【文献标题】Novel ultrastructural findings on cardiac mitochondria of huddling Brandt＇s voles in mild cold environment
【作者】Zhe Wang，Jin-hui Xu，Jun-jie Mou，et.al
【作者单位】曲阜师范大学（Qufu Normal University）
【文献中引用产品】
田鼠三磷酸腺苷（ATP）elisa试剂盒
田鼠柠檬酸合成酶（CS）ELISA试剂盒
田鼠半胱氨酸蛋白酶3（caspase3）ELISA试剂盒
【关键词】huddling, apoptosis, mitochondrial fission, low temperature, heart, mitochondrial auhagy
【DOI】https://doi.org/10.1016/j.cbpa.2020.110766
【影响因子(IF)】4.0
【出版期刊】《Comparative Biochemistry ａnd Physiology, Part A》
【产品原文引用】
ATP synthase, CS, ａnd caspase3 activity
Samples stored at 80 °C were used to detect ATP synthase, citrate synthase, ａnd caspase3 activity. ATP synthase activity was determined by measuring free phosphate group at 450 nm using an ATP synthase Activity Assay Kit (H-172421, Shanghai Hengyuan Biological Technology Co., Ltd., China) according to the manufacturer’s instructions (OuYang et al., 2018).Citrate synthase activity was determined by measuring coenzyme A formation at 450 nm with a Citrate Synthase Activity Assay Kit (H-109821, Shanghai Hengyuan Biological Technology Co., Ltd., China) according to the manufacturer’s instructions (Song et al., 2018). Caspase3 activity in cell lysates was determined using a Caspase3 Activity Kit (BC3830, Solarbio, Beijing, China) following the manufacturer’s protocols (Xu et al., 2018).
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