绵羊白介素10(IL10)elisa试剂盒(种属齐全)技术支持
贮藏:2-8℃,避光防潮保存。有效期:6个月用途:本产品仅供科学研究规格:48T/96T(可提供免费代测服务)1.准确性:标准品线性回归与预期浓度相关系数R值,大于等于0.9900。2.灵敏度:最低检测浓度小于10 pg/ml。3.特异性:不与其它可溶性结构类似物交叉反应。4.重复性:板内、板间变异系数均小于15%。问题回答
绵羊白介素10(IL10)ELISA试剂盒(种属齐全)组成:
1、酶联板(assay plate ):一块(96孔或者48孔)。2、标准品(standard):2瓶(冻干品)。3、样品稀释液(sample diluent):1×20ml/瓶。4、生物素标记抗体稀释液(biotin-antibody diluent):1×10ml/瓶。5、辣根过氧化物酶标记亲和素稀释液 (hrp-avidin diluent):1×10ml/瓶。6、生物素标记抗体(biotin-antibody):1×120μl/瓶(1:100)7、辣根过氧化物酶标记亲和素(hrp-avidin):1×120μl/瓶(1:100)8、底物溶液(tmb substrate):1×10ml/瓶。9、浓洗涤液(wash buffer):1×20ml/瓶,使用时每瓶用蒸馏水稀释25倍。10、终止液(stop solution):1×10ml/瓶(2n h2so4)。
绵羊白介素10(IL10)ELISA试剂盒(种属齐全)
问:新鲜采集后直接零下20度冻存的可以呗,放在95%的酒精里保存的样品可以吗?答:可以的问:我们算出来的ACH含量为什么出现负值?答:说明个别样本的浓度很低,当浓度接近第6个点的时候 直线方程就有可能计算出负值问:一个孔需要多少量的血清?答:一个孔上样量微升。问:那标准品出现跳孔是怎么回事?答:你完全可以拿同样的标准品再重新做一次 ,做之前摇匀一下,肯定可以做好 。那是操作问题了 ,你可以再做一次的,之前也有客户出现过类似的问题 学生做了2次都没做好 后来老师自己做 就是摇匀了一下,其他操作都一样 标准曲线做的很完美。
绵羊白介素10(IL10)ELISA试剂盒(种属齐全)实验操作时需注意事项:
1.标准品的稀释与加样2.加样:分别设空白孔(空白对照孔不加样品及酶标试剂,其余各步操作相同)、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl(样品最终稀释度为5倍)。加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀。3.温育:用封板膜封板后置37℃温育30分钟。4.配液:将30(48t的20倍)倍浓缩洗涤液用蒸馏水30(48t的20倍)倍稀释后备用。5.洗涤:小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干。6.加酶:每孔加入酶标试剂50μl,空白孔除外。7.温育:操作同3。8.洗涤:操作同5。9.显色:每孔先加入显色剂a50μl,再加入显色剂b50μl,轻轻震荡混匀,37℃避光显色15分钟.10.终止:每孔加终止液50μl,终止反应(此时蓝色立转黄色)。11.测定:以空白空调零,450nm波长依序测量各孔的吸光度(od值)。测定应在加终止液后15分钟以内进行。
绵羊白介素10(IL10)ELISA试剂盒(种属齐全)其他产品:
兔子巨噬细胞炎性蛋白5(MIP-5)ELISA试剂盒rabbit macrophage inflammatory protein 5,MIP-5 ELISA Kit ELISA. 96T/48T小鼠血红素氧合酶2(HO-2)ELISA试剂盒Mouse heme oxygenase 2,HO-2 ELISA KitELISA. 96T/48T人肝素结合性表皮生长因子(HB-EGF)ELISA试剂盒 Human heparin-binding epidermal growth factor-like growth factor,HB-EGF ELISA KitELISA. 96T/48T人蛋白二硫化物异构酶前体(PDI)ELISA试剂盒Human Protein disulfide-isomerase precursor,PDI ELISA KitELISA. 96T/48T小鼠解脲脲原体抗体(UU-Ab)ELISA试剂盒 Mouse Ureaplasma urealyticum antibody,UU-Ab ELISA Kit ELISA. 96T/48T人抗载脂蛋白抗体A1(Apo A1)ELISA试剂盒 Human anti-apolipoprotein A1 antibody,Apo A1 ELISA KitELISA. 96T/48T大鼠白介素16(IL-16)ELISA试剂盒Rat Interleukin 16,IL-16 ELISA Kit ELISA. 96T/48T大鼠8异前列腺素(8-iso-PG)ELISA试剂盒Rat 8-iso prostaglandin,8-iso-PG ELISA KitELISA. 96T/48T人腺病毒抗原(ADV-Ag)ELISA试剂盒Human Adenovirus Antigen,ADV-Ag ELISA KitELISA. 96T/48T人抗中性粒细胞核周抗体(pANCA)ELISA试剂盒Human Perinuclear anti-neutrophil cytoplasmic antibody,pANCA ELISA KitELISA. 