【文献标题】Fluoxastrobin-induced effects on acute toxicity, development toxicity,oxidative stress, ａnd DNA damage in Danio rerio embryos
【作者】Cheng Zhang，Jingwen Zhang，Lusheng Zhu，et.al
【作者单位】山东农业大学（Shandong Agricultural University）
【文献中引用产品】
斑马鱼8羟基脱氧鸟苷（8-OHdG）elisa试剂盒
【关键词】96 h LC50，Delayed hatching，Teratogenicity，8-OHdG，AO-EB staining，Apoptosis
【DOI】https://doi.org/10.1016/j.scitotenv.2020.137069
【影响因子(IF)】5.92
【出版期刊】《Science of the Total Environment》
【产品原文引用】
2.7. Measurement of DNA damage induced by FLUO
To evaluate DNA damage to Danio rerio larvae induced by FLUO, 8-OHdG contents ａnd apoptosis were monitored. The supernatant was prepared from the homogenates prepared in Section 2.5 after centrifugation at 12,857g ａnd 4 °C for 10 min. The esultant solution was kept on ice during the entire procedure. A prior study (Guo et al., 2014)noted the significance of 8-OHdG in evaluating DNA damage. The 8-OHdH contents were monitored using an 8-OHdG ELISA kit (Hengyuan Biological Technology Co., Ltd., Shanghai, China) at 450 nm for 10 min (Multiskan MK3, Thermo Fisher Scientific, Massachusetts, USA) ａnd in units of ng/g protein. The total protein contents were assessed as in Section 2.6.As per Sun et al. (2019) ａnd Wang et al. (2018), apoptosis was monitored using acridine orange (AO)-ethidium bromide (EB) double dyeing kit (Solarbio Science & Technology Co., Beijing, China). At 96 hpf, ten larvae from each trial in Section 2.5 were rinsed twice with PBS (pH =7.4) ａnd dyed with 20 μL of AO ａnd EB working fluid (1/1, v/v) for 5 min ａnd then rinsed twice with PBS. Tritricaine (MS-222) was used for 3 min to anesthetize larvae for better observation. Then, the apoptosis of larvae was observed ａnd photographed under an inverted fluorescence microscope (xioVert. A1, Carl Zeiss AG, Oberkochen, Germany).
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