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人抗中性粒细胞核周抗体(pANCA)ELISA试剂盒*今品化学新闻48T/96T
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人抗中性粒细胞核周抗体(pANCA)elisa试剂盒*今品化学新闻操作步骤:
用于检测未知抗原的双抗体夹心法
1. 包被:用0.05MPH9.牰碳酸盐包被缓冲液将抗体稀释至蛋白质含量为1~10μg/ml。在每个聚苯乙烯板的反应孔中加0.1ml,4℃ 过夜。次日,弃去孔内溶液,用洗涤缓冲液洗3次,每次3分钟。(简称洗涤,下同)。
2. 加样:加一定稀释的待检样品0.1ml于上述已包被之反应孔中,置37℃ 孵育1小时。然后洗涤。(同时做空白孔,阴性对照孔及阳性对照孔)。
3. 加酶标抗体:于各反应孔中,加入新鲜稀释的酶标抗体(经滴定后的稀释度)0.1ml。37℃ 孵育0.5~1小时,洗涤。
4. 加底物液显色:于各反应孔中加入临时配制的TMB底物溶液0.1ml,37℃ 10~30分钟。
5. 终止反应:于各反应孔中加入2M硫酸0.05ml。
人抗中性粒细胞核周抗体(pANCA)ELISA试剂盒*今品化学新闻温馨说明:
1仔细阅读说明书
2确定试剂盒在有效期内
3按说明书确定所有试剂齐全(数量、体积)
4标本制备要规范,每份标本体积要按2-3个复孔以上的量制备(贮存),尽量分装做备份。短期内无法实验者,注意低温保存
5准备好所有实验额外所需物品,如试管、吸头、移液器、离心机等
6根据检测标本数量确定所需试剂的量
7按说明书将所用试剂平衡至室温,配制所需试剂。
了解更多人抗中性粒细胞核周抗体(pANCA)ELISA试剂盒*今品化学新闻相关产品:
| 100ug/100ul | CD1B Polyclonal Antibody | P29016 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | T-cell surface glycoprotein CD1b (CD antigen CD1b) | |||
| 100ug/100ul | Integrin αX Polyclonal Antibody | P20702 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Integrin alpha-X (CD11 antigen-like family member C) (Leu M5) (Leukocyte adhesion glycoprotein p150,95 alpha chain) (Leukocyte adhesion receptor p150,95) (CD antigen CD11c) | |||
| 100ug/100ul | CD16 Polyclonal Antibody | P08637/O75015 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Low affinity immunoglobulin gamma Fc region receptor III-A/B (CD16a/b antigen) (Fc-gamma RIII-alpha/beta) (Fc-gamma RIII) (Fc-gamma RIIIa) (FcRIII) (FcRIIIa) (FcR-10) (IgG Fc receptor III-2) (CD antigen CD16a) | |||
| 100ug/100ul | CD64 Polyclonal Antibody | 45 | P12314 | H | WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000 | Rabbit | High affinity immunoglobulin gamma Fc receptor I (IgG Fc receptor I) (Fc-gamma RI) (FcRI) (Fc-gamma RIA) (FcgammaRIa) (CD antigen CD64) | ||
| 100ug/100ul | CD109 Polyclonal Antibody | Q6YHK3 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | CD109 antigen (150 kDa TGF-beta-1-binding protein) (C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7) (Platelet-specific Gov antigen) (p180) (r150) (CD antigen CD109) | |||
| 100ug/100ul | CD303 Polyclonal Antibody | Q8WTT0 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | C-type lectin domain family 4 member C (Blood dendritic cell antigen 2) (BDCA-2) (C-type lectin superfamily member 7) (Dendritic lectin) (CD antigen CD303) | |||
| 100ug/100ul | CD272 Polyclonal Antibody | Q7Z6A9 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | B- and T-lymphocyte attenuator (B- and T-lymphocyte-associated protein) (CD antigen CD272) | |||
| 100ug/100ul | CD151 Polyclonal Antibody | P48509 | H, M, R | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | CD151 antigen (GP27) (Membrane glycoprotein SFA-1) (Platelet-endothelial tetraspan antigen 3) (PETA-3) (Tetraspanin-24) (Tspan-24) (CD antigen CD151) | |||
| 100ug/100ul | CD152 Polyclonal Antibody | P16410 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Cytotoxic T-lymphocyte protein 4 (Cytotoxic T-lymphocyte-associated antigen 4) (CTLA-4) (CD antigen CD152) | |||
| 100ug/100ul | PD-L2 Polyclonal Antibody | 30 | Q9BQ51 | H | WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000 | Rabbit | Programmed cell death 1 ligand 2 (PD-1 ligand 2) (PD-L2) (PDCD1 ligand 2) (Programmed death ligand 2) (Butyrophilin B7-DC) (B7-DC) (CD antigen CD273) | ||
