兔抗大鼠/小鼠脂筏蛋白-1抗体
体积：100ug
介绍：筏蛋白富含胶质脂蛋白和胆固醇，它被提倡参与蛋白质分选的膜结构域。众所周知，许多信号转导分子聚集在那里。不像作为另一个膜的微区域的Cabeole，区分捕获和鉴定过的脂筏蛋白比较困难，因为该蛋白在细胞膜上连续移动，同时也因为该蛋白质的大小和结构是不稳定的。因此，为了鉴别，需要特定的脂筏蛋白的标记。Flotillin作为脂筏蛋白的组成成分，被建议作为脂筏的标记物。
抗原：大鼠和小鼠Flotillin-1（SQVNHNKPLRTA）共同位点的C末端部分的合成肽
纯化：用抗原肽纯化
形式：冻干粉，含有0.05％NaN 3及1％BSA的PBS
使用方法：在该产品中加入1.0ml的去离子水（浓度为：100ug/ml）
稳定性：冻干粉，2-8℃可保存5年；溶液状态，-20℃可保存2年。
应用：该抗体可通过几种技术，例如亲和素生物素复合物（ABC），微波预处理后的包埋组织（10分钟，10mM柠檬酸缓冲液，pH值6.0），用于福尔马林固定石蜡进行免疫组化， 最优浓度约为2μg/ mL，但浓度应根据每个实验室进行优化。
该抗体可用于蛋白质印迹实验，浓度约为2μg/ mL。
特异性：与大鼠，小鼠和人类Flotillin-1反应（通过蛋白质印迹和免疫组织化学证实）
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Data Sheet
Code No. 18951
Anti-Mouse/Rat
Flotillin-1 (C) Rabbit IgG Affinity Purify
Introduction : Raft is rich in sphingo-gliycolipids ａnd cholesterol, it is advocated as a membrane domain which participates in protein sorting, ａnd it is known that many signal transduction molecules are gathering there. It is difficult to distinguish catch ａnd identify Raft protein, unlike Cabeole which is another micro domain of membrane, because the protein moves continuously on a cell membrane, ａnd because the size ａnd the structure of the protein are labile. For this reason, the marker specific to Raft was required in order to identify. Flotillin is the composition ingredient of Raft ａnd the possibility as a marker of Raft is suggested.
Antigen
: Synthetic peptide of the C-terminal part of the common site of rat ａnd mouse Flotillin-1 (SQVNHNKPLRTA)
Purification
Form
How to use
Stability
: Purified with antigen peptide
: Lyophilized product from 1 % BSA in PBS containing 0.05 % NaN3
: 1.0 mL deionized water will be added to the product (the conc. comes up 100 μg /mL).
: Lyophilized product, 5 years at 2 - 8 °C
: Solution, 2 years at –20 °C
Application: This antibody can be used for immunohistochemistry with formalin fixed paraffin embedded tissues after microwave pretreatment (10 min, 10mM Citrate Buffer, pH 6.0) by several techniques such as Avidin Biotin Complex (ABC) Method. The optimal concentration is about 2 μg/mL, however, the concentration should be optimized by each laboratory.
: This antibody can be used for western blotting in concentration of about 2 μg /mL.
Specificity: Reacts with rat, mouse ａnd human Flotillin-1 (Confirmed by western blotting and Immunohistochemistry)
第3年
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