吲哚菁绿（ICG）是一种用于医学研究的花青染料。它用于确定心输出量，肝功能和肝血流量，以及用于眼科血管造影。它具有接近800nm的峰值光谱吸收。这些红外线能穿透视网膜层，使ICG血管造影成像比荧光血管造影更深的循环模式。ICG的半衰期为150至180，仅通过肝脏从胆汁液中排出。近的一项研究表明ICG在注射后20分钟内靶向动脉粥样硬化，并提供足够的信号增强，用于动脉粥样硬化兔子体内的脂质，发炎，冠状动脉大小的斑块的检测。离体荧光反射成像显示，与注射生理盐水的动脉粥样硬化兔相比，注射ICG的动脉粥样硬化兔的斑块靶 - 背景比高。氨基反应性ICG衍生物用于与抗体和其他生物分子制备ICG生物缀合物。所有ICG染料都需要溶解在DMSO中。
英文名称：ICG-Maleimide，ICG-Mal
中文名称：吲哚菁绿-马来酰亚胺
CAS：2143933-81-5
分子量：C51H56N4O6S
分子式：853.09
Excitation (nm) 789
Emission (nm) 814
供应商：陕西新研博美生物科技有限公司实验室出品
PREPARATION OF STOCK SOLUTIONS：
Unless otherwise noted, all unused stock solutions should be divided intosingle-use aliquots ａnd stored at -20 °C after preparation. Avoid repeatedfreeze-thaw cycles.
1. ICG maleimide stock solution (Solution B)Add anhydrous DMSO into the vial of ICG maleimide to make a 10 mM stocksolution. Mix well by pipetting or vortex. Note: Prepare the dye stock solution(Solution B) before starting the conjugation. Use promptly. Extended storage ofthe dye stock solution may reduce the dye activity. Solution B can be stored infreezer for upto 4 weeks when kept from light ａnd moisture. Avoid freeze-thawcycles.
2. Protein stock solution (Solution A)Mix 100 µL of a reaction buffer (e.g., 100 mM MES buffer with pH ~6.0) with 900µL of the target protein solution (e.g. antibody, protein concentration >2 mg/mL ifpossible) to give 1 mL protein labeling stock solution. Note: The pH of the proteinsolution (Solution A) should be 6.5 ± 0.5. Note: Impure antibodies or antibodiesstabilized with bovine serum albumin (BSA) or other proteins will not be labeledwell. Note: The conjugation efficiency is significantly reduced if the proteinconcentration is less than 2 mg/mL. For optimal labeling efficiency the finalprotein concentration range of 2-10 mg/mL is recommended.
3. Optionalif your protein does not contain a free cysteine, you must treat your protein withDTT or TCEP to generate a thiol group. DTT or TCEP are used for converting adisulfide bond to two free thiol groups. If DTT is used you must remove free DTTby dialysis or gel filtration before conjugating a dye maleimide to your protein.Following is a sample protocol for generating a free thiol group:1. Prepare a fresh solution of 1 M DTT (15.4 mg/100 µL) in distilledwater.2. Make IgG solution in 20 mM DTT: add 20 µL of DTT stock per ml ofIgG solution while mixing. Let stand at room temp for 30 minuteswithout additional mixing (to minimize reoxidation of cysteines tocystines).3. Pass the reduced IgG over a filtration column pre-equilibrated with"Exchange Buffer". Collect 0.25 mL fractions off the column.4. Determine the protein concentrations ａnd pool the fractions with themajority of the IgG. This can be done either spectrophotometrically orcolorimetrically.5. Carry out the conjugation as soon as possible after this step (seeSample Experiment Protocol). Note: IgG solutions should be >4mg/mL for the best results. The antibody should be concentrated ifless than 2 mg/mL. Include an extra 10% for losses on the bufferexchange column. Note: The reduction can be carried out in almostany buffers from pH 7⁻⁷.5, e.g., MES, phosphate or TRIS buffers.Note: Steps 3 ａnd 4 can be replaced by dialysis.
陕西新研博美吲哚菁绿其他相关化合物：
吲哚菁绿ICG-Sulfo-Osu
吲哚菁绿ICG-ATT
吲哚菁绿ICG-Osu
吲哚菁绿ICG-Sulfo-EG4-Osu
吲哚菁绿ICG-Sulfo-EG8-Osu
吲哚菁绿ICGamine
吲哚菁绿lCGamine
吲哚菁绿ICGacid
吲哚菁绿ICG-PEG12-Osu
吲哚菁绿ICG Xtra-Osu
吲哚菁绿ICGmaleimide
