当前位置: 阿仪网 > 生物试剂/抗体/细胞 > 试剂盒 > 人ELISA试剂盒 > 今品化学技术(上海)有限公司 > 产品展示 > ELISA检测试剂盒 > 人ELISA检测试剂盒 > 人细胞角蛋白18(CK-18)ELISA试剂盒*新闻快讯48T/96T
人细胞角蛋白18(CK-18)ELISA试剂盒*新闻快讯48T/96T
企业档案
会员类型:标准版
已获得阿仪网信誉 等级评定
(0 -40)基础信誉积累,可浏览访问
(41-90)良好信誉积累,可接洽商谈
(91+ )优质信誉积累,可持续信赖
阿仪网标准版会员:9年
最后认证时间:
注册号: 【已认证】
法人代表: 【已认证】
参观次数:3251677
详细内容
ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫学活性,酶标记的抗原或抗体既保留其免疫学活性,又保留酶的活性。在测定时,受检标本(测定其中的抗体或抗原)与固相载体表面的抗原或抗体起反应。用洗涤的方法使固相载体上形成的抗原抗体复合物与液体中的其他物质分开。再加入酶标记的抗原或抗体,也通过反应而结合在固相载体上。此时固相上的酶量与标本中受检物质的量呈一定的比例。加入酶反应的底物后,底物被酶催化成为有色产物,产物的量与标本中受检物质的量直接相关,故可根据呈色的深浅进行定性或定量分析。
二.实验材料与试剂配制:
1.仪器与材料:酶标仪(使用前预热30分钟),微量加液器、吸头、蒸馏水或去离子水,滤纸。
2. 洗液的配制:按1:30的比例配制洗液备用。
人细胞角蛋白18(CK-18)ELISA试剂盒*新闻快讯样品收集、处理及保存
1).细胞培养上清:
适用于检测体外培养的细胞分泌性成份。用无菌管收集细胞上清液,以1000×g离心15分钟,收集上清。
2)细胞:
用PBS反复洗涤细胞3次,调整细胞浓度达到104-106/ml 左右,通过反复冻融,使细胞破坏并放出细胞内成份,或者细胞超声粉碎,离心取上清液检测。
3)血清:
在室温下,血液自然凝固,以1000×g离心15分钟,取上清待测。
4)血浆:
应根据标本的要求选择EDTA 或柠檬酸钠作为抗凝剂,混合后静置10-20 分钟后,以1000×g离心15分钟,收集上清。
5)体液:
包括胸腹水、脑脊液,分泌物等。使用不含热原和内毒素的离心管收集,以1000×g离心15分钟,收集上清。
6.)组织标本:
切取组织标本,称取重量0.5mg,加入500ul 的PBS,用手工或匀浆器,或超声破碎仪将标本匀浆,以2000-3000 rmp离心20 分钟,收集上清进行检测。
样品中不能含有NaN3,因NaN3 抑制辣根过氧化物酶的(HRP)活性。
人细胞角蛋白18(CK-18)ELISA试剂盒*新闻快讯保存:
如果样品不能立即检测,应将其分装,⁻⁷0 ℃保存,避免反复冷冻。保存过程中如出现沉淀,应再次离心。血液标本尽可能的不要使用溶血或高血脂血。如果血清中含大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
| Dulbecco's Modification of Eagles Medium (DMEM) with 4.5 g/L glucose and sodium pyruvate without L-glutamine,500mL | DMEM培养基 (高糖, 不含丙酮酸钠) | Media Standard |
| Minimum Essential Medium (MEM) Alpha Medium with Earle's salts, ribonucleosides, deoxyribonucleosides, and L-glutamine | AMEM培养基 (含谷氨酰胺, 丙酮酸钠) | Media Standard |
| F-12K Nutrient Mixture (Kaighn's Mod.) with L-glutamine | F-12K培养基,含L-谷氨酰胺 | Media Standard |
| RPMI 1640 with L-glutamine,500mL | RPMI1640培养基 (含谷氨酰胺) | Media Standard |
| RPMI 1640 with L-glutamine and 25 mM HEPES | RPMI1640培养基 (含谷氨酰胺,HEPES) | Media Standard |
| Leibovitz's L-15 with L-glutamine | Leibovitz's L-15 培养基 | Media Standard |
| Medium 199 (Mod.) with Earle's salts and L-glutamine | M199培养基 (含谷氨酰胺) | Media Standard |
| 500 mL Ham's F-10 Medium with L-glutamine | 500ml Ham's F-10 培养基,含谷氨酰胺 | Media Standard |
| Ham's F-12 Medium with L-glutamine | Ham's F12 培养基 (含谷氨酰胺) | Media Standard |
| Dulbecco's Modification of Eagle's Medium/Ham's F-12 50/50 Mix | DMEM/F12培养基 (含谷氨酰胺, HEPES) | Media Standard |
| Corning® 500 mL Graces Insect Basal Medium (Vaughn Mod.) [+] L-glutamine, [-] insect hemolymph | Grace 昆虫培养基 | Specialty Products |
| DMEM High Glu, w/o Gln, w/Pyr | DMEM培养基 (高糖, 含丙酮酸钠,不含谷氨酰胺) | Media Standard |
| 500 mL DMEM (Dulbeco's Modification of Eagle's Medium) with 4.5 g/L glucose, without L-glutamine, sodium pyruvate | DMEM培养基 (高糖, 不含谷氨酰胺,丙酮酸钠) | Media Standard |
| RPMI 1640 1X, w/o Gln | RPMI1640培养基 (不含谷氨酰胺) | Media Standard |
| RPMI 1640 w/o Gln w/25mM Hepes | RPMI1640培养基 (含HEPES,不含谷氨酰胺) | Media Standard |
| DMEM/F12 50:50 Mix w/o Gln | DMEM/F12培养基 (不含谷氨酰胺) | Media Standard |
