz/60Hz

耗电量

主机:约30瓦打印机:约7瓦

主机:约20瓦打印机:约7瓦

尺寸

触摸屏: 225(W) x 190(D) x 42(H)mm

滴定单元: 141(W) x 296(D) x 367(H)mm(不包括管路)打印机: 106(W) x 180(D) x 88(H)mm

重量

触摸屏:约1.5公斤

滴定单元:约4.0公斤打印机:约0.4公斤

 

该公司产品分类： 电磁旋转粘度计 热分析相关仪器 石油产品专用仪器 食品饮料专用仪器 热流计、热流仪 导热仪、热导仪 糖度计、糖度仪 折光仪、折射计 密度计、密度仪 卡氏水分测定仪 自动电位滴定仪

QKT45-100/250专业生产供应【QKT45-100/250司机控制器】质量保证价格合理

【QKT45-100/250司机控制器】原型号QKT45-3QKT45-100/250矿用一般型司机控制器   QKT45-3矿用一般型司机控制器 QKT45-100/250司机控制器适用范围及工作条件：QKT45-100/250矿用一般型司机控制器（老型号QKT45-3）用于5T/7T架线式电阻调速机车，作为机车起动调速、停车、反向之用。本司机控制器适用于下列工作环境：1、海拔不超过1200M（超过时与用户协商）。2、环境温度为-20℃-+40℃（月平均温度为+25℃时）。3、周围空气相对湿度不超过90%。4、无可燃性气体和煤尘爆炸混合物的场所。5、无足以破坏绝缘和腐蚀金属的有害气体。6、有防雨、防雪、防溅保护的装置上。7、相对于正常位置倾斜在10度以内的情况下8、相对于机车的纵向具有不大于30m/s2的冲击加速度。9、相对于机车的纵向、横向和垂直方向具有频率f为2-50HZ的正弦振动，其振幅A不大于:2≤f≤10HZ       A=25/f（mm）10≤f≤50HZ     A=250/f2（mm）10、安装于XK5T或XK7T蓄电池式矿用电机车司机控制器室内。QKT45-100/250司机控制器技术参数：1、额定电压：250V（DC）；2、额定电流：100A；3、触头其接触面不少于接触方向全长的80%。4、触头与触头对齐相差不大于1mm。5、触头开距＞8mm。6、触头初压力＞14.7N 7、触头终压力＞29.4N  济宁市卓信工矿设备有限公司秉承山东人的豪爽与实诚，诚邀各位专家、领导来我公司考察、指导、赐教并期盼与您真诚合作，“追求卓越”永远是我们的主题，我们将励精图治，再创高效，力争以先进的技术，优良的产品质量，为煤矿企业提供多方位的服务。手机：18764769169

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网站：http://www.jnzhuoxin.com

该公司产品分类： 电机车 电机车配件 电泵风泵 凿岩机械

DC-2010低温恒温槽

 本公司系列低温恒温槽采用国际最新先进无氟制冷系统。广泛用于石油、化工、电子仪表、物理、化学、生物工程、医药卫生、生命科学、轻工食品、物性测试及化学分析等研究部门，高等院校，企业质检及生产部门，为用户工作时提供一个冷热受控，温度均匀恒定的液体环境，对试验样品或生产的产品进行恒定温度试验或测试，也可作为直接加热或制冷和辅助加热或制冷的热源或冷源

规格: (1套)

 

 

温度范围：

-20~100℃

 

温度波动度：

±0.05℃

 

数显分辨率：

0.1/0.01

 

工作槽容积：

250×200×200（mm）

 

槽深度：

200mm

 

循环泵流量：

6L/min

 

工作槽开口：

180×140(mm)

 

排水口：

有

 

 

 

 

 

 

 

 
   

 

 

 

 

 

该公司产品分类： 溶出度仪、溶出仪、溶出试验仪 手消毒器 金属浴/恒温金属浴/干式恒温器 细胞计数器/细胞计数仪 其它常用设备 微生物检测系统/微生物快速检测系统 水浴锅、恒温水浴锅 制冰机、雪花制冰机 细胞融合仪 超声波探伤仪 研磨机、研磨仪、粉碎机、球磨机 冻干机、冷冻干燥机 其它动物实验仪器 核酸分子杂交仪 基因导入仪、细胞融合仪 分散机 匀浆机 存储 均质器 、均质机、乳化机 洗瓶机/清洗机

FM910FM910电源模块

供应和利时模块QQ:642706330   供应和利时模块 型号齐全,欢迎来电咨询!网址: www.zbxinshuo点击与二号售前客服交谈客服[1]（QQ 389924996）    点击与四号售前客服交谈 客服[4]（QQ 642706330）FM131A FM138-DCC FM138-DCD FM138-SSR FM141 FM142 FM143 FM143A FM143G FM144A FM145B FM146A FM1305-A FM147A FM148 FM148C FM148E FM148R FM151A FM131AR-AI-I FM131-C FM131-D FM131-E-A FM131-DEH FM131A-DEH-B FM132 FM133 FM134 FM136 FM138-ACC FM138-ACD FM151R FM152A FM161D FM161D-SOE FM161D-48 FM161D-48-SOE FM161E-48-SOE FM162 FM163A FM165 FM171 FM171B FM181 FM182 FM182A FM191 FM191A FM191A-A FM192A-CC FM192B-CC FM192A-TR FM197 FM811-K01 FM811-SP-K01 FM801 FM803 FM300-A FM301 FM305-A FM910 FM920 FM931 FB121 FB136 FB137 FB138-DC-B FB138-AC-B FB138-SSR-C FB139 FB149-D FB193 FB194 FB196 FB197 FM111 FM111R FM112 FM112R FM113（计算机；SSC5X86H/32M EDO/8MB DOC） FM113R FM114 FM1200 FM1201 FM1202-B FM1303 PE1800 GX280 GX520nSMT

100X遥控浮球阀 遥控浮球阀 浮球阀 水力控制阀

工作原理：    F100X-遥控浮球阀介质由导管传送给导阀(小浮球阀),当水池(箱)水位较低时,导阀开启,主阀开始向水池(箱)供水,当浮球随水位上升至所控制的最高水位时,导阀关闭,主阀也随之关闭,停止向水池供水。工作特点:     控制水塔或水池的液面，保养简介,灵活耐用,液位控制准确度高,水位不受水压干扰且关闭紧密不漏水。控制浮球可与主阀体分离式安装。安装要求：  1. 安装前,应将管道冲洗干净,并加过滤器。  2. 浮球及导阀安装位置应便于观察,远高主阀进水口或另设小水箱,防止水面波动使导阀错误动作,导致主阀不停的开关。调试：     在运行状态下,针阀用于控制主阀的关闭速度,通常保持1/2至2圈开度较合适,不宜过大,以减少主阀“猎振”现象,主阀上其它手动阀门应全开。F100X-遥控浮球阀简图  安装示意图: 安装示意图 
DN 50 65 80 100 125 150 200 250 300 350 400
L 230 240 285 325 360 400 470 575 640 700 740
H 225 300 320 420 450 500 710 770 905 970 1000
泊头盛丰自控阀门有限公司http://www.sfzkfm.com
该公司产品分类： 暖通阀 防污阀 排气阀 过滤器 软密封闸阀 水力控制阀 超声波热量表 超声波流量计 远传水表 智能卡式水表
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