【文献标题】The sex steroid precursor dehydroepiandrosterone prevents nonalcoholic steatohepatitis by activating the AMPK pathway mediated by GPR30
【作者】Longlong Li , Hongjun Wang , Yao Yao , et.al
【作者单位】南京农业大学（Nanjing Agricultural University）
【文献中引用产品】
小鼠雌二醇(E2)ELISA试剂盒
小鼠睾酮(T)ELISA试剂盒
小鼠环磷酸腺苷(cAMP)ELISA试剂盒
小鼠肿瘤坏死因子α(TNF-α)ELISA试剂盒
小鼠白介素6(IL-6)ELISA试剂盒
【关键词】Dehydroepiandrosterone ,Biotransformation ,Nonalcoholic steatohepatitis ,AMP-Activated protein kinase ,G protein-coupled ,estrogen receptor
【DOI】https://doi.org/10.1016/j.redox.2021.102187
【出版期刊】《Redox Biology》
【产品原文引用】
Steroid hormone (E2 ａnd T) levels in HepG2 cells were measured by using the ELISA kits (Shanghai Hengyuan Biological Technology Co., Ltd, China), according to the manufacturer’s instructions.
Cyclic AMP (cAMP) levels in HepG2 cells ａnd BRL-3A cells were determined by ELISA kits (Shanghai Hengyuan Biological Technology Co., Ltd, China) following the manufacturer’s instructions.
After treatment, the cell culture supernatant of HepG2 cells ａnd BRL-3A cells was collected for testing. ELISA kits (Shanghai Hengyuan Biological Technology Co., Ltd, China) were used to determine the releases of TNF-α ａnd IL-6.
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