| 500 mL CMRL 1066 without L-glutamine | CMRL 1066(含谷氨酰胺) | Media Standard |
| 500 mL DMEM (Dulbecco's Modification of Eagle's Medium)/Ham's F-12 50/50 Mix with L-glutamine, without phenol red | DMEM/F12培养基,不含酚红 | Media Standard |
| Hank's Balanced Salt Solution, 1X without calcium and magnesium | HBSS平衡盐溶液 (含钙, 镁) | Media Standard |
| 500 mL HBSS (Hank's Balanced Salt Solution), 1x without calcium and magnesium | HBSS平衡盐溶液 (不含钙, 镁) | Media Standard |
| Hank's Balanced Salt Solution, 1X without calcium, magnesium and phenol red | HBSS平衡盐溶液 (不含钙, 镁, 酚红) | Media Standard |
| Dulbecco's Phosphate-Buffered Saline, 1X without calcium and magnesium | DPBS缓冲液 (不含钙, 镁) | Media Standard |
| Phosphate-Buffered Saline, 1X without calcium and magnesium | PBS缓冲液 (不含钙, 镁) | Media Standard |
| Sodium Pyruvate, Liquid 100 mM Solution with 8.5 g/L NaCI | 丙酮酸钠溶液 (100x) | Media Standard |
| Corning® 100 mL L-Glutamine, 200 mM solution, 100x [+] 8.5 g/L NaCl | L-谷氨酰胺 (200 mM 溶液) | Media Standard |
| Corning® 100 mL glutagro™, 200 mM Solution, 100x [+] 8.5 g/L NaCI | L-谷氨酰胺替代物 | Media Standard |
| MEM Nonessential Amino Acids | MEM非必需氨基酸溶液 (100x) | Media Standard |
| 100 mL 45% glucose solution | 45%葡萄糖溶液 | Media Standard |
| Corning® 100 mL Trypsin EDTA 1x, 0.05% Trypsin/0.53 mM EDTA in HBSS [+] sodium bicarbonate, [-]calcium and magnesium, Porcine Parvovirus Tested. | 0.05%胰酶/0.53 mM EDTA | Media Standard |
| Corning® 100 mL Trypsin EDTA 1x, 0.25% Trypsin/2.21 mM EDTA in HBSS [-] sodium bicarbonate, calcium, magnesium, Porcine Parvovirus Tested | 0.25% 胰酶/2.21 mM EDTA | Media Standard |
| Corning® 1 L Cell Culture Grade Water Tested to USP Sterile Water for Injection Specifications | 无菌注射级用水-细胞培养等级 | Media Standard |
| Corning® 500 mL Cell Culture Grade Water Tested to USP Sterile Water for Injection Specifications | 无菌注射级用水-细胞培养等级 | Media Standard |
| Corning® 100 mL Cellstripper™, Liquid | Cellstripper细胞消化液 | Media Standard |
| Corning® 100 mL HEPES, Liquid 1M Solution (238.3 mg/mL) | HEPES 缓冲盐 (1M) | Media Standard |
| Corning® 100 mL LSM (Lymphocyte Separation Medium) | 淋巴细胞分离培养基 | Specialty Products |
| Corning® 500 mL LSM (Lymphocyte Separation Medium) | 淋巴细胞分离培养基 | Specialty Products |
| Corning® 10 mL ITS (Insulin-Transferrin-Selenium), 100x | 胰岛素-转铁蛋白-硒 | Media Standard |
| Corning® 500 mL Fetal Bovine Serum, Australia Sourced | 澳洲胎牛血清 | Serum |
| ADCF CHO-2 medium | ADCF CHO-2 培养基 | Specialty Products |
| transfectaGRO Reduced Serum Medium | transfectaGRO 减血清/转染用培养基 | Specialty Products |
| Corning® 450 mL/50 mL stemgro® hMSC Medium and Supplement | Stemgro 基础培养基 | Specialty Products |
| 500 mL Hepatocyte Maintenance Medium | 肝细胞维持培养液 | Specialty Products |
| Corning® 1 L 10X PBS (Phosphate Buffered Saline), pH 7.4 ± 0.1, Liquid [-] calcium and magnesium, RNase-/DNase- and proteasefree | 10* PBS缓冲液 1L, pH 7.4不含钙镁 | Media Standard |
热门标签:人细胞角蛋白18,CK-18,ELISA,人细胞角蛋白18试剂盒













