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技术文章
文献引用产品：番茄斑萎病毒（TSWV）Elisa试剂盒
点击次数：1032 发布时间：2026/3/25 15:43:33
文章标题：Targeting phase separation of TSWV nucleocapsid protein with novel methyl vanillate derivative A10 to inhibit biomacromolecule aggregate ｆormation
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL
作者列表：Qiangsheng Ge, Yanju Wang, Ziming Wang, Yuyuan Yang, Tangbing Yang, Chunni Zhao, Deyu Hu, Runjiang Song
发表时间：2026-2-22
影响因子：8.5
DOI:10.1016/j.ijbiomac.2026.151040
主要研究成果：Abstract
Phase separation (PS)-driven biomolecular condensates have recently emerged as crucial elements in the life cycles of numerous phytoviruses and their interactions with host plants. While inhibiting the ｆormation of these aggregates presents a promising plant protection strategy, research into discovering such inhibitors remains scarce. Here, we report a novel achievement in this field. Through biological screening of a series of structurally designed methyl vanillate derivatives incorporating N-Acetyl-D-glucosamines, a novel compound A10 is discovered to possess excellent antiviral potency against tomato spotted wilt virus (TSWV). Compared with the commercial control ribavirin, A10 demonstrates superior curative (56.9%), protective (50.5%), and inactivating (62.2%) activities, with its EC50 based on inactivation effect (181.7 μg/mL) being lower than that of ribavirin (EC50 = 748.5 μg/mL). A10 treatment significantly reduces the ｆormation of TSWV nucleocapsid (N) protein condensates. Molecular docking, Molecular dynamics simulations, and Bio-ｌayer interferometry analyses identify Arg94, Tyr184, and Lys192 as key residues on the N protein for A10 binding. Combining Agrobacterium-mediated infiltration assays and confocal microscopy observations allows A10 to mechanistically inhibit PS and aggregate generation of the N protein, thereby significantly suppressing viral RNP proliferation and systemic infection in host plants. This study contributes a valuable new lead compound for phytoviral management by targeting PS.
原创作者：上海雅吉生物科技有限公司
