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多参数水质分析仪:产品描述技术参数
技术参数:
重复性:≤2%
选配件:大功率电池 200元/块 专用铝合金箱子 150元/个 (配置后可携带至现场检测)
包装参数多参数水质分析仪:技术文档北京柏达仪和科技有限公司成立于2009年3月30日,主要从事工业产品,仪器仪表的研发,生产,进口代理与销售业务。公司的核心竞争力在于打造专业和完善的互联网营销平台,通过庞大的网络产品库和专业的服务团队,为用户量身打造适合的产品解决方案。注重客户的感受,是我们的服务理念和宗旨。作为众多知名品牌的合作伙伴,柏达仪和以其优良的品质和服务来阁下员工的职业健康,安全环境和美好未来。
豚鼠内皮素1(ET-1)ELISA Kit
guinea pig Endothelin 1,ET-1 ELISA Kit
用途: For research use only. Not for diagnostic use. 原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EDN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EDN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EDN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EDN1 bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性: This assay has high sensitivity and excellent specificity for detection of Guinea pig EDN1. No significant cross-reactivity or interference between Guinea pig EDN1 and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the EDN1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
规格参数:
容积:1加仑=3.75升
内径:136 mm
壁厚:3 mm
底厚:5 mm
高度:360 mm
宽度:180 mm
可承受气压范围:1~10 bar
另:可根据客户的具体要求订做非标压力桶。
上海理亨工贸有限公司长期代理销售西门子模块所使用的风机,种类齐全,备有库存。
上海理亨公司是德国依必安派特(ebmpapst)公司正式EBM风机产品代理商.
长期经销全部型号的ebm-papst风机其中几十种常用型号均备有充足库存现
货供应且价格优惠.
型号:
EBM R6D500-AK03-01
EBM R6D560-AH05-01
EBM R6D630-A703-01
EBM后向离心风机
EBM R4E400-AB23-05 叶轮直径为400mm的离心风机,德国原装进口,库存充
足,价格优惠!
EBM R4E310-AP11-01 FFU上一款常用风机,库存充足,价格优惠!
EBM R2E220-AA40-05
EBM R2E190-AO26-05 西门子变频器常用的一款风机,是R2E190-AE77-B8
的替代型号
EBM R4E400-AB23-05 叶轮直径为400mm的离心风机,德国原装进口,库存充
足,价格优惠!
EBM R4E310-AP11-01 FFU上一款常用风机,库存充足,价格优惠!
EBM R2E220-AA40-05
EBM R2E190-AO26-05 西门子变频器常用的一款风机,是R2E190-AE77-B8
的替代型号
EBM R4D400-AB04-05 变频器行业常用的一款离心风机!
EBM R2E225-BD92-09 叶轮直径为225mm的离心风机,库存充足,价格优惠!
EBM 轴流风机
EBM S4E350-AP06-30 外带金属网罩,扇叶直径为350mm的轴流风机
EBM S4D300-AP28-30 外带金属网罩,扇叶直径为300mm的轴流风机
EBM S4E300-AP26-30 扇叶直径为300mm的轴流风机,外带金属网罩.
EBM W2E143-AB09-01 扇叶直径143mm轴流风机.现货,价优!
EBM W2E142-BB01-01 扇叶直径142mm的金属外框轴流风机. PAPST型号为
7855ES
EBM W2E200-HK38-01 常用的一款轴流风机.我公司库存充足,价格优惠!!
EBM W2E250-HL06-01 扇叶直径250mm的轴流风机,库存充足,价格优惠!
EBM W2S130-AA03-01 扇叶直径130,金属外框
EBM 前向离心风机
EBM D2E146-AP47-22 金属蜗壳,出风口带法兰边,叶轮直径为146mm的前向
离心风机
EBM D2E146-HT67-01 空调 净化行业常用的前向离心风机,库存充足,价格优
惠!
EBM D2E146-HR93-01 叶轮直径为146mm的前向离心风机.塑料蜗壳,双进风
口.
EBM D2E133-AM47-01 金属蜗壳的前向离心风机
EBM G2E180-EH03-01 金属蜗壳的单进口前向离心风机
EBM D2E140-HR97-05 塑料蜗壳的双进口前向离心风机.
型号如下:
K2E190-AB77-09
K2E220-AB06-09
K2E225-AA26-09
K2E250-AA01-09
R2D180-AL10-18
R2D220-AB02-10
K2E225-AB92-09
D2E160-AH01-17
D2E146-AA03-43
DV6224/2-532
6224N-269
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特征:◆高压力(~100公斤)、率(85%)、低电耗(能转换)◆适用于输送含杂质浆液 油室与泵室被膜片隔开,驱动装置与浆液无接触,可以稳定运转◆低脉动、低噪音 多柱塞构造实现高速运转,控制脉动并保持低噪音◆易操作易维护 机械传动部无故障运转,膜片易于更换和维护用途◆高压喷雾造粒干燥设备:适用于染料、颜料、瓷粉、化肥、洗衣粉、奶粉、药粉等制取◆高压冲洗设备:精密加工零件、印刷电路板、船舱及生产场地等◆高压移送:切削液、粘性液体、回收水、腐蚀性液体、研磨浆料等 |
15HY标准性能曲线图 | 25HY标准性能曲线图 |
流量公升/分钟 | ![]() | 转速RPM | 流量公升/分钟 | ![]() | 转速RPM | |
压力(公斤) | 压力(公斤) | |||||
40HY标准性能曲线图 | 65HY标准性能曲线图 | |||||
流量公升/分钟 | ![]() | 转速RPM | 流量公升/分钟 | ![]() | 转速RPM | |
压力(公斤) | 压力(公斤) |
1-(1-氯乙基)-2,3-二甲基苯
英文名:1-(1-Chloroethyl)-2,3-dimethylbenzene
CAS: 60907-88-2
质量标准:企标
包装:5Kg /20Kg(氟化桶)