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The CRL-1621cell line is reported to be positive for Epstein-Barr nuclear antigen （EBNA +） ａnd Epstein-Barr viral capsid antigen （EBVCA +）. Since the HMy2.CIR cell line was derived from the HMy.2 B lymphoblastoid cell line, a fast growing mutant of the ARH⁻⁷7 cell line （ATCC CRL-1621）, it is assumed to be EBNA positive. The HMy2.CIR cell line was derived from the HMy.2 B lymphoblastoid cell line, a fast growing mutant of the ARH⁻⁷7 cell line （ATCC CRL-1621）, by gamma irradiation ａnd selection for loss of HLA class I antigen expression. [23312] The cells express no HLA A or B locus products, but do express small amounts of HLA Cw4. The cells are useful as host for transfected class I major histocompatibility antigen genes.
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove＇s Modified Dulbecco＇s Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide （CO2）, 5% Temperature: 37.0°C
Subculturing:
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10（5） viable cells/ml. Interval: Maintain between 2 X 10（5） ａnd 1 X 10（6） viable cells/ml. Medium Renewal: Every 2 to 3 days
Preservation:
Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO Storage temperature: liquid nitrogen vapor phase
23312: Storkus WJ, et al. Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes. Proc. Natl. Acad. Sci. USA 86: 2361-2364, 1989. PubMed: 2784569
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