产品介绍 |
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技术指标 |
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主要技术参数:
u 最大试验力:600KN
u 试验台离地高度:300mm
u 有效试验空间:1200mm×1200mm
u 加载方式:液压
u 控载方式:手动无极可调。(全自动加载 选配)
u 活塞行程:300mm(400mm选配)
u 试验力值精度范围:≤±1%(≤±1%选配) 节范围:0.1~45KN/s(手动无极调速)
u 载荷指示精度:0.1KN(0.1KN 选配) 加载速度调
u 变形测量分辨率:液晶数显表自动采集0.01mm (选配),变形测量范围:0.0-20mm (选配)
u 加载介质:68#抗磨液压油
u 电机功率:1.2KW ,电压:380V/220V
u 主机尺寸:1600mm×1200mm×1400mm(长×宽×高)
u 主机重量:3200Kg
电气连接: | 表面贴装/板载式 |
类型: | 磁场测量 |
特点: | 操作简单,使用方便 |
供电电源: | 12VDC |
输出: | 20±4mV/V |
精确度: | <50µV/V(磁滞) |
工作温度范围: | -40℃~+125℃ |
量程: | - |
典型应用: | 磁场强度测量,磁学成像流量计 |
YSA2系列智能型万能式断路器用于控制和保护低压配电网络。一般安装在低压配电柜中做主开关起总保护作用。其技术性能已达到了当代国际上同类型产品水平。■交流额定电流630~6300A;■短路分断能力80KA~120KA(值);■额定工作电压AC690V及以下;■具有3级和4级;■抽屉式和固定式■可倒进线安装;■多种智能控制器,提供不同功能;■执行IEC60947-2/GB14048.2的标准;
超卓牌厂家ZDLSY电动Y型疏水阀产品简介:
ZDLSY电动Y型疏水阀是根据热电厂设备疏水而专业设计生产的新一代蒸汽疏水阀,该产品接近直线的流通设计,减小冲蚀,提高寿命。阀芯、阀座采用整体硬质合金,关闭内漏,检修方便等特点,根据控制系统或调节仪表的信号,切断或开启阀门,从而实现对被控参数的使用要求。因此它广泛应用于火电厂等行业的远程控制。
技术参数:
产品口径:DN15~50;
产品压力:0.6-6.4MPa;
连接方式:螺纹、法兰;
适用介质:水、油品、气、蒸汽等;
产品材质:铸钢、不锈钢、合金钢等。
连接尺寸:
公称通径DN | 20 | 25 | 32 | 40 | 50 | 65 | 80 | 100 |
流量系数 | 5 | 8 | 12 | 17 | 29 | 43 | 70 | 110 |
额定行程mm | 16 | 25 | 40 | |||||
公称压力PN | 150LB(2.0MPa)--1500LB(25.0MPa) | |||||||
工作温度 | -445 | |||||||
信号范围 | 40-20mADC 200VAC 380VAC | |||||||
电源 | 220VAC 50HZ 380VAC 50HZ 60HZ | |||||||
允许泄漏量 | 近似0泄露 | |||||||
灵敏度 | (0.5-5%)F.S |
ZDLSY电动Y型疏水阀订货须知:一、①ZDLSY电动Y型疏水阀产品名称与型号②ZDLSY电动Y型疏水阀口径③ZDLSY电动Y型疏水阀是否带附件以便我们的为您正确选型。二、若已经由设计单位选定超卓公司的ZDLSY电动Y型疏水阀型号,请按ZDLSY电动Y型疏水阀型号直接向我司销售部订购。三、当使用的场合非常重要或环境比较复杂时,请您尽量提供设计图纸和详细参数,由我们的超卓阀门专家为您审核把关。
专业定制国内外各厂家微波消解罐内罐
已有国产,何必进口。
让微波消解罐不在高“贵”
1.内罐采用高纯实验级进口增强改性处理TFM材料或PFA材料;
2.与国产PTFE相比:除PTFE的所有优点以外,TFM还具有一些特性改进:具有更低的本底保证,高温高压下抗变形性、耐渗透性、可恢复性更好,是PTFE的两倍,外观更接近于白玉色;
3.使用温度为260℃,极限甚至可达300℃;
4.特殊研发生产工艺,保证特别厂家(如CEM)的超长罐的光洁度,可定制各厂家各规格微波消解仪内罐,配套原厂微波消解仪使用;
5.经特殊加工工艺加工,优化加工工艺,确保极低的背景值(空白值),生产厂家的加工工艺也能影响您的实验结果;
与原厂相比我们的特别之处:
1.同等优质的进口原料,价格是进口的一半甚至三分之一,并非所有的国产产品,不值得信赖,做到真正的物美价廉;
2.我们所加工的内杯能与您的仪器通用,产品实验数据与原装进口一样优秀,使用寿命更长;
3.我们提供配套CEM各种型号微波消解仪使用的内罐,有全部采用TFM材质,有盖子采用TFM材质,有盖子是透明PFA材质,盖体和垫片均为TFM材质,及全PTFE材质,供客户选择;
