【文献标题】Reduced growth capacity of preimplantation mouse embryos in chronic unpredictable stress model
【作者】Xiaoli Zhao，Ruihong Ma，Xiaoyu Zhang，et.al
【作者单位】天津中医药大学第附属医院（ First Teaching Hospital of Tianjin University of Traditional Chinese Medicine）
【文献中引用产品】
小鼠促肾上腺皮质激素（ACTH）ELISA试剂盒
小鼠皮质酮/肾上腺酮（CORT）ELISA试剂盒
【关键词】chronic unpredictable stress model, differential gene expression, hypothalamus‐pituitary‐adrenal axis, preimplantation embryos, RNA sequencing
【DOI】DOI: 10.1002/mrd.23439
【影响因子(IF)】2.82
【出版期刊】《Molecular Reproduction ａnd Development》
【产品原文引用】
4.4 | Measurement of serum HPA axis‐associated
hormones
Blood was collected from 20 mice in each group by retro‐orbital bleeding. For serum preparation, the blood samples were left undisturbed at room temperature for 30 min, ａnd then subject to centrifugation at 1000g for 15 min at 4°C. The supernatants were transferred into new tubes ａnd stored at ?80°C until analysis. Serum levels of CRH, ACTH, and CORT were measured by ELISA according to manufacturer＇s manual (H‐20509, H‐20423, H‐20381, respectively; Shanghai Hengyuan Biotechnology Inc.).
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