96T/48T人血栓调节蛋白(TM)ELISA试剂盒Human thrombomodulin,TM ELISA Kit ELISA. 96T/48T小鼠低氧诱导因子1α(HIF-1α)ELISA试剂盒Mouse hypoxia-inducible factor 1α,HIF-1α ELISA KitELISA. 96T/48T人纤调蛋白(FMOD)ELISA试剂盒Human fibromodulin,FMOD ELISA KitELISA. 96T/48T兔子白介素6(IL-6)ELISA试剂盒rabbit Interleukin 6,IL-6 ELISA KitELISA. 96T/48T人葡萄糖激酶(GCK)ELISA试剂盒Human glucokinase,GCK ELISA KitELISA. 96T/48T猪补体蛋白3(C3)ELISA试剂盒Porcine Complement 3,C3 ELISA KitELISA. 96T/48T
中文名称 | 人白介素10(IL-10)elisa测定试剂盒 | ||
英文简称 | IL-10 ELISA Kit | ||
ELISA试剂盒价格 | 面议 | 种属 | 人 |
产品规格 | 96T/Kit (8*12 strips) 48T/Kit (8*6 strips) | ||
检测方法 | 双抗夹心法、间接法 | ||
样本类型 | 体液、血清、血浆、细胞培养上清液、尿液、组织匀浆、心房水标本等等 | ||
使用范围 | 仅限科研使用,不能应用于临床 | ||
保存与运输 | 保存与运输 |
注:48T可以检测37个样本,10个标准品孔,1个空白。
96T标准的可以检测85个样本,10个标准品孔,1个空白。
科研ELISA试剂盒实验在实际操作中,除了选择优良试剂外,必须严格按照人白介素10(IL-10)elisa测定试剂盒说明书操作步骤进行操作,同时作好室内质控、室间质评,以严谨的工作作风检测每一份标本,才能保证检测质量。现在国内已有相当数量的单位拥有全自动酶标仪,这对于实现ELISA标准化检测、提高检测质量起到了积极重要的作用。
中文名称 | 英文名称 | 规格 | 保存 |
ELISA酶标板(可拆卸) | Micro ELISA Plate(Dismountable) | 96T/48T | 4℃/-20℃ # |
冻干标准品 | Reference Standard | 2/1 支 | 4℃/-20℃ # |
标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20mL/12mL | 4℃ |
浓缩生物素化抗体 | Concentrated Biotinylated Detection Ab | 1支 120μL/70μL | 4℃ |
生物素化抗体稀释液 | Biotinytated Detection Ab Diluent | 1瓶 10mL/6mL | 4℃ |
浓缩HRP酶结合物 | Concentrated HRP Conjugate | 1支 120μL/70μL | 4℃(避光) |
酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 10mL/6mL | 4℃ |
浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | Concentrated Wash Buffer (25×) | 4℃ |
底物溶液(TMB) | Substrate Reagent | 1瓶 10mL/6mL | 4℃(避光) |
反应终止液 | Stop Solution | 1瓶 10mL/6mL | 4℃ |
封板覆膜 | Plate Sealer | 2 张 |
|
产品说明书 | Product Description | 1 份 |
|
特别说明: *: [96T/48T](打开包装后请及时检查所有物品是否齐全完整) #: 一周内使用可存于4℃,需长时间存放或多次使用建议存于-20℃. |
人白介素10(IL-10)elisa测定试剂盒操作步骤:
1.包被:用0.05MPH9.牰碳酸盐包被缓冲液将抗体稀释至蛋白质含量为1~10μg/ml。在每个聚苯乙烯板的反应孔中加0.1ml,4℃ 过一夜。次日,弃去孔内溶液,用洗涤缓冲液洗3次,每次3分钟。(简称洗涤,下同)。
2.加样:加一定稀释的待检样品0.1ml于上述已包被之反应孔中,置37℃ 孵育1小时。然后洗涤。(同时做空白孔,阴性对照孔及阳性对照孔)。
3.加酶标抗体:于各反应孔中,加入新鲜稀释的酶标抗体(经滴定后的稀释度)0.1ml。37℃ 孵育0.5~1小时,洗涤。
4.加底物液显色:于各反应孔中加入临时配制的TMB底物溶液0.1ml,37℃ 10~30分钟。
5.终止反应:于各反应孔中加入2M硫酸0.05ml。
6.结果判定:可于白色背景上,直接用肉眼观察结果:反应孔内颜色越深,阳性程度越强,阴性反应为无色或极浅,依据所呈颜色的深浅,以“+”、“-”号表示。也可测OD值:在ELISA检测仪上,于450nm(若以ABTS显色,则410nm)处,以空白对照孔调零后测各孔OD值,若大于规定的阴性对照OD值的2.1倍,即为阳性。
