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技术文章
Live/Dead Cell Staning Kit
点击次数：3972 发布时间：2019/11/11 9:26:11
The ScienCell™ Live/Dead Cell Staining Kit is a two-color fluorescence assay for convenient
discrimination of live from dead animal cells, which can be applied to fluorescence microscope, fluorescent plate
reader ａnd flow cytometer. Due to changes in membrane integrity, live cells appear fluorescent green ａnd dead
cells, fluorescent red.
Kit Components
Cat. No. # of vials Name Quantity Storage
8138 1 Live/Dead Cell Staining
Solution (100×) 0.1 ml -20ºC, dark
Quality Control
The ScienCell™ Live/Dead Cell Staining Kit is applied to freshly harvested human endothelial cells by
trypsin/EDTA, ａnd resulting fluorescence micrograph is shown in Figure 1. Live cells appear fluorescent green
and dead cells, fluorescent red.
Procedures
A. Preparation of working staining solution:
1. Thaw ａnd dilute appropriate volume of Live/Dead Cell Staining Solution Stock (100×) 100
times with PBS to make the working staining solution, which is stable for up to one month at
4ºC.
B. Staining of cells:
1. Collect cells (1~2×10
6
/ml) in PBS.
2. Add equal volume of working staining solution to cell suspension (e.g. 10 µl), mix well.
C. Observation:
1. Place the mixture on a microscope slide, ａnd cover it with a glass cover slide.
2. Observe cells immediately under a fluorescence microscope. Live cells should stain
fluorescing
fluorescing
green (Ex495/Em518), while dead cells should appear red (Ex493/Em620).
3. Count cells ａnd calculate the live to dead ratio.
1Figure 1. A fluorescence micrograph showing endothelial cells stained with SicenCell™ Live/Dead Cell Staining
Kit, with 30 live cells appearing fluorescing green ａnd 2 dead cells appearing red.
原创作者：上海雅吉生物科技有限公司
