hEEC 人子宫内膜上皮细胞Product Format: a T25 flask
Culture Properties：贴壁
Complete Growth Medium: 89%EMEM(MEM+NEAA)+10%FBS+1%双抗
Application: Cells ａnd cancer research
NOTE: FOR RESEARCH USE ONLY.
Components
Item Specifications
a T25 flask 2E6 cells
Manual 1 copy
Operation steps for flask
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Subculturing Volumes are given for a T25 flask. Corning T25 flasks (catalog #430168)
are recommended for subculturing this product. Increase or decrease the
amount of dissociation medium needed proportionally for culture vessels of
other sizes.
1. Remove ａnd discard culture medium.
2. Briefly rinse the cell layer with 2 to 3 mL of PBS solution to remove all
traces of serum that contains trypsin inhibitor.
3. Add 1.0 mL of Trypsin-EDTA solution to flask ａnd observe cells under
an inverted microscope until cell layer is dispersed.
Note: To avoid clumping do not agitate the cells by hitting or shaking
the flask while waiting for the cells to detach.To avoid cell death do not
Digesting the cells to float.
4. Add 6.0 to 8.0 mL of complete growth medium ａnd aspirate cells by
gently pipetting.
5. Centrifuge cells at 150rpm for 3 minutes. Discard the supernatant and
add fresh medium,resuspend the cells gently.
6. Subculture when cell concentration is between 6 ａnd 7 x 10*4 cells/cm2.
Supplement sufficient medium for culture cells.
Subcultivation Ratio: 1:2 to 1:4
Medium Renewal: Every 2 to 3 days
Cryopreservation Freeze medium: Complete growth medium supplemented with 8% (v/v)
DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