人白介素10(IL-10)elisa测定试剂盒上海润裕生物科技有限公司针对不同行业的需求,我司不断完善、更新技术和方法。在实验室应用的比较广泛,被业内众多的科研人士认可和推广。公司以优质,齐全的产品和优质的技术团队得到了国家各大实验高校及科研单位的认可和赞誉,拥有很好的知名度和口碑。如需要更多科研ELISA试剂盒说明书及报价等相关信息,请来电咨询。免费代测人白介素10(IL-10)elisa试剂盒
Y10058A | 人白介素1可溶性受体Ⅱ(IL-1sRⅡ)Elisa试剂盒 |
Y10059A | 人白介素1可溶性受体Ⅰ(IL-1sRⅠ)Elisa试剂盒 |
Y10060A | 人白介素16(IL-16)Elisa试剂盒 |
Y10061A | 人白介素15(IL-15)Elisa试剂盒 |
Y10062A | 人白介素13(IL-13)Elisa试剂盒 |
Y10063A | 人白介素12(IL-12/P70)Elisa试剂盒 |
Y10064A | 人白介素12(IL-12/P40)Elisa试剂盒 |
Y10065A | 人白介素11(IL-11)Elisa试剂盒 |
Y10066A | 人白介素10(IL-10)Elisa试剂盒 |
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Y10058A | 人白介素1可溶性受体Ⅱ(IL-1sRⅡ)Elisa试剂盒 |
Y10059A | 人白介素1可溶性受体Ⅰ(IL-1sRⅠ)Elisa试剂盒 |
Y10060A | 人白介素16(IL-16)Elisa试剂盒 |
Y10061A | 人白介素15(IL-15)Elisa试剂盒 |
Y10062A | 人白介素13(IL-13)Elisa试剂盒 |
Y10063A | 人白介素12(IL-12/P70)Elisa试剂盒 |
Y10064A | 人白介素12(IL-12/P40)Elisa试剂盒 |
Y10065A | 人白介素11(IL-11)Elisa试剂盒 |
Y10066A | 人白介素10(IL-10)Elisa试剂盒 |
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上海卒瑞生物科技有限公司是国内专业研发生产Elisa试剂盒、金标检测试剂盒、酶联法检测试剂盒的供应商,且供销各类生化试剂(进口国产)、培养基、动物血清、抗原、抗体、实验试剂、人及动物Elisa试剂盒、氨基酸类、实验室耗材、实验室仪器、低温恒温槽、雪花制冰机、恒温油槽等各类产品。本公司是专门从事生物科技领域内的技术开发、技术咨询、销售为一体的科学技术服务公司。为国内外科研院所、学校、实验室、制药、化工、环境保护及卫生检疫部门等相应企业提供科研实验技术服务。我们拥有先进的研发技术和雄厚的科学实力,完整、科学的质量管理体系。本公司以诚信、实力和产品质量获得业界的认可,与多家生产商建立了长期稳定的合作关系。欢迎各界朋友莅临参观、指导和业务洽谈。
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人白介素10(IL-10)Elisa快速检测试剂盒
特 异 性:本elisa试剂盒可同时检测天然或重组的,且与其他相关蛋白无交叉反应。
检测种属:人、大小鼠、兔、羊、猴、猪、豚鼠ELISA检测试剂盒等种属。
样本范围:血清、血浆、细胞上清液、尿液、体液、灌洗液、脑脊髓、心房水、胸房水、组织等。
储存方式人白介素10(IL-10)Elisa快速检测试剂盒
存储条件:4℃保存
有 效 期:6个月
产品规格:96T/48T
适用范围:此试剂盒仅用于科研实验,严禁用于临床。
产品特点人白介素10(IL-10)Elisa快速检测试剂盒本公司对所有售出的产品保证质量,并提供良好的售后服务,凡购买本公司任意款Elisa试剂盒,均可享受免费代测服务,并可全程提供技术指导。
用特异性抗体包被于固相载体,经洗涤后加入含有抗原之待测样品,如待检样品中有相应抗原存在,即可与包被于固相载体上的特异性抗体结合,经保温孵育洗涤后,即可加入酶标记特异性抗体,再经孵育洗涤后,加底物显色进行测定,底物降解的量即为欲测抗原的量。这种方法欲测的抗原必须有两个可以与抗体结合的部位,因为其一端要包被于固相载体上的抗体作用,而另一端则要与酶标记特异性抗体作用。因此,不能用于分子量小于5000的半抗原之类的抗原测定。
可以:(1)免疫酶染色各种细胞内成份的定位。
(2)研究抗酶抗体的合成。
(3)显现微量的免疫沉淀反应。
(4)定量检测体液中抗原或抗体成份。
血浆样本直接测定前处理方法:
(1)加酶抑制剂:准确采全血若干毫升,注入预冷的加有抗凝剂的塑料指形管中,迅速低温离心,取血浆低温保存待测。抑肽酶有液体的与固体的。固体的抑肽酶可用生理盐水溶解。
(2)酸化处理:每毫升血浆加1mol/l HCl 0.2ml,低温保存待测。以上两种方法,任选一种即可。
血浆样本的间接测定 血标本经提取后,可去除蛋白质等干扰因素,并使微量的生物活性肽浓缩,但较费时,成本也高。常用的提取方法有:
(1)柱层析提取法:有多种层析柱可供提取不同的神经肽,其中较为方便的是用Sep-pak C18层析柱提取。主要步骤:
①柱的预处理:该柱有两头,长头连接注射器,可注入溶液与样品,短头为排出口。预处理时注入乙腈10ml。蒸馏水20ml,使柱中的生物胶活化。
②注入样品(如血浆、脑脊液、腹水、尿等):样品中的水份流出,生物活性肽、蛋白及杂质等吸附在生物胶上。血浆上柱前,如先去除蛋白,可增加柱子的使用时间。
