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小鼠β2糖蛋白1抗体IgA(β2-GP1 IgA/G/M)ELISA试剂盒
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上海娜连生物销售各种ELISA试剂盒,有RD,RB原装,标本齐,提供代测。如有疑问,可与上海娜连生物销售或技术联系 负责人:王经理。
产品名称:小鼠β2糖蛋白1抗体IgA(β2-GP1 IgA/G/M)ELISA试剂盒
英文名称:Mouse β2-glycoprotein 1 antibody IgA/G/M,β2-GP1 IgA/G/M ELISA KIT
产品规格:96人份/48人份
各种种属:人、大小鼠、豚鼠、兔子、猪、犬、牛、羊…
标本齐:血清、血浆、细胞培养上清液、组织匀浆、组织液、关节液、胸腔溢液等…
保存条件:2-8℃
联系电话:021-6051 7348,138 1714 0470
提供ELISA代做实验 在我司购买试剂盒提供免费代测
Assay procedure
1. Dilute and add sample:Dilute Original density Standard as follow table:
| 160ng/L | 5 Standard | 150μl Original density Standard+150μl Standard diluent |
| 80ng/L | 4 Standard | 150μl 5 Standard+150μl Standard diluent |
| 40ng/L | 3 Standard | 150μl 4 Standard+150μl Standard diluent |
| 20ng/L | 2 Standard | 150μl 3 Standard +150μl Standard diluent |
| 10ng/L | 1 Standard | 150μl 2 Standard +150μl Standard diluent |
2.add sample:Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold(or 20-fold) wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing:Uncover Closure plate membrane, discardLiquid, dry by swing, add washing buffer to every well, still for 30s then drain,repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
“小鼠β2糖蛋白1抗体IgA(β2-GP1 IgA/G/M)ELISA试剂盒”含税含运费,提供代测,中英文双版说明书,品牌美国RD、RB、IBL、Omega,质量,详情请来电向我司索取信息。
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