| 100ug/100ul | CD300e Polyclonal Antibody | Q496F6 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | CMRF35-like molecule 2 (CLM-2) (CD300 antigen-like family member E) (CMRF35-A5) (Immune receptor expressed on myeloid cells 2) (IREM-2) (Polymeric immunoglobulin receptor 2) (PIgR-2) (PIgR2) (Poly-Ig receptor 2) (CD antigen CD300e) | |||
| 100ug/100ul | CD158k Polyclonal Antibody | P43630 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Killer cell immunoglobulin-like receptor 3DL2 (CD158 antigen-like family member K) (MHC class I NK cell receptor) (Natural killer-associated transcript 4) (NKAT-4) (p70 natural killer cell receptor clone CL-5) (p70 NK receptor CL-5) (CD antigen CD158k) | |||
| 100ug/100ul | CD158f1/2 Polyclonal Antibody | Q8N109/Q8NHK3 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Killer cell immunoglobulin-like receptor 2DL5A/B (CD antigen CD158f1/2) | |||
| 100ug/100ul | APRIL Polyclonal Antibody | O75888 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Tumor necrosis factor ligand superfamily member 13 (A proliferation-inducing ligand) (APRIL) (TNF- and APOL-related leukocyte expressed ligand 2) (TALL-2) (TNF-related death ligand 1) (TRDL-1) (CD antigen CD256) | |||
| 100ug/100ul | DDR2 Polyclonal Antibody | Q16832 | H, M | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Discoidin domain-containing receptor 2 (Discoidin domain receptor 2) (EC 2.7.10.1) (CD167 antigen-like family member B) (Discoidin domain-containing receptor tyrosine kinase 2) (Neurotrophic tyrosine kinase, receptor-related 3) (Receptor protein-tyrosine kinase TKT) (Tyrosine-protein kinase TYRO10) (CD antigen CD167b) | |||
| 100ug/100ul | CD218b Polyclonal Antibody | O95256 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Interleukin-18 receptor accessory protein (IL-18 receptor accessory protein) (IL-18RAcP) (Accessory protein-like) (AcPL) (CD218 antigen-like family member B) (CDw218b) (IL-1R accessory protein-like) (IL-1RAcPL) (Interleukin-1 receptor 7) (IL-1R⁻⁷) (IL-1R7) (Interleukin-18 receptor accessory protein-like) (Interleukin-18 receptor beta) (IL-18R-beta) (IL-18Rbeta) (CD antigen CD218b) | |||
| 100ug/100ul | CKR-1 Polyclonal Antibody | P32246 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | C-C chemokine receptor type 1 (C-C CKR-1) (CC-CKR-1) (CCR-1) (CCR1) (HM145) (LD78 receptor) (Macrophage inflammatory protein 1-alpha receptor) (MIP-1alpha-R) (RANTES-R) (CD antigen CD191) | |||
| 100ug/100ul | CD297 Polyclonal Antibody | Q93070 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Ecto-ADP-ribosyltransferase 4 (EC 2.4.2.31) (ADP-ribosyltransferase C2 and C3 toxin-like 4) (ARTC4) (Dombrock blood group carrier molecule) (Mono(ADP-ribosyl)transferase 4) (NAD(P)(+)--arginine ADP-ribosyltransferase 4) (CD antigen CD297) | |||
| 100ug/100ul | CD327 Polyclonal Antibody | O43699 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Sialic acid-binding Ig-like lectin 6 (Siglec-6) (CD33 antigen-like 1) (CDw327) (Obesity-binding protein 1) (OB-BP1) (CD antigen CD327) | |||