③淋洗:用4%HAC溶液100ml注入柱中,以洗去一些杂质。
④洗脱:用7ml酸性乙腈作为洗脱剂,注入柱中,把生物活性肽洗脱下来,收集在塑料指形管中。
⑤清洗:用乙腈、蒸馏水清洗柱子,以备后用。
⑥将洗脱液吹干,低温保存待测。测定时可加入一定量的缓冲液溶解。
人白介素10(IL-10)Elisa快速检测试剂盒
YS01050B人细胞凋亡抑制因子(IAP)elisa试剂盒
YS01051B人集落刺激因子(CSF)Elisa试剂盒
YS01052B人γ干扰素诱导单核细胞因子(MIGF)Elisa试剂盒
YS01053B人干扰素诱导T细胞趋化因子(ITAC)Elisa试剂盒
YS01054B人CD14分子(CDl4)Elisa试剂盒
YS01055B人凋亡诱导因子(AIF)Elisa试剂盒
YS01056B人白细胞共同抗原(LCA)Elisa试剂盒
YS01057B人CD4分子(CD4)Elisa试剂盒
YS01058B人P钙黏蛋白/胎盘钙黏蛋白(P-cad)Elisa试剂盒
YS01059B人角化细胞生长因子(KGF)Elisa试剂盒
YS01060B人血小板衍生生长因子BB(PDGF-BB)Elisa试剂盒
YS01061B人CXC趋化因子配体16(CXCL16)Elisa试剂盒
YS01062B人CXC趋化因子受体3(CXCR3)Elisa试剂盒
YS01063B人γ干扰素诱导蛋白16(IFI16)Elisa试剂盒
YS01064B人基质细胞衍生因子1a(SDF-1a)Elisa试剂盒
YS01065B人淋巴细胞趋化因子(Lptn)Elisa试剂盒
YS01066B人α干扰素(IFN-α)Elisa试剂盒
YS01067B人可溶性CD86(sCD86)Elisa试剂盒
YS01068B人白介素27(IL-27)Elisa试剂盒
YS01069B人白介素23(IL-23)Elisa试剂盒
YS01070B人巨噬细胞移动抑制因子(MIF)Elisa试剂盒
YS01071B人组织因子途径抑制物(TFPI)Elisa试剂盒
YS01072B人干扰素诱导蛋白10(IP-10)Elisa试剂盒
YS01073B人白介素1(IL-1)Elisa试剂盒
YS01074B人白介素17(IL-17)Elisa试剂盒
YS01075B人白介素1β(IL-1β)Elisa试剂盒
YS01076B人表皮生长因子(EGF)Elisa试剂盒
YS01077B人碱性成纤维细胞生长因子(bFGF)Elisa试剂盒
YS01078B人巨噬细胞炎性蛋白5(MIP-5)Elisa试剂盒
YS01079B人可溶性E选择素(sE-selectin)Elisa试剂盒
YS01080B人可溶性细胞间粘附分子1(sICAM-1)Elisa试剂盒
YS01081B人细胞间粘附分子2(ICAM-2)Elisa试剂盒
YS01082B人细胞间粘附分子3(ICAM-3)Elisa试剂盒
YS01083B人结缔组织生长因子(CTGF)Elisa试剂盒
YS01084B人白介素18(IL-18)Elisa试剂盒
YS01085B人粘膜相关上皮趋化因子(MEC)Elisa试剂盒
YS01086B人B细胞活化因子受体(BAFF-R)Elisa试剂盒
YS01087B人血管内皮细胞生长因子受体3(VEGFR-3)Elisa试剂盒
YS01088B人血管内皮细胞生长因子受体1(VEGFR-1)Elisa试剂盒
YS01089B人血管内皮细胞生长因子D(VEGF-D)Elisa试剂盒
YS01090B人血管内皮细胞生长因子C(VEGF-C)Elisa试剂盒
YS01091B人血管内皮细胞生长因子A(VEGF-A)Elisa试剂盒
YS01092B人血管内皮细胞生长因子B(VEGF-B)Elisa试剂盒
YS01093B人血管内皮细胞生长因子(VEGF)Elisa试剂盒
YS01094B人血管内皮细胞粘附分子1(VCAM-1)Elisa试剂盒
YS01095B人可溶性肿瘤坏死因子相关凋亡诱导配体(sTRAIL)Elisa试剂盒
YS01096B人肿瘤坏死因子相关凋亡诱导配体4(TRAIL-R4)Elisa试剂盒
YS01097B人肿瘤坏死因子相关凋亡诱导配体3(TRAIL-R3)Elisa试剂盒
YS01098B人肿瘤坏死因子相关凋亡诱导配体1(TRAIL-R1)Elisa试剂盒
YS01099B人肿瘤坏死因子β(TNF-β)Elisa试剂盒
YS01100B人肿瘤坏死因子α(TNF-α)Elisa试剂盒
YS01101B人肿瘤坏死因子可溶性受体Ⅱ(TNFsR-Ⅱ)Elisa试剂盒
YS01102B人肿瘤坏死因子可溶性受体Ⅰ(TNFsR-Ⅰ)Elisa试剂盒
YS01103B人转化生长因子β1(TGF-β1)Elisa试剂盒
YS01104B人转化生长因子α(TGF-α)Elisa试剂盒
YS01105B人基质细胞衍生因子1β(SDF-1β)Elisa试剂盒
YS01106B人干细胞因子受体(SCFR)Elisa试剂盒
YS01107B人干细胞因子/肥大细胞生长因子(SCF/MGF)Elisa试剂盒
YS01108B人可溶性CD40配体(sCD40L)Elisa试剂盒
YS01109B人可溶性CD30配体(sCD30L)Elisa试剂盒
