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技术文章
人血管生成定量PCR芯片试剂盒
点击次数：3666 发布时间：2019/6/6 10:23:02
人血管生成定量PCR芯片试剂盒描述：
ScienCell＇s GeneQuery™ Human Angiogenesis qPCR array kit (GQH-ANG) is designed to facilitate gene expression profiling of 88 key genes involved in angiogenesis during differing environmental conditions based on extensive literature research. Brief examples of how included genes may be grouped according to function or condition are shown below:
by stages of angiogenesis:
Sprouting: LGALS9, DLL4, NOTCH1, FLT1, KDR
Migration: FGF1, PECAM1, CD82, ITGB1, NRP1
ECM remodeling: MMP2, MMP9, ITGAV, LAMA5, NRP1
Maturation: ANGPT1, VCAM1, PDGFRB, FOS, SELE
Permeability: CDH2, CDH5, ANGPT2, EDN1, KLF2
by environmental conditions:
Hypoxia: HIF1A, NOS3, EGLN1, PDGFRA, ITGA5
Inflammation: EPHA2, IL1B, PTGS2, TNFAIP1, CCL2
Wound healing: FGF2, ID1, ITGB3, COL4A3, PLAU
Coagulation: CNTN1, CD36, SERPINE1, FN1, THBS1
Signal regulation: EFNB2, BMP1, MEOX2, HHEX, VEGFA
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes ａnd efficiently amplifies a specific target gene＇s cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+ ａnd no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; ａnd (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis ａnd gel electrophoresis.
GeneQuery™ Human Astrocyte Cell Biology qPCR Array Plate Layout* (8 controls in Bold ａnd Italic)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | ANGPT1 | CDH2 | DVL3 | EREG | FOXO1 | IL1B | KIT | MMP9 | PDGFRA | SELE | TGFB1 | β-actin |
| B | ANGPT2 | CDH5 | EDN1 | FGF1 | FZD4 | IL6 | KLF2 | NOS3 | PDGFRB | SEMA3F | TGFB2 | GAPDH |
| C | BAIAP3 | CNTN1 | EDNRA | FGF2 | HAND2 | ITGA5 | KLK3 | NOTCH1 | PECAM1 | SERPINE1 | THBS1 | LDHA |
| D | BMP1 | COL4A3 | EFNB2 | FIGF | HEY2 | ITGAV | LAMA5 | NOTCH4 | PF4 | SERPINF1 | TNFAIP1 | NONO |
| E | CCL2 | CX3CL1 | EGLN1 | FLT1 | HHEX | ITGB1 | LGALS9 | NPPB | PLAU | SMAD2 | TYMP | PPIH |
| F | CCL5 | CXCR4 | ENG | FN1 | HIF1A | ITGB3 | MAGI1 | NRP1 | PODXL | SMAD3 | VCAM1 | GDC |
| G | CD36 | DICER1 | EPHA2 | FOS | ICAM1 | JAG1 | MEOX2 | NRP2 | PTGS2 | SPHK1 | VEGFA | PPC |
| H | CD82 | DLL4 | ERBB2 | FOXC2 | ID1 | KDR | MMP2 | PARD3 | RUNX2 | TEK | VEGFC | NTC |
* gene selection may be updated based on new research ａnd development
产品使用说明:仅供科研研究使用
原创作者：上海雅吉生物科技有限公司
