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文献引用产品：人正常前列腺上皮细胞RWPE-1
点击次数：822发布时间：2025/12/17 16:28:32
文章标题：The Expression of miR-205 in Prostate Carcinoma and the Relationship with Prognosis in Patients
期刊：Computational and Mathematical Methods in Medicine
作者列表：Zhuifeng Guo, Xuwei Lu, Fan Yang, Liang Qin, Ning Yang, Peiran Cai, Conghui Han, Jiawen Wu, Hang Wang
发表时间：2022-8-30
影响因子：2.809
DOI:10.1155/2022/1784791
主要研究成果：Abstract
Objectives
To analyze the expression, biological function of branched chain amino-acid transaminase 1 (BCAT1) in oral squamous cell carcinoma (OSCC).
Materials and Methods
Real-time PCR and immunohistochemistry were used to analyze the expression of BCAT1 protein in OSCC and normal oral tissues. Based on the clinicopathological information of patients, the relationship between the expression of BCAT1 protein and other clinicopathological factors was analyzed. Real-time PCR and western blot assays were used to analyze the expression of BCAT1 gene and protein in normal human oral keratinocytes (HOK) and human OSCC cells, respectively. After BCAT1 overexpression or knockdown, the proliferation, cell cycle, migration, and invasion of human OSCC cells were analyzed by CCK8, flow cytometry, wound healing, and transwell invasion assays, respectively. After adding the BCAT1 inhibitor EGR240 to OSCC cells, the changes in cell proliferation, migration, and invasion ability in OSCC cells were analyzed. Based on the TCGA database, the involved signal pathway in BCAT1-related and BCAT1-binding genes was obtained for Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, verified by western blot assays. After inhibiting PI3K, the effect of BCAT1 on the expression of the downstream phosphorylated protein of the PI3K-Akt signaling pathway was analyzed by western blot assays. The relationship between the expression of BCAT1 and EMT-related protein of OSCC cells was also analyzed.