操作流程我公司现货供应,全国包邮,提供免费代测服务,全程提供技术支持,质量保证,欢迎新老客户电话咨询我公司免费索取该试剂盒或相关Elisa试剂盒的详细说明书及价格信息。客户好评欢迎新老客户咨询订购:人白介素10(IL-10)Elisa快速检测试剂盒
规格:96T/48T
货期:2~3天
保存温度:2-8℃
用途:用于测定兔血清、血浆、组织及相关液体样本中的含量或活性。
有效期:12个月(-20℃)/6个月(2-8℃)。
检测波长:450nm。
所需样本体积:50-100ul。
人白介素10(IL10)elisa试剂盒检测目的:用于测定血清、血浆及相关液体等样本(如:血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本)。
供应种属:人、小鼠、大鼠、豚鼠、兔、猪、犬、牛、羊、鸡、鸭、植物等ELISA试剂盒。
标本类型:包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等,注:标本必须为液体,不含沉淀。
ELISA常用的方法:双抗体夹心法、间接法、竞争法以及BAS-ELISA等。
温馨提示:将标本及时分装后放在-20℃或-70℃条件下保存。避免反复冻融。标本2-8℃可保存48小时,-20℃可保存1个月。-70度可保存6个月。
人白介素10(IL10)elisa试剂盒等润裕生物elisa试剂盒产品优势:
1.专一性强。抗原与抗体的免疫反应是专一反应,而免疫酶技术以免疫反应为基础,所检测的对象是抗原(或抗体),使用的抗体除标记了酶以外,与普通抗体的免疫反应特性并无多大差别。
2.灵敏度高。由于抗体联结上了酶,因此,借助于酶与底物的显色反应,显示抗原与抗体的结合,大大提高了检测的灵敏性,使检测水平接近放射免疫测定法。
3.样品易保存。经过酶反应显示的有色产物大多比较稳定,因此有利于样品的保存。
4.结果易观察。对检测结果即可用肉眼观察,又可用显微镜观察,也可以用分光光度计进行比色测定,还可用显微镜观察。这是因为某些酶反应产物能使电子密度发生改变,从而引起被检测物的显示。
5.可以定量测定。溶液中的抗原物质,应用酶免吸附技术进行比色测定,依据光密度值的变化,可以定量。预计用细胞分光光度计可对组织内的抗原进行免疫酶技术的定量测定。
6.可以大规模测定样品。如有成千上万份样品需要进行检测,免疫酶技术都能在较短时间内完成。
7.仪器和试剂简单。对免疫没技术来说,不需要荧光显微镜,也不需要测定放射性的特殊仪器,所用仪器及试剂均属一般性仪器与试剂,普通实验室及生产应用单位均易购置。
人白介素10(IL10)elisa试剂盒等润裕生物ELISA试剂盒产品,原装ELISA试剂盒以及各类国产ELISA试剂盒产品8折优惠热销中,欢迎广大科研学者前来选购:RY-(elisa)-00145猪低分子肝素(LMWH)ELISA试剂盒Porcine low molecular weight heparin,LMWH ELISA Kit规格:96T/48TRY-(elisa)-00146猪巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA试剂盒Porcine macrophage inflammatory protein 1α,MIP-1α ELISA Kit规格:96T/48TRY-(elisa)-00147猪主要急性期蛋白( MAP)ELISA试剂盒Porcine MAP ELISA Kit规格:96T/48TRY-(elisa)-00148猪基质金属蛋白酶9(MMP-9)ELISA试剂盒 Porcine matrix metalloproteinase 9,MMP-9 ELISA Kit规格:96T/48TRY-(elisa)-00149猪黑色素瘤转移表面黏附分子(MMSAM)ELISA试剂盒Porcine melanoma metastasis surface adhesion molecule,MMSAM ELISA Kit规格:96T/48TRY-(elisa)-00150猪褪黑素(MT)ELISA试剂盒Porcine Melatonin,MT ELISA Kit规格:96T/48TRY-(elisa)-00151猪高铁血红蛋白(MHB)ELISA试剂盒Porcine Methemoglobin,MHB ELISA Kit规格:96T/48TRY-(elisa)-00152猪髓磷脂碱性蛋白(MBP)ELISA试剂盒Porcine myelin basic protein,MBP ELISA Kit规格:96T/48TRY-(elisa)-00153猪髓过氧化物酶(MPO)ELISA试剂盒Porcine myeloperoxidase,MPO ELISA Kit规格:96T/48TRY-(elisa)-00154猪一氧化氮(NO)ELISA试剂盒Porcine nitric oxide,NO ELISA Kit规格:96T/48TRY-(elisa)-00155猪去甲肾上腺素(NE)ELISA试剂盒Porcine Noradrenaline,NE ELISA Kit规格:96T/48TRY-(elisa)-00156猪Ⅲ型前胶原肽(PⅢNP)ELISA试剂盒Porcine N-terminal procollagen Ⅲ propeptide,PⅢNP ELISA Kit规格:96T/48TRY-(elisa)-00157猪食欲素/阿立新B(OX-B)ELISA试剂盒Porcine Orexin B,OX-B