深圳嘉典盛自动化有限公司主要产品为可程式控制器(PLC)、远端输出/输入模组(RTU)、工业电脑(IPC).经营品牌:三菱、欧姆龙,艾默生
Synonyms | Listeriolysin-O, LLO, hlyA. |
Introduction | Listeriolysin O (aka LLO) is a hemolysin produced by Listeria monocytogenes bacteria, the pathogen responsible for causing listeriosis. The toxin may be regarded as a virulence factor, since it is crucial for the virulence of L. monocytogenes. LLO is a single polypeptide protein encoded by the hlyA gene and composed of 529 residues. LLO is a thiol-activated cholesterol-dependent pore forming toxin protein; therefore, it is activated by reducing agents and inhibited by oxidizing agents. Still, LLO differs from other thiol-activated toxins, as its cytolytic activity is maximized at a pH of 5.5. Inside the acidic phagosomes (average pH ~ 5.9) of cells that have phagocytosed L. monocytogenes, LLO is selectively activated by maximizing activity at a pH of 5.5. Following the phagosome lysis by LLO, the bacterium breaks out into the cytosol, where it is able to grow intracellularly, and the toxin has reduced activity in the more basic cytosol. Thus, LLO permits L. monocytogenes to break out from the phagosomes into the cytosol without harming the plasma membrane of the infected cell, which allows the bacteria to live intracellularly, where they are sheltered from extracellular immune system factors such as the complement system and antibodies. LLO also brings about dephosphorylation of histone H3 and deacetylation of histone H4 in the early phases of infection, before entry of L. monocytogenes into the host cell. The pore-forming activity is not implicated in causing the histone modifications. The modifications of the histones affect the down regulation of genes encoding proteins involved in the inflammatory response. Therefore, LLO may be significant in subverting the host immune response to L. monocytogenes. At its NH2-terminus it possesses a 25 residues long typical signal sequence excited during the secretion process. Moreover, in its NH2-terminus there is also a 19 amino acids PEST- like sequence that may target this toxin for degradation. The PEST-like sequence found in LLO and is considered crucial for virulence, given that mutants lacking the sequence lysed the host cell. Nevertheless, contrary to PEST's supposed role in protein degradation, evidence implies that the PEST-like sequence may control LLO production in the cytosol rather than increase degradation of LLO. |
Description | Molecular weight of Listeriolysin O is 58kDa. This haemolysin protein is provided in its mature form that is constituted by 503 amino acids, from 26 to 529 of the registered sequence (accession number: AAF64524). |
Source | Escherichia Coli. |
Physical Appearance | Sterile Filtered clear solution. |
Formulation | The protein contains 50mM NaH2PO4, 1mM EDTA, 2.7mM KCl, 1mM DTT, 5% glycerol and 0.5M NaCl. |
Stability | LLO although stable at room temperature for 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles. |
Purity | Greater than 90.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Activity | LLO has a hemolytic activity 1.25x106 HU/mg. 2mM DTT is used to reactivate the toxin. |
Applications | a. Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA (4). b. Production of specific monoclonal antibodies (5). c. Detection of anti-listeriolysin O antibodies (6). |
Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
型号 | 仪器尺寸(mm) | 清洗槽内尺寸(mm) | 容量 | 超声频率 | 超声功率 | 加热功率 | 时间可调 |
| 长×宽×高 | 长×宽×高 | L | (KH) | (W) | (W) | (min) |
KQ116 | 223×114×123 | 167×90×52 | 0.6 | 40 | 50 | 无 | 10 |
KQ118 | 223×114×123 | 167×90×52 | 0.6 | 40 | 70 | 无 | 10 |
KQ218 | 223×114×123 | 167×90×52 | 0.6 | 40 | 100 | 无 | 10 |
