联系我们

联系人：梁经理

点击查看联系方式

供应分类

产品展示

Human Dopamine（DA） ELISA Kit报价

点击次数：204

价格：￥电议

更新日期：2026/8/25 0:23:37

生产地：中国大陆

产品型号：

简单介绍：Human Dopamine（DA） ELISA Kit报价
英文名称：产品类别：Humam ELISA Kit
商品品牌：SANCHEZ C AS 号：eia05559h
分子式：分子量：
包装类型：盒装产品库存：100
[批发价格]
规格价格纯度
96T电议
1
立即订购

商家名片

梁**

联系我时，请说明是阿仪网看到的，谢谢!

电话: 40**************************同步）
手机: 13******491
微信:
交换名片后展示联系方式 → →

相关标签：Human,Dopamine（DA）,ELISA,Kit

详细内容

Cat No.eia05559h

Human Dopamine(DA) ELISA Kit

Dear customers, thank you for choosing our products. This product is suitable for in vitro qualitative detection of human serum, plasma or cell culture supernatant ａnd organizations in the natural ａnd recombinant DA concentration. Detection of other special sample please contact our technical support. The kit is for research use only. Please read the instructions carefully before using ａnd check the kit components. If you have any questions, please contact SANCHEZ INC You will get our full range of services.

This kit employs Double Antibody Sandwich Technique. The principle of Double Antibody Sandwich is based on characteristics of the tested antigen with more than two valances which can identify coated antibody and detection antibody at same time. The specific process is as follows:

1. Connect the specific antibodies ａnd solid phase carriers to form immobilized antibodies. Wash out uncombined antibodies ａnd impurities. Seal the rest binding sites with irrelevant proteins.

2. Join under test with immobilized antibodies for contact reaction. After a while, combine antigens in ａnd antibodies on carriers into the antigens complex. Wash out uncombined antibodies ａnd impurities.

3. Add biotin labeling antibodies to combine with the antigens on immune complexes. Wash out the uncombined biotin labeling antibodies thoroughly. The enzyme amount on the carrier is now positively related to the amount of the tested substance in specimens.

4. Add horseradish peroxidase to label the avidins ａnd incorporate them with the biotin labeling antibodies. Wash out the incorporated enzyme markers thoroughly. The enzyme amount on the carrier is now positively related to the amount of the tested substance in specimens.

5. Add substrates for coloring, ａnd compute the concentration of specimens.

Note: an antibody molecule can be marked on several biotin molecules ａnd a biotin molecule can be connected with a HRP-Avidin to form numbers of horseradish peroxidases combining with antibodies which shows higher sensitivity ａnd amplification effect comparing with traditional direct HRP-Antibodies.

【Detection principle of Human Dopamine(DA) ELISA kit】

This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human DA monoclonal antibody ａnd the detecting antibody is polyclonal antibody with biotin labeled. Samples ａnd biotin labeling antibody are added into ELISA plate wells ａnd washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic ａnd finally turns into yellow under the action of acid. The color depth ａnd the testing factors in samples are positively correlated.

【Kit composition】

name 96 Tests 48 TestsStorage

1．antibody precoated plate 8×12 8×64/-20℃

2．Human DA Standards 2 vial 1 vial4/-20℃

3．Biotinylated antibody(1:100) 1 vial 1 vial4/-20℃

4．Enzyme conjugate(1:100) 1vial 1 vial4/-20℃

5．Enzyme diluent 1vial 1vial 4/-20℃

6．antibody diluent 1vial 1 vial4/-20℃

7．Standard diluent 1vial 1 vial 4/-20℃

8．Sample diluent 1vial 1 vial 4/-20℃

9．Washing buffer (1:25) 1 vial 1 vial 4/-20℃

10．Colour Reagent A 1 vial 1 vial 4/-20℃

11．Colour Reagent B1 vial 1 vial 4/-20℃

12．Colour Reagent C 1 vial 1 vial 4/-20℃

13．Instruction 1 set 1 set RT

Note：

RT：Room temperature

Standard：Frozen dried

Colour Reagent A: Avoid light

【Necessary for testing their own test facilities ａnd equipment】

1. Microplate reader (450nm detection wavelength filter, 570nm or 630nm correction wavelength filters).

2. Washer (adjustable amount of liquid injection to ensure that each well 350μl lotion without overflow).

3. Clean benches, biological safety cabinets, fume hoods.

4. High-precision single-channel dispenser (range 0.5-10μl-20μl, 20-200μl, 200-1000μl).

5. High-precision multi-channel plus liquid (8 or 12, the range of 50-300μl of).

6. 37℃ incubator.

7. Low temperature centrifuge.

8. Refrigerators (4℃, -20℃, -86℃).

9. Analytical balance.

10. Scissors, tweezers, pliers, ａnd so on.

11. Swirl mixing device, low-frequency oscillator, ａnd so on.

【Necessary for testing their own testing supplies ａnd reagents】

1. Centrifuge tube (capacity of 1.5ml, 5ml, ａnd so on).

2. Disposable tip (range of 0.5-10μl-20μl, 20-200μl, 200-1000μl).

3. Pure water or distilled water.

4. Coordinate paper.

5. Absorbent paper.

6. EDTA, sodium citrate, heparin

【Sample collection Note】

1. The tube for blood collection should be free of pyrogen and endotoxin

2. Hemolysis and hyperlipidemia specimens can not be used to extracted serum ａnd plasma.

3. The samples should appear clear ａnd transparent. And all the suspension should be removed through centrifugation.

4. If collected samples are not timely detected, they should be divided according to single usage amount ａnd frozen reserved in refrigerator at -20-80℃, avoiding the repeated freeze-thaw.

Human,Dopamine（DA）,ELISA,Kit

采购记录

优质供应

我要推广

优质信息推广广告

友情链接
热词推荐

已获得阿仪网信誉等级评定

产品展示