ELISA Kit规格:96T/48TRY-(elisa)-00158猪食欲素受体(OXR)ELISA试剂盒Porcine Orexin receptor,OXR ELISA Kit规格:96T/48TRY-(elisa)-00159猪骨钙素/骨谷氨酸蛋白(OT/BGP)ELISA试剂盒Porcine Osteocalcin/Bone gla protein,OT/BGP ELISA kit规格:96T/48TRY-(elisa)-00160猪氧化低密度脂蛋白(OxLDL)ELISA试剂盒Porcine oxidized lowdensity lipoprotein,OxLDL ELISA Kit规格:96T/48TRY-(elisa)-00161猪磷酸化细胞外信号调节激酶(pERK)ELISA试剂盒Porcine phospho-extracellular signal-regulated kinase,pERK ELISA Kit规格:96T/48TRY-(elisa)-00162猪纤溶酶原激活物抑制因子1(PAI-1)ELISA试剂盒Porcine plasminogen activator inhibitor 1,PAI-1 ELISA Kit规格:96T/48TRY-(elisa)-00163猪纤溶酶原激活物抑制因子(PAI)ELISA试剂盒Porcine plasminogen activator inhibitor,PAI ELISA Kit规格:96T/48TRY-(elisa)-00164猪血小板活化因子(PAF)ELISA试剂盒Porcine platelet activating factor,PAF ELISA Kit规格:96T/48TRY-(elisa)-00165猪血小板衍生生长因子(PDGF)ELISA试剂盒Porcine platelet-derived growth factor,PDGF ELISA Kit规格:96T/48TRY-(elisa)-00166猪Ⅲ型前胶原氨基端肽(PⅢNT)ELISA试剂盒Porcine procollagen Ⅲ N-terminal peptide,PⅢNT ELISA Kit规格:96T/48TRY-(elisa)-00167猪孕激素/孕酮(PROG)ELISA试剂盒Porcine Progesterone,PROG ELISA Kit规格:96T/48TRY-(elisa)-00168猪肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒Porcine Pulmonary surfatcant-associated protein A,SP-A ELISA Kit规格:96T/48TRY-(elisa)-00169猪松弛肽/松弛素(RLN)ELISA试剂盒Porcine Relaxin,RLN ELISA Kit规格:96T/48TRY-(elisa)-00170猪钠-葡萄糖共转运载体1(SGLT1)ELISA试剂盒Porcine sodium-glucose cotransporter 1,SGLT1 ELISA Kit规格:96T/48TRY-(elisa)-00171猪可溶性E选择素(sE-selectin)ELISA试剂盒 Porcine soluble E-selectin,sE-selectin ELISA Kit规格:96T/48TRY-(elisa)-00172猪可溶性细胞间粘附分子1(sICAM-1)ELISA试剂盒Porcine Soluble Intercellular Adhesion Molecule-1,sICAM-1 ELISA Kit规格:96T/48TRY-(elisa)-00173猪可溶性P选择素(sP-Selectin)ELISA试剂盒Porcine soluble P-selectin,sP-Selectin ELISA Kit规格:96T/48TRY-(elisa)-00174猪可溶性血管内皮生长因子受体2(VEGFR-2/sFLK-1)ELISA试剂盒Porcine soluble vascular endothelial growth factor receptor-2,VEGFR-2/sFLK-1 ELISA Kit规格:96T/48T
上海美旋生物科技有限公司竭诚为您提供优质的elisa试剂盒产品服务,价格实惠,量大更优惠,敬请您来电咨询!
中文名称:人白介素10(IL-10)ELISA试剂盒
说明书:欢迎来电索取,通常会随ELISA试剂盒一起寄出。
规格:48T/96T(仅用于科研,不得用于临床诊断)
特异性:本试剂盒可同时检测天然或重组的,且与其他相关蛋白无交叉反应。
特点:灵敏性高、特异性强、重复性好
人白介素10(IL-10)ELISA试剂盒现货供应,量大更优惠,我公司是专业的高品质ELISA试剂盒供应商,经过长年发展已成为全国各大型科研院所ELISA试剂盒指定供应商与战略合作伙伴,敬请您来电咨询!
如需人白介素10(IL-10)ELISA试剂盒说明书请点击网页“我要询价”,填写询价留言表(↓↓↓),或者通过网页上我司的联系方式与我们联系,电话、在线客服QQ、发送电子邮件均可,美旋生物专业供应各种类高品质Elisa试剂盒,让您买得舒心,用得满意,为您的科研工作提供优质保障!购买前请联系我公司销售人员了解产品的详细信息及库存情况,美旋生物祝您工作顺利,生活愉快!
