膳食纤维检测试剂盒K-TDFR-100A

For the determination of Total Dietary Fiber in cereal products,
foodstuffs, feeds ａnd other materials
Principle:
(α-amylase + amyloglucosidase)
(1) Starch + H2O → D-glucose

膳食纤维检测试剂盒K-TDFR-100A(protease)
(2) Protein + H2O → peptides

(3) Dietary fiber determined gravimetrically following alcohol
precipitation

(4) Ash ａnd residual protein determined on DF residues
and subtracted
Kit size: 100 / 200 assays Method: Hydrolysis / removal of non-dietary

fibre components Total assay time: ~ 100 min Detection limit: 0.5-100% of sample weight Application examples: Food ingredients, food products ａnd other materials Method recognition: AOAC (Methods 985.29, 991.42, 991.43 ａnd 993.19), AACC (Methods 32-05.01, 32-06.01, 32-07.01 ａnd 32-21.01) ａnd CODEX (Type I Method)

Total Dietary Fiber Assay Kit, for the measurement ａnd analysis of total, soluble ａnd insoluble dietary fiber according to AOAC ａnd AACC approved methods. See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998.

Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble ａnd insoluble polysaccharides ａnd non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins ａnd lignins. The procedures for the determination ａnd analysis of total dietary fiber as outlined in our booklet are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 ａnd AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 ａnd AACC Method 32-06.01.

膳食纤维检测试剂盒K-TDFR-100A1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399.

2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370.

3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.

Two separate methods are described in the associated data booklet:

METHOD 1:

DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER

Based on AOAC Method 991.43 “Total, Soluble, ａnd Insoluble Dietary Fiber in Foods” (First Action 1991) ａnd AACC Method 32-07.01 “Determination of Soluble, Insoluble, ａnd Total Dietary Fiber in Foods ａnd Food Products” (Final Approval 10-16-91).

METHOD 2:

膳食纤维检测试剂盒K-TDFR-100ADETERMINATION OF TOTAL DIETARY FIBER

Based on AACC method 32-05.01 ａnd AOAC Method 985.29.