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文献引用产品：人冠状动脉内皮细胞HCAEC
点击次数：1062 发布时间：2026/3/19 16:41:51
文章标题：DDIAS Regulation of STAT3/CCL2 Promotes Macrophage Polarization to M1 type in Kawasaki Disease
作者列表：Yiping Yu, Xiujuan Xu
期刊：ANNALS OF CLINICAL AND LABORATORY SCIENCE
发表时间：2024-7-1
影响因子：1.1
DOI:PMID:39293834
主要研究成果：Abstract
Objective To investigate the molecular mechanism by which DNA damage induced apoptosis suppressor (DDIAS) regulates STAT3/CCL2 to enhance macrophage polarization to M1 type in Kawasaki disease (KD).
Methods A KD vascular model was established by culturing human coronary artery endothelial cells (HCAECs) in vitro. Small interfering RNA of DDIAS (si-DDIAS) was transfected into the KD cell model. The human macrophage cell line THP-1 was induced into M1 macrophages using phorbol myristate acetate (PMA) and lipopolysaccharide (LPS) and co-cultured with the endothelial cells using the HCAECs medium. Western blot analysis was utilized to assess cellular DDIAS, p-STAT3, STAT3, and CCL2 protein expression. MTT was utilized to detect cell proliferation. ELISA was utilized to assess the expression levels of TNF-α, IL-4, IL-6, IL-8 and CCL2 in cell supernatants. Flow cytometry was utilized to examine cell apoptosis and the expression of M1 macrophage surface marker CD86.
原创作者：上海雅吉生物科技有限公司