人白介素10(IL-10)ELISA试剂盒现货供应,量大更优惠,全国免费送货上门:
MX-12761 小鼠硫氧化还原蛋白(Trx)ELISA试剂盒 英文名:Mouse Thioredoxin,Trx ELISA Kit 规格:48T/96TMX-12762 小鼠胶原酶II(Collagenase II)ELISA试剂盒 英文名:Mouse Collagenase Type II ELISA Kit 规格:48T/96TMX-12763 小鼠胶原酶I(Collagenase I)ELISA试剂盒 英文名:Mouse Collagenase I ELISA Kit 规格:48T/96TMX-12764 小鼠磷酸化蛋白激酶C(P-PKC)ELISA试剂盒 英文名:Mouse phospho Protein Kinase C,P-PKC ELISA Kit 规格:48T/96TMX-12765 小鼠磷酸化细胞外信号调节激酶(pERK)ELISA试剂盒 英文名:Mouse phospho-extracellular signal-regulated kinase,pERK ELISA Kit 规格:48T/96TMX-12766 小鼠钙通道阻滞剂(CCB)ELISA试剂盒 英文名:Mouse calcium channel blockers,CCB ELISA Kit 规格:48T/96TMX-12767 小鼠丙氨酸转氨酶/谷丙转氨酶(ALT/GPT)ELISA试剂盒 英文名:Mouse Alanine Aminotransferase,ALT/GPT ELISA Kit 规格:48T/96TMX-12768 小鼠骨形成蛋白(BMPs)ELISA试剂盒 英文名:Mouse bone morphogenetic proteins,BMPs ELISA Kit 规格:48T/96TMX-12769 小鼠视黄醇结合蛋白4(RBP-4)ELISA试剂盒 英文名:Mouse Retinol-binding protein 4,RBP-4 ELISA Kit 规格:48T/96TMX-12770 小鼠6酮前列腺素F1a(6-keto-PGF1a)ELISA试剂盒 英文名:Mouse 6-keto-prostaglandin F1a,6-K-PGF1a ELISA Kit 规格:48T/96TMX-12771 小鼠戊糖素(Pentosidine)ELISA试剂盒 英文名:Mouse Pentosidine ELISA Kit 规格:48T/96TMX-12772 小鼠晚期糖基化终末产物(AGEs)ELISA试剂盒 英文名:Mouse advanced glycation end products,AGEs ELISA Kit 规格:48T/96TMX-12773 小鼠中性粒细胞明胶酶相关脂质运载蛋白(NGAL)ELISA试剂盒 英文名:Mouse neutrophil gelatinase-associated lipocalin,NGAL ELISA Kit 规格:48T/96T
本公司长期供应人白介素10(IL-10)ELISA试剂盒,全方位售前、售中、售后服务,为您解决实验过程中的问题。
小鼠白介素10(IL-10)酶免测定elisa试剂盒价格,北京现货代检测试剂盒种类 种属 规格
细胞因子检测试剂盒 人/小鼠/大鼠/其他 48T/96T
内分泌检测试剂盒 人/小鼠/大鼠/其他 48T/96T
肝纤维化检测试剂盒 人/小鼠/大鼠/其他 48T/96T
心肌梗塞检测试剂盒 人/小鼠/大鼠/其他 48T/96T
肿瘤标志物检测试剂盒 人/小鼠/大鼠/其他 48T/96T
自身免疫检测试剂盒 人/小鼠/大鼠/其他 48T/96T
优生优育检测试剂盒 人/小鼠/大鼠/其他 48T/96T
传染病检测试剂盒 人/小鼠/大鼠/其他 48T/96T
ELISA可检测标本:血清、血浆、细胞上清液、尿液、灌洗液、脑脊髓、心房水、胸腹水、组织等。
Mouse interleukin 10,IL-10 ELISA kit 加拿大HCB公司ELISA试剂盒价格 北京代理,elisa实验样本收集:(1)血清(血浆)样本:取全血加入促凝管(抗凝管),2500转离心20分钟左右,收集上清,液氮或者-80℃冰箱保存;(2)尿液样本:样本2500转离心20分钟左右,液氮或者-80℃冰箱保存;胸腹水、脑脊液、肺泡灌洗液参照此实行;(3)细胞样本:检测分泌性的成份时,样本2500转离心20分钟左右,液氮或者-80℃冰箱保存;检测细胞内的成份时,用PBS稀释细胞悬液,通过反复冻融,以使细胞破坏并放出细胞内成份,2500转离心20分钟左右,收集上清同上;(4)组织样本:切割标本后,称取重量,用液氮或者-80℃冰箱冷冻保存备用。(5)标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,有条件的,最好-70℃冻存备用,但应避免反复冻融.(6)关于样本收集更详细信息,请联系我公司技术人员。
小鼠白介素10(IL-10)酶免测定ELISA试剂盒价格,北京现货代检测 北京代理-酶联免疫试剂盒供货商 elisa试剂盒供应厂家 可以免费代测 保证质量 无效退款!
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Rat Interleukin 10(IL-10)
ELISA Kit
Catalog No. CSB-E04595r
(96T)
l This immunoassay kit allows for the in vitro quantitative determination of rat IL-10 concentrations in serum, plasma and Tissue Homogenates.
l Expiration date six months from the date of manufacture
l FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
PRINCIPLE OF THE ASSAY
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
DETECTION RANGE
3.12 pg/ml-200 pg/ml. The standard curve concentrations used for the ELISA’s were 200 pg/ml, 100 pg/ml, 50 pg/ml, 25 pg/ml, 12.5 pg/ml, 6.25 pg/ml, 3.12 pg/ml.