| 100ug/100ul | CD231 Polyclonal Antibody | P41732 | H, M, R | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Tetraspanin⁻⁷ (Tspan⁻⁷) (Cell surface glycoprotein A15) (Membrane component chromosome X surface marker 1) (T-cell acute lymphoblastic leukemia-associated antigen 1) (TALLA-1) (Transmembrane 4 superfamily member 2) (CD antigen CD231) | |||
| 100ug/100ul | CD204 Polyclonal Antibody | P21757 | H, M, R | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Macrophage scavenger receptor types I and II (Macrophage acetylated LDL receptor I and II) (Scavenger receptor class A member 1) (CD antigen CD204) | |||
| 100ug/100ul | BTN3A1/2/3 Polyclonal Antibody | O00481/P78410/O00478 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Butyrophilin subfamily 3 member A1/2/3 (CD antigen CD277) | |||
| 100ug/100ul | CD300b Polyclonal Antibody | A8K4G0 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | CMRF35-like molecule 7 (CLM⁻⁷) (CD300 antigen-like family member B) (CMRF35-A2) (Immune receptor expressed on myeloid cells 3) (IREM-3) (Leukocyte mono-Ig-like receptor 5) (Triggering receptor expressed on myeloid cells 5) (TREM-5) (CD antigen CD300b) | |||
| 100ug/100ul | CD299 Polyclonal Antibody | 60 | Q9H2X3 | H, M, R | WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000 | Rabbit | C-type lectin domain family 4 member M (CD209 antigen-like protein 1) (DC-SIGN-related protein) (DC-SIGNR) (Dendritic cell-specific ICAM-3-grabbing non-integrin 2) (DC-SIGN2) (Liver/lymph node-specific ICAM-3-grabbing non-integrin) (L-SIGN) (CD antigen CD299) | ||
| 100ug/100ul | CD236 Polyclonal Antibody | P04921 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Glycophorin-C (Glycoconnectin) (Glycophorin-D) (GPD) (Glycoprotein beta) (PAS-2') (Sialoglycoprotein D) (CD antigen CD236) | |||
| 100ug/100ul | CCL14 Polyclonal Antibody | Q16627 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | C-C motif chemokine 14 (Chemokine CC-1/CC-3) (HCC-1/HCC-3) (HCC-1(1⁻⁷4)) (NCC-2) (Small-inducible cytokine A14) [Cleaved into: HCC-1(3⁻⁷4); HCC-1(4⁻⁷4); HCC-1(9⁻⁷4)] | |||
| 100ug/100ul | PDGF-C Polyclonal Antibody | Q9NRA1 | H, M, R | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Platelet-derived growth factor C (PDGF-C) (Fallotein) (Spinal cord-derived growth factor) (SCDGF) (VEGF-E) [Cleaved into: Platelet-derived growth factor C, latent form (PDGFC latent form); Platelet-derived growth factor C, receptor-binding form (PDGFC receptor-binding form)] | |||
| 100ug/100ul | Gremlin-2 Polyclonal Antibody | 19 | Q9H772 | H, M | WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000 | Rabbit | Gremlin-2 (Cysteine knot superfamily 1, BMP antagonist 2) (DAN domain family member 3) (Protein related to DAN and cerberus) | ||
| 100ug/100ul | IL-19 Polyclonal Antibody | 20 | Q9UHD0 | H, R | WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000 | Rabbit | Interleukin-19 (IL-19) (Melanoma differentiation-associated protein-like protein) (NG.1) | ||
| 100ug/100ul | IL-1F7 Polyclonal Antibody | Q9NZH6 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Interleukin-37 (FIL1 zeta) (IL-1X) (Interleukin-1 family member 7) (IL-1F7) (Interleukin-1 homolog 4) (IL-1H) (IL-1H4) (Interleukin-1 zeta) (IL-1 zeta) (Interleukin-1-related protein) (IL-1RP1) (Interleukin-23) (IL-37) | |||
