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AAT XFD488 tyramide reagent *Same Struct

点击次数：475

价格：￥电议

更新日期：2026/9/8 5:11:04

生产地：中国大陆

产品型号：11070

简单介绍：XFD488 tyramide reagent *Same Structure to Alexa Fluor™ 488 tyramide*

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相关标签：11070,,XFD488,tyramide,reagent

详细内容

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XFD488 is manufactured by AAT Bioquest, ａnd it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). For many immunohistochemical (IHC) applications, the traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit the sensitivity ａnd utility of these procedures. Tyramide signal amplification (TSA) has proven to be a particularly versatile ａnd powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. To achieve maximal IHC detection, tyramine is prelabeled with a fluorophore. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label translates ultrasensitive detection of low-abundance targets ａnd the use of smaller amounts of antibodies ａnd hybridization probes. In immunohistochemical applications, sensitivity enhancements derived from TSA method allow primary antibody dilutions to be increased to reduce nonspecific background signals, ａnd can overcome weak immunolabeling caused by suboptimal fixation procedures or low levels of target expression. XFD488 tyramide contains the bright XFD488 dye that can be readily detected with the standard FITC filter set.

Platform

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	FITC filter set

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 tyramide reagent *Same Structure to Alexa Fluor™ 488 tyramide* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	116.82 µL	584.099 µL	1.168 mL	5.841 mL	11.682 mL
5 mM	23.364 µL	116.82 µL	233.639 µL	1.168 mL	2.336 mL
10 mM	11.682 µL	58.41 µL	116.82 µL	584.099 µL	1.168 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

Open in: Advanced Spectrum Viewer

Cursor Location

Wavelength (nm)	692.18
Relative Intensity (%)	28.18

References

View all 74 references: Citation Explorer

Enhanced detection of Porcine reproductive ａnd respiratory syndrome virus in fixed tissues by in situ hybridization following tyramide signal amplification
Authors: Trang NT, Hirai T, Ngan PH, Lan NT, Fuke N, Toyama K, Yamamoto T, Yamaguchi R.
Journal: J Vet Diagn Invest (2015): 326

Tyramide Signal Amplification for Immunofluorescent Enhancement
Authors: Faget L, Hnasko TS.
Journal: Methods Mol Biol (2015): 161

KSHV cell attachment sites revealed by ultra sensitive tyramide signal amplification (TSA) localize to membrane microdomains that are up-regulated on mitotic cells
Authors: Garrigues HJ, Rubinchikova YE, Rose TM.
Journal: Virology (2014): 75

Multiplexed immunohistochemistry, imaging, ａnd quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging ａnd multiplex analysis
Authors: Stack EC, Wang C, Roman KA, Hoyt CC.
Journal: Methods (2014): 46

Rapid ａnd sensitive detection of Escherichia coli O157:H7 in milk ａnd ground beef using magnetic bead-based immunoassay coupled with tyramide signal amplification
Authors: Aydin M, Herzig GP, Jeong KC, Dunigan S, Shah P, Ahn S.
Journal: J Food Prot (2014): 100

Sensitive whole-mount fluorescent in situ hybridization in zebrafish using enhanced tyramide signal amplification
Authors: Lauter G, Soll I, Hauptmann G.
Journal: Methods Mol Biol (2014): 175

Characterization of GABAergic neurons in the mouse lateral septum: a double fluorescence in situ hybridization ａnd immunohistochemical study using tyramide signal amplification
Authors: Zhao C, Eisinger B, Gammie SC.
Journal: PLoS One (2013): e73750

Quantification of alpha-tubulin isotypes by sandwich ELISA with signal amplification through biotinyl-tyramide or immuno-PCR
Authors: Draberova E, Stegurova L, Sulimenko V, Hajkova Z, Draber P.
Journal: J Immunol Methods (2013): 63

Development of a near-infrared fluorescence ELISA method using tyramide signal amplification
Authors: Gong H, Cradduck M, Cheung L, Olive DM.
Journal: Anal Biochem (2012): 27

Integrated tyramide ａnd polymerization-assisted signal amplification for a highly-sensitive immunoassay
Authors: Yuan L, Xu L, Liu S.
Journal: Anal Chem (2012): 10737

Documents

Safety data sheet
Product Datasheet
Certificate of Analysis

Application notes

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FAQ

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11070,,XFD488,tyramide,reagent