SPECIFICITY
This assay recognizes rat IL-10. No significant cross-reactivity or interference was observed.
SENSITIVITY
The minimum detectable dose of rat IL-10 is typically less than 0.78 pg/ml.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.
MATERIALS PROVIDED
Reagent | Quantity |
Assay plate | 1 |
Standard | 2 |
Sample Diluent | 1 x 20 ml |
Biotin-antibody Diluent | 1 x 10 ml |
HRP-avidin Diluent | 1 x 10 ml |
Biotin-antibody | 1 x 120μl |
HRP-avidin | 1 x 120μl |
Wash Buffer | 1 x 20 ml (25×concentrate) |
TMB Substrate | 1 x 10 ml |
Stop Solution | 1 x 10 ml |
STORAGE
1. Unopened test kits should be stored at 2-8°C upon receipt and the microtiter plate should be kept in a sealed bag. The test kit may be used throughout the expiration date of the kit, provided it is stored as prescribed above. Refer to the package label for the expiration date.
2. Opened test plate should be stored at 2-8°C in the aluminum foil bag with desiccants to minimize exposure to damp air. The kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
3. A microtiter plate reader with a bandwidth of 10 nm or less and an optical density range of 0-3 OD or greater at 450nm wavelength is acceptable for use in absorbance measurement.
REAGENT PREPARATION
Bring all reagents to room temperature before use.
1. Wash Buffer If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to prepare 500 ml of Wash Buffer.
2. Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 ml of Sample Diluent. This reconstitution produces a stock solution of 200 pg/ml. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making serial dilutions. The undiluted standard serves as the high standard (200 pg/ml). The Sample Diluent serves as the zero standard (0 pg/ml). Prepare fresh for each assay. Use within 4 hours and discard after use.
3. Biotin-antibody Centrifuge the vial before opening. Dilute to the working concentration using Biotin-antibody Diluent(1:100), respectively.
4. HRP-avidin Centrifuge the vial before opening. Dilute to the working concentration using HRP-avidin Diluent(1:100), respectively.
Precaution: The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
OTHER SUPPLIES REQUIRED
Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
Pipettes and pipette tips.
Deionized or distilled water.
Squirt bottle, manifold dispenser, or automated microplate washer.
An incubator which can provide stable incubation conditions up to 37°C±0.5°C.
SAMPLE COLLECTION AND STORAGE
l Serum Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at 1000 g. Remove serum and assay immediately or aliquot and store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
l Plasma Collect plasma using citrate, EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 g within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
l Tissue Homogenates 100mg tissue was rinsed with 1X PBS, homogenized in 1 mL of 1X PBS and stored overnight at -20° C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. The supernate was assayed and removed immediately. Alternatively, aliquot and store samples at -20°C or -80℃. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
Note: Grossly hemolyzed samples are not suitable for use in this assay.
ASSAY PROCEDURE
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate. All the reagents should be added directly to the liquid level in the well. The pipette should avoid contacting the inner wall of the well.
1. Add 100μl of Standard, Blank, or Sample per well. Cover with the adhesive strip. Incubate for 2 hours at 37°C.
2. Remove the liquid of each well, don’t wash.
3. Add 100μl of Biotin-antibody working solution to each well. Incubate for 1 hour at 37°C. Biotin-antibody working solution may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.
4. Aspirate each well and wash, repeating the process three times for a total of three washes. Wash: Fill each well with Wash Buffer (200μl) and let it stand for 2 minutes, then remove the liquid by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel. Complete removal of liquid at each step is essential to good performance.
5. Add 100μl of HRP-avidin working solution to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
6. Repeat the aspiration and wash five times as step 4.
7. Add 90μl of TMB Substrate to each well. Incubate for 10-30 minutes at 37°C. Keeping the plate away from drafts and other temperature fluctuations in the dark.
8. Add 50μl of Stop Solution to each well when the first four wells containing the highest concentration of standards develop obvious blue color. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
9. Determine the optical density of each well within 30 minutes, using a microplate reader set to 450 nm.
CALCULATION OF RESULTS
Using the professional soft "Curve Exert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard, control, and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL-10 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
LIMITATIONS OF THE PROCEDURE
The kit should not be used beyond the expiration date on the kit label.
Do not mix or substitute reagents with those from other lots or sources.
It is important that the Standard Diluent selected for the standard curve be consistent with the samples being assayed.
If samples generate values higher than the highest standard, dilute the samples with the appropriate Standard Diluent and repeat the assay.
Any variation in Standard Diluent, operator, pipetting technique, washing technique, incubation time or temperature, and kit age can cause variation in binding.
This assay is designed to eliminate interference by soluble receptors, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
TECHNICAL HINTS
Centrifuge vials before opening to collect contents.
When mixing or reconstituting protein solutions, always avoid foaming.
To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
When using an automated plate washer, adding a 30 second soak period following the addition of wash buffer, and/or rotating the plate 180 degrees between wash steps may improve assay precision.
To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary.
Substrate Solution should remain colorless or light blue until added to the plate. Keep Substrate Solution protected from light. Substrate Solution should change from colorless or light blue to gradations of blue.
Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution. Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.