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技术文章
文献引用产品：植物过氧化物酶（POD）Elisa试剂盒
点击次数：1030 发布时间：2026/3/27 17:04:30
文章标题：VaAPL1 Promotes Starch Synthesis to Constantly Contribute to Soluble Sugar Accumulation, Improving Low Temperature Tolerance in Arabidopsis ａnd Tomato
作者列表：Guoping Liang, Yanmei Li, Ping Wang, Shuzhen Jiao, Han Wang, Juan Mao, Baihong Chen
发表时间：2022-6-22
期刊：Frontiers in Plant Science
影响因子：6.627
DOI:10.3389/fpls.2022.920424
Abstract
ADP-glucose pyrophosphorylase （AGPase） is a key rate-limiting enzyme involved in starch synthesis. APL1, an AGPase large subunit, plays an important role in the growth ａnd development of grapes; however, its function in withstanding low temperature （LT） remains elusive. Hence, VaAPL1 was cloned from Vitis amurensis （Zuoshan I）, ａnd its function was characterized. The gene was highly expressed in the phloem of V. amurensis during winter dormancy （0, −5, and − 10°C）. Phylogenetic relationships demonstrated that VaAPL1 was closely genetic related to SlAPL1 （from Solanum lycopersicum）, ａnd clustered into I group. Further, VaAPL1 was ectopically expressed in Arabidopsis thaliana （ecotype Columbia, Col） ａnd tomato （“Micro-Tom” tomato） to characterize its function under LT. Compared with Col, the average survival rate of VaAPL1-overexpressing A. thaliana exceeded 75.47% after freezing treatment. Moreover, reactive oxygen species （ROS） content decreased in VaAPL1-overexpressing A. thaliana ａnd tomato plants under LT stress. The activities of AGPase, ａnd starch contents in VaAPL1-overexpressing A. thaliana were higher than in Col after LT stress. The contents of sucrose ａnd glucose were accumulated in overexpressing plants compared with wild-type at 0 h ａnd 24 h after LT stress. Tranｓcriptome sequencing of overexpressing tomato plants revealed involvement in sugar ｍmbedbolism ａnd the hormone signal pathway, ａnd Ca2+ signaling pathway-related genes were up-regulated. Hence, these results suggest that overexpression of VaAPL1 not only ensured sufficient starch converting into soluble sugars to maintain cell osmotic potential ａnd provided energy, but also indirectly activated signal pathways involved in LT to enhance plant tolerance.
Keywords: grape, VaAPL1, low temperature, Arabidopsis thaliana, tomato, tranｓcriptome
原创作者：上海雅吉生物科技有限公司
