Tris Buffer pH 10.0 （10x）

Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded （FFPE） tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens （cellular, membrane ａnd nuclear） by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 10 antigen retriever solution.
Preparation of Working Solution: Dilute this 10x buffer as needed （e.g. 90 ml of deionized or distilled water + 10 ml of this buffer）, mix well, 1x buffer can be stored at 2-8°C.