4.我们是一家能定制各个厂家仪器标配的内罐,同时我们承诺,产品质量与原厂一致,性价比好,可以加工CEM 各种型号如MARS5、 MARS6 、安东帕、迈尔斯通、上海屹尧、山东海能等各厂家。
我公司可以定制国内外各个厂家(如CEM、耶拿、迈尔斯通、北京祥鹄、上海新仪、山东海能、北分瑞利、上海屹尧、安东帕)的微波消解仪内罐,我公司特殊研发生产工艺保证特别厂家(如CEM MARS5、MARS6、XPRESS)的超长罐的光洁度。消解完成后,可以配套我公司的赶酸电热板进行赶酸、消解处理。
南京瑞尼克科技开发有限公司
销售部:季溪 QQ:182 6176 783
电话:025-8559 7772 手机:138 138 88374
传真:025-5899 4772 E-mail:13813888374@163.com
韩国SANG-A相阿3A接头 Y型三通接头.
PY04 PY06 PY08 SANG-A PY10 PY12 PW0604 PW0804 PW0806 PW1006 SANWOPW1008 PW1208 PW1210 PY5/32 PY3/16 PY1/4 PY5/16 PY3/8 PY1/2 PW3/16-5/32 DANHI PW1/4-5/32 PW1/4-3/16 PW5/16-5/32 SANWO PW5/16-1/4 PW3/8-1/4 DANHI PW3/8-5/16 PW1/2-5/16 PW1/2-3/8 PYJ04 PYJ06 SANG-APYJ08 PYJ10 PYJ12 PYJ5/32 PYJ3/16 PYJ1/4 PYJ5/16 PYJ3/8 PYJ1/2 PWJ0604 SANG-A PWJ0806 PWJ1008 PWJ1210 PWJ1/4-5/32 PWJ5/16-1/4 PWJ3/8-5/16 PWJ1/2-3/8 PW0403C PW0604C PW1/8C-03C PW5/32C-1/8C DANHI PW1/4C-5/32C PWJ04-03C PWJ06-04C PWJ1/8C-03C SANWO PWJ5/32C-1/8C PWJ1/4C-5/32C
【Hypersil系列液相色谱柱的参数说明】 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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温州瑞昕仪器有限公司
温州瑞昕仪器有限公司是从事工业用检测仪器、理化分析仪器、计量仪器产品的销售、服务、维修一体的公司。欢迎您的咨询订购,联系方式:
公司电话:0 5 7 7-6 6 8 8 6 0 1 7
传真:0 5 7 7-6 6 8 8 6 0 1 9
邮箱:rxin17@163.com
网址:http://www.rxin17.com/
联系人:
陈进碗 手机:1 3 7 5 8 7 6 8 8 1 3 QQ:124677072
人蛔虫IgM抗体酶联免疫分析(ELISA)
试剂盒使用说明书
本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中蛔虫IgM抗体的含量。
实验原理:
本试剂盒应用双抗原夹心法测定标本中人蛔虫IgM抗体水平。用纯化的人蛔虫IgM抗原包被微孔板,制成固相抗原,往包被抗原的微孔中依次加入蛔虫IgM抗体,再与HRP标记的蛔虫IgM抗原结合,形成抗原-抗体-酶标抗原复合物,经过洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的蛔虫IgM抗体呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中人蛔虫IgM抗体浓度。
试剂盒组成:
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样本处理及要求:
1. 血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。
2. 血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。
3. 尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。
4. 细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5. 组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
6. 标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.