| 100ug/100ul | MIP-3α Polyclonal Antibody | P78556 | H, M | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | C-C motif chemokine 20 (Beta-chemokine exodus-1) (CC chemokine LARC) (Liver and activation-regulated chemokine) (Macrophage inflammatory protein 3 alpha) (MIP-3-alpha) (Small-inducible cytokine A20) [Cleaved into: CCL20(1-67); CCL20(1-64); CCL20(2⁻⁷0)] | |||
| 100ug/100ul | BMP-9 Polyclonal Antibody | Q9UK05 | H, M | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Growth/differentiation factor 2 (GDF-2) (Bone morphogenetic protein 9) (BMP-9) | |||
| 100ug/100ul | NNT-1 Polyclonal Antibody | Q9UBD9 | H, M, R | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Cardiotrophin-like cytokine factor 1 (B-cell-stimulating factor 3) (BSF-3) (Novel neurotrophin-1) (NNT-1) | |||
| 100ug/100ul | Thrombospondin 4 Polyclonal Antibody | P35443 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Thrombospondin-4 | |||
| 100ug/100ul | Eotaxin-2 Polyclonal Antibody | O00175 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | C-C motif chemokine 24 (CK-beta-6) (Eosinophil chemotactic protein 2) (Eotaxin-2) (Myeloid progenitor inhibitory factor 2) (MPIF-2) (Small-inducible cytokine A24) | |||
| 100ug/100ul | MIP-5 Polyclonal Antibody | Q16663 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | C-C motif chemokine 15 (Chemokine CC-2) (HCC-2) (Leukotactin-1) (LKN-1) (MIP-1 delta) (Macrophage inflammatory protein 5) (MIP-5) (Mrp-2b) (NCC-3) (Small-inducible cytokine A15) [Cleaved into: CCL15(22-92); CCL15(25-92); CCL15(29-92)] | |||
| 100ug/100ul | ENA⁻⁷8 Polyclonal Antibody | P42830 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | C-X-C motif chemokine 5 (ENA⁻⁷8(1⁻⁷8)) (Epithelial-derived neutrophil-activating protein 78) (Neutrophil-activating peptide ENA⁻⁷8) (Small-inducible cytokine B5) [Cleaved into: ENA⁻⁷8(8⁻⁷8); ENA⁻⁷8(9⁻⁷8)] | |||
| 100ug/100ul | IL-20 Polyclonal Antibody | Q9NYY1 | H, M, R | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Interleukin-20 (IL-20) (Cytokine Zcyto10) | |||
| 100ug/100ul | PBP Polyclonal Antibody | P02775 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Platelet basic protein (PBP) (C-X-C motif chemokine 7) (Leukocyte-derived growth factor) (LDGF) (Macrophage-derived growth factor) (MDGF) (Small-inducible cytokine B7) [Cleaved into: Connective tissue-activating peptide III (CTAP-III) (LA-PF4) (Low-affinity platelet factor IV); TC-2; Connective tissue-activating peptide III(1-81) (CTAP-III(1-81)); Beta-thromboglobulin (Beta-TG); Neutrophil-activating peptide 2(74) (NAP-2(74)); Neutrophil-activating peptide 2(73) (NAP-2(73)); Neutrophil-activating peptide 2 (NAP-2); TC-1; Neutrophil-activating peptide 2(1-66) (NAP-2(1-66)); Neutrophil-activating peptide 2(1-63) (NAP-2(1-63))] | |||
| 100ug/100ul | IFN-β Polyclonal Antibody | P01574 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Interferon beta (IFN-beta) (Fibroblast interferon) | |||
| 100ug/100ul | Artn Polyclonal Antibody | Q5T4W7 | H | IHC-p 1:50-200, ELISA 1:10000-20000 | Rabbit | Artemin (Enovin) (Neublastin) |
热门标签:抗中性粒细胞核周抗体试剂盒,pANCA,elisa,抗中性粒细胞核周抗体,elisa













