【文献标题】Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply ａnd Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes
【作者】Longlong Li ,Mengling Peng ,Chongyang Ge, et.al
【作者单位】南京农业大学（Nanjing Agricultural University）
【文献中引用产品】
鸡糖原磷酸化酶(GP)ELISA试剂盒
鸡糖原合成酶(GS)ELISA试剂盒
鸡葡萄糖激酶(GK)ELISA试剂盒
鸡磷酸果糖激酶-1（PFK-1）ELISA试剂盒
鸡丙酮酸激酶（PK）ELISA试剂盒
鸡丙酮酸脱氢酶E1(PDH E1)ELISA试剂盒
鸡柠檬酸合成酶(CS)ELISA试剂盒
鸡乌头酸酶(ACO)ELISA试剂盒
鸡磷酸烯醇式丙酮酸羧激酶（PEPCK）ELISA试剂盒
鸡ATP-柠檬酸裂解酶(ACLY)ELISA试剂盒
鸡NADH脱氢酶ELISA试剂盒
鸡ATP合酶（ATP）ELISA试剂盒
【关键词】Hydroxycitric acid ? Acetyl-CoA ? ATP-citrate ? Energy metabolism ? Primary chicken hepatocytes
【DOI】 10.1159/000481564
【出版期刊】《CELLULAR PHYSIOLOGY AND BIOCHEMISTRY》
【产品原文引用】
Measurement of the key glucose metabolic enzyme protein content
Cells were cultured in 6-well plates (2×106 cells per well) ａnd treated with 0, 1, 10 or 50 μM (-)-HCA for 24 h. The cells were harvested ａnd disrupted ultrasonically in ice ａnd then centrifuged at 3000 rpm for 20 min at 4 °C. The supernatants were collected, the protein concentrations of glycogen phosphorylase (GP) (catalog #: E-75175), glycogen synthase (GS) (catalog #: E-75174), glucokinase (GK) (catalog #: E-76111), phosphofructokinase-1 (PFK-1) (catalog #: E-76131), pyruvate kinase (PK) (catalog #: E-76565), pyruvate dehydrogenase (PDH, E1) (catalog #: E-76118), citrate synthase (CS) (catalog #: E-75165), aconitase (ACO) (catalog #: E-75144) ａnd phosphoenolpyruvate carboxykinase (PEPCK) (catalog #: E-75117) were measured using ELISA kits according to the manufactures’ protocols (Shanghai Hengyuan Biological Technology Co., China). The enzyme protein content was normalized to total protein concentration in the sample.
第16年
在线咨询5分钟应答
