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PC-3M人前列腺癌细胞鉴定报告
点击次数：1414发布时间：2019/5/5 9:01:18
STR Profiling Report
Sample From: EK-Bioscience
Sample Type: Cell Line
Testing Method: STR Genotyping
Report Time: 12/20.2018
Cell Line Authentication – STR Profiling Report
Sample code
Table 1. Sample Code
| Customer’s code | Company Code |
| PC-3M | 20181210-01 |
Sample Number:1
Sample Type: Cell line
Testing Type: STR
Testing Method:
DNA was extracted by a commercial kit from CORNING (AP-EMN-BL-GDNA-250G). The twenty STRs including Amelogenin locus were amplified by six multiplex PCR ａnd separated on ABI 3730XL Genetic Analyzer. The signals were then analyzed by the software GeneMapper.
Data Interpretation:
Cell lines were authenticated using Short Tandem Repeat (STR) analysis asdescribed in 2012 in ANSI Standard (ASN-0002) by the ATCC Standards Development Organization (SDO) ａnd in Capes-Davis et al., Match criteria for human cell line authentication: Where do we draw the line? Int J Cancer.2013;132(11):2510-9.
Test Results:
1. STR profile
Table 2. STR ａnd Amelogenin Genotyping Results of Cell line 20181210-01.
| Loci | Sample information | Cell Bank information | ||||
| Sample name：PC-3M | Cell line name：PC-3M | |||||
| Allele1 | Allele2 | Allele3 | Allele1 | Allele2 | Allele3 | |
| D5S818 | 13 | 13 |
| 13 | 13 |
|
| D13S317 | 11 | 11 |
| 11 | 11 |
|
| D7S820 | 8 | 11 |
| 8 | 11 |
|
| D16S539 | 11 | 11 |
|
|
|
|
| VWA | 17 | 17 |
| 17 | 17 |
|
| TH01 | 6 | 7 |
| 6 | 7 |
|
| AMEL | X | X |
| X | X |
|
| TPOX | 8 | 9 |
| 8 | 9 |
|
| CSF1PO | 11 | 11 |
| 11 | 11 |
|
| D12S391 | 21 | 21 |
|
|
|
|
| FGA | 24 | 24 |
| 24 | 24 |
|
| D2S1338 | 18 | 20 |
|
|
|
|
| D21S11 | 29 | 31.2 |
|
|
|
|
| D18S51 | 14 | 15 |
|
|
|
|
| D8S1179 | 13 | 13 |
|
|
|
|
| D3S1358 | 16 | 16 |
| 16 | 16 |
|
| D6S1043 | 18 | 18 |
|
|
|
|
| PENTAE | 10 | 17 |
|
|
|
|
| D19S433 | 14 | 14 |
|
|
|
|
| PENTAD | 9 | 9 |
|
|
|
|
| D1S1656 | 12 | 16 |
|
|
|
|
2. database annotation
Figure 1. STR matching analysis
Note: The STR online match analysis of the test cell against EXPASY database, showing cell number (Cell No.CVCL_9555) ａnd cell name.
3. Authentication
The submitted profile is similar to the following DSMZ human cell line: /.
l Note: A cell line can be considered to be authenticated when 80% (exact match) of the alleles in its STR profile match profiles from tissue or other cell line samples from that donor or from database. Cell lines with between a 55% to 80% (similar) match require further profiling for investigation of relatedness.
Appendix:
1. Genotyping Strategy ａnd Site Distribution
Table S1. Experimental Strategy ａnd Sites
|
| Strategy 1 | Strategy 2 | Strategy 3 | Strategy 4 |
| 1 | D3S1358 | D8S1179 | D19S433 | AMEL |
| 2 | VWA | D21S11 | TH01 | D1S1656 |
| 3 | D7S820 | D16S539 | D13S317 | D5S818 |
| 4 | CSF1PO | D2S1338 | TPOX | D12S391 |
| 5 | PENTAE | PENTAD | D18S51 | FGA |
| 6 |
|
| D6S1043 |
|
The allele match algorithm compares the 8 core loci plus amelogenin only, even though alleles from all lociwill be reported when available.
2. DSMZ tools was used to carry on the cell line comparison, which contains 2455 cell lines STR data from ATCC, DSMZ, JCRB ,ECACC，GNE and RIKEN databases. If the cell is not included in the above cell library, users need to compared with other databases.
Technician: Jianan Zhang
Checked by: Ning Qian
Issued by: Yang Bai
Issue date:5/5/2019