KQ-50 | ф145×210 | ф140×100 | 1.5 | 40 | 50 | 无 | 无 |
KQ-50B | 170×160×210 | 150×140×100 | 2 | 40 | 50 | 无 | 1-20 |
KQ-50E | 208×192×210 | 150×140×100 | 2 | 40 | 50 | 100 | 1-20 |
KQ2200 | 260×160×260 | 230×140×100 | 3 | 40 | 100 | 无 | 1-20 |
KQ2200B | 260×160×260 | 230×140×100 | 3 | 40 | 100 | 200 | 1-20 |
KQ2200E | 260×160×280 | 230×140×100 | 3 | 40 | 100 | 200 | 1-20 |
KQ2200V | 340×250×330 | 230×140×130 | 4 | 40 | 100 | 200 | 1-20 |
KQ-100 | 320×174×260 | 300×150×100 | 4 | 40 | 100 | 无 | 1-20 |
KQ-100B | 320×174×260 | 300×150×100 | 4 | 40 | 100 | 400 | 1-20 |
KQ-100E | 320×174×280 | 300×150×100 | 4 | 40 | 100 | 400 | 1-20 |
KQ-100V | 410×260×330 | 300×150×130 | 5.8 | 40 | 100 | 400 | 1-20 |
KQ3200 | 320×174×320 | 300×150×150 | 6 | 40 | 150 | 无 | 1-20 |
KQ3200B | 320×174×320 | 300×150×150 | 6 | 40 | 150 | 400 | 1-20 |
KQ3200E | 320×264×340 | 300×150×150 | 6 | 40 | 150 | 400 | 1-20 |
KQ3200V | 410×260×380 | 300×150×180 | 8 | 40 | 150 | 400 | 1-20 |
KQ5200 | 320×264×320 | 300×240×150 | 10 | 40 | 200 | 无 | 1-20 |
KQ5200B | 320×264×320 | 300×240×150 | 10 | 40 | 200 | 400 | 1-20 |
KQ5200E | 320×264×340 | 300×240×150 | 10 | 40 | 200 | 400 | 1-20 |
KQ5200V | 410×350×380 | 300×240×180 | 13 | 40 | 200 | 400 | 1-20 |
KQ-250 | 320×264×320 | 300×240×150 | 10 | 40 | 250 | 无 | 1-20 |
KQ-250B | 320×264×320 | 300×240×150 | 10 | 40 | 250 | 400 | 1-20 |
KQ-250E | 320×264×340 | 300×240×150 | 10 | 40 | 250 | 400 | 1-20 |
KQ-250V | 410×350×380 | 300×240×180 | 13 | 40 | 250 | 400 | 1-20 |
KQ-300 | 320×264×320 | 300×240×150 | 10 | 40 | 300 | 无 | 1-20 |
KQ-300B | 320×264×320 | 300×240×150 | 10 | 40 | 300 | 400 | 1-20 |
KQ-300E | 320×264×340 | 300×240×150 | 10 | 40 | 300 | 400 | 1-20 |
KQ-300V | 410×350×380 | 300×240×180 | 13 | 40 | 300 | 400 | 1-20 |
KQ-500 | 530×320×360 | 500×300×150 | 22.5 | 40 | 500 | 无 | 1-20 |
KQ-500B | 530×320×360 | 500×300×150 | 22.5 | 40 | 500 | 800 | 1-20 |
KQ-500E | 530×320×380 | 500×300×150 | 22.5 | 40 | 500 | 800 | 1-20 |
KQ-500V | 610×410×380 | 500×300×180 | 27 | 40 | 500 | 800 | 1-20 |
KQ-600 | 530×320×360 | 500×300×150 | 22.5 | 40 | 600 | 无 | 1-20 |
KQ-600B | 530×320×360 | 500×300×150 | 22.5 | 40 | 600 | 800 | 1-20 |
KQ-600E | 530×320×380 | 500×300×150 | 22.5 | 40 | 600 | 800 | 1-20 |
KQ-600V | 610×410×380 | 500×300×180 | 27 | 40 | 600 | 800 | 1-20 |
KQ-700 | 530×320×360 | 500×300×150 | 22.5 | 40 | 700 | 无 | 1-20 |
KQ-700B | 530×320×360 | 500×300×150 | 22.5 | 40 | 700 | 800 | 1-20 |
KQ-700E | 530×320×380 | 500×300×150 | 22.5 | 40 | 700 | 800 | 1-20 |
KQ-700V | 610×410×380 | 500×300×180 | 27 | 40 | 700 | 800 | 1-20 |
------主要特点------ |
开关选择交流输入电压范围有短路保护/过载保护/过电压保护/高温保护国际安规认证:UL/CUL90KHz固定开关频率由内置直流风扇强制通风冷却自动控制开关强制通风冷却100%满载老化测试2年质保 ------主要指标------
交流输入电压 | ..........开关选择88~132VAC/176~264VAC |
交流冲击电流 | ..........冷启动,115V时为15A/230V时为30A |
直流调整范围 | ..........额定输出电压的±10% |
过载保护 | ..........过电流点在105%~150%时断开,自动侦测,自动复原 |
过电压保护点 | ..........额定输出电压的115%~135%时动作 |
初始化、上升、保持时间 | ..........230VAC满载时2000ms,20ms,20ms |
绝缘特性 | ..........输入端与输出端间:3KVAC,输入端与外壳间:1.5KVAC1分钟 |
工作时环境温度 | ..........0~40℃可满载,-10℃时降载20%,60℃时降载40% (24V&48V 0~50℃可满载, 60℃时降载40%) |
国际安规认证 | ..........通过UL60950-1认证 |
接线方式 | ..........9P/9.5mm带盖端子排 |
机壳型号 | ..........912 |
外形尺寸 | ..........215×115×50 mm |
重量及包装 | ..........1.15公斤/只;每箱12只/15公斤/0.0283立方米 |
大量TBI螺母,滚珠丝杆,现货供应,SFI3205,型号齐全
红外测温仪GM320
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*测量范围:-50 ~ 330℃ (-58~626℉)
*精确度:±1.5% or ±1.5℃
*重复性:±1% or ±1℃
*物距比:12:1
*发射率:0.95预设
*分辨率:0.1℃/℉
*响应时间:500毫秒
*响应波长:8-14μm
*℃/℉温度单位转换
*数据保持显示
*定位激光开/关选择
*LCD背光开/关选择
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*电源:2*1.5V AAA电池
*产品净重:115.1克(含电池)
*产品尺寸:144.5*38*93厘米
*包装方式:吸塑+卡纸
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