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人单核细胞白血病THP-1FH0112
价格：￥电议
品牌名称：富衡型号：FH0112 原产地：中国大陆 更新时间：2026/11/8 9:11:52
产品摘要：THP-1 细胞系源自一名 1 岁墨西哥裔男性急性单核细胞白血病（AML-M5 型）患者的外周血，该患者处于疾病阶段。急性单核细胞白血病属于髓系白血病的一种，其特征是骨髓和外周血中单核细胞异常增殖，患者常表现为贫血、感染及出血倾向。
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详细内容
| 产品名称 | 人单核细胞白血病；THP-1 (STR) |
|---|---|
| 品牌 | FuHeng |
| 英文名 | THP-1 (STR) |
| 货号 | FH0112 |
| 规格 | 1×10⁶cells/T25培养瓶 |
| 价格 | 1500.0 |
| 培养 | RPMI1640+0.05mM+10%FBS |
| 别称 | THP1; THP 1; THPI; THP-1(ATCC); THP-1-O; O-THP-1; Tohoku Hospital Pediatrics-1 |
| 形态特征 | 单核细胞 |
| 生长特性 | 悬浮生长 |
| 培养条件 | 气相：空气，95%；CO2,5%温度：37℃ |
| 传代方法 | 维持细胞浓度在2~4×105~8×105/ml，勿超过1×106/ml；2~3天换液1次。 |
| 组织来源 | 外周血 |
| 细胞简介 | HP-1 细胞系源自一名 1 岁墨西哥裔男性急性单核细胞白血病（AML-M5 型）患者的外周血，该患者处于疾病阶段。急性单核细胞白血病属于髓系白血病的一种，其特征是骨髓和外周血中单核细胞异常增殖，患者常表现为贫血、感染及出血倾向。 |
| 特征特性 | 该细胞从一名1岁的患有急性单核细胞性白血病的男孩的外周血中分离建立。该细胞可以吞噬乳胶颗粒和激活的红细胞，细胞膜和胞浆内均没有免疫球蛋白，表达C3R和FcR；可受佛波酯TPA诱导向单核系方向分化；可作为转染宿主。 |
| 培养基 | THP-1完全培养基 |
T25 细胞到货处理
观察：
1、收到细胞后，请及时核对培养瓶上标注的细胞名称是否与订购的细胞名称一致以及培养瓶是否有破损或漏液等异常情况。
处理：
1、75%酒精棉球擦拭 T25 细胞培养瓶外部。
2、显微镜观察细胞生长情况，并对细胞进行不同倍数拍照保存（40x,100x,200x 各一张）前三天照片为重要售后依据，不提供照片默认收到状态良好。
3、不要打开培养瓶盖，将细胞放入 37 度培养箱中静置 3-4 小时后再做处理，以稳定细胞状态。
4、收到细胞后，及时查看说明书是贴壁细胞还是悬浮细胞形态，并按常规贴壁或悬浮细胞的传代方法操作。
冻存细胞到货处理
1、收到细胞后，检查外包装情况和箱内是否还有干冰。如有外包装破损干冰已完全挥发等问题，请即时联系。
2、正常请进行以下操作：将细胞取出转移至液氮或－80 度冰箱保存，建议尽早复苏。
3、复苏管如有活性状态问题及时与我们联系，会有技术人员与您沟通指导后再复苏第二管。特别说明：未与我方联系擅自复苏第二管出现问题不予售后。
细胞复苏
1、 从液氮中取出细胞冻存管（注意：佩戴防爆管面具），快速将其置入37℃水浴中解冻，直至冻存管中无结晶，然后用 75%的酒精擦拭冻存管外壁；
2、将冻存管中的细胞移至含 5ml 完全培养基的 15ml 离心管中，1000rpm 离心5min；
3、弃上清，沉淀用 5ml 完全培养基重悬，接种 T25 培养瓶，于 37℃,5%CO2 细胞培养箱中培养；
4、第二天，换用新鲜完全培养基继续培养。
细胞传代
1、细胞生长至覆盖培养瓶的 80%面积时，弃 T25 培养瓶中的培养液，用PBS 清洗细胞一次：
2、添加 0.25%胰蛋白酶消化液约 1ml 至培养瓶中，倒置显微镜下观察，待细胞回缩变圆后加入 5ml 完全培养液终止消化，再轻轻吹打细胞使之脱落，然后将悬液转移至15ml 离心管中，1000rpm 离心 5min；
3、弃上清，沉淀细胞用 1-2ml 完全培养基重悬，然后按 1:2 比例进行分瓶传代，补充新的完全培养基至 5-8ml/瓶，放入 37℃,5%CO2 细胞培养箱中培养；
细胞冻存
1、细胞生长至覆盖培养瓶的 80%面积时，弃 T25 培养瓶中的培养液，用PBS 清洗细胞一次；
2、添加 0.25%胰蛋白酶消化液约 1ml 至培养瓶中，倒置显微镜下观察，待细胞回缩变圆后加入 5ml 完全培养液终止消化，再轻轻吹打细胞使之脱落，然后将悬液转移至15ml 离心管中，1000rpm 离心 5min；
3、 弃上清，沉淀细胞加入 1ml/支的富衡无血清冻存液（FH7001），混匀后加入冻存管中。（如：冻一支，加入 1ml 无血清冻存液）
4、 将冻存细胞直接放入－80℃冰箱即可；如后期要将细胞转入液氮罐中，需在-80℃冰箱存放 24 小时以上。
本库的细胞系（株）仅用于科研工作。
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热门标签：人单核细胞白血病,THP-1,THP-1细胞