7. 不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
操作步骤
1. 标准品的稀释与加样:在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀;然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀;然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀;混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉。(稀释后各孔加样量都为50μl,浓度分别为60pg/ml,40pg/ml ,20 pg/ml,10 pg/ml , 5pg/ml。
2. 加样:分别设空白孔(空白对照孔不加样品及酶标试剂,其余各步操作相同)、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl(样品最终稀释度为5倍)。加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀。
3. 温育:用封板膜封板后置37℃温育30分钟。
4. 配液:将30(48T的20倍)倍浓缩洗涤液用蒸馏水30(48T的20倍)倍稀释后备用。
5. 洗涤:小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干。
6. 加酶:每孔加入酶标试剂50μl,空白孔除外。
7. 温育:操作同3。
8. 洗涤:操作同5。
9. 显色:每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.
10. 终止:每孔加终止液50μl,终止反应(此时蓝色立转黄色)。
11. 测定:以空白空调零,450nm波长依序测量各孔的吸光度(OD值)。 测定应在加终止液后15分钟以内进行。
注意事项:
1. 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存。
2. 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果。
3. 各步加样均应使用加样器,并经常校对其性,以避免试验误差。一次加样时间控制在5分钟内,如标本数量多,推荐使用排枪加样。
4. 请每次测定的同时做标准曲线,做复孔。如标本中待测物质含量过高(样本OD值大于标准品孔孔的OD值),请先用样品稀释液稀释一定倍数(n倍)后再测定,计算时请乘以总稀释倍数(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物请避光保存。
7. 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.
8. 所有样品,洗涤液和各种废弃物都应按传染物处理。
9. 本试剂不同批号组分不得混用。
10. 如与英文说明书有异,以英文说明书为准。
计算:
以标准物的浓度为横坐标,OD值为纵坐标,
在坐标纸上绘出标准曲线,根据样品的OD
值由标准曲线查出相应的浓度;再乘以稀释
倍数;或用标准物的浓度与OD值计算出标
准曲线的直线回归方程式,将样品的OD值
代入方程式,计算出样品浓度,再乘以稀释
倍数,即为样品的实际浓度。
(此图仅供参考)
试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.92以上。
2.批内与批见应分别小于9%和15%
检测范围:
1 pg/ml -70 pg/ml
保存条件及期:
1.试剂盒保存:;2-8℃。
2.期:6个月
FOR RESEARCH USE ONLY
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Drug Names
Generic Name:Human ascariasis IgM antibody (ASC-IgM) ELISA Kit.
Purpose
This kit allows for the determination of ASC-IgM concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human ASC-IgM level in the sample,use Purified Human ASC-IgM antigen to coat microtiter plate wells, make solid-phase antigen, then add ASC-IgM to wells, Combined ASC-IgM antigen which With HRP labeled , become antigen - antibody - enzyme- antigen complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ASC-IgM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
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| (20ml×20 fold) ×1bottle | (20ml×30 fold) | |
Specimen requirements
1. serum- coagulation at room temperature 10-20 mins,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2. plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3. Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4. cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS(PH7.2-7.4), Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5. Tissue samples- After cutting samples, check the weight,add PBS(PH7.2-7.4), Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS(PH7.4), Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separately. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 60pg/ml,40pg/ml ,20 pg/ml,10 pg/ml , 5pg/ml)
2.add sample:Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1. The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2. washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3. add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4. if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).
5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6. The substrate evade the light preservation.
7. Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8. All samples, washing buffer and each kind of reject should according to infective material process.
9. Do not mix reagents with those from other lots.
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Calculate
This chart for reference only
Assay range
1 pg/ml -70 pg/ml
Storage and validity
1.Storage: 2-8℃.
2.validity: six months.