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小鼠血小板生成素(TPO)ELISA试剂盒*今品化学新闻48T/96T
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详细内容
1. 抗原固定(包埋):将对应疫苗用0.05M的碳酸钠(Na2CO3)溶液稀释至2 微克/毫升, 在96孔ELISA薄板上每孔加100µL, 于37度2小时或4度冰箱过夜。第二天
2. 封闭(BLOCKING):用PBSt冲洗薄板三次,每次必须把孔中液体拍掉. 再在每孔加1%脱指奶粉或BSA或0.1%吐温(用PBSt稀释)100µL,37度下放置30分钟.
3. 用PBSt冲洗薄板3次,拍干孔中液体。
4. 滴度测试,每个样本用8孔测试。
(1)如样本为血清,则每孔加上100µL含5µg/mL抑制疫苗(用0.1%BSA的PBSt液稀释),抑制疫苗为偶联物和要检测抗体的疫苗的偶联物一样的另一种疫苗。
(2)如样本为已纯化的抗体,则不用加抑制疫苗。
5. (1)血清样本用含5µg/mL抑制疫苗溶液(用0.1%BSA的PBSt液稀释)稀释400倍.在第一孔加100µL(总量为200µL/孔),混匀后移液100µL到第二孔,同样混匀后从第二孔移液100µL到第三孔•••••如此直至第7孔,留第八孔为空白对照。这样从1⁻⁷孔的最终样本稀释度分别为:1:800,1:1600,1:3200, 1:6400, 1: 12800, 1: 25600, 1: 51200。(2)抗体样本则稀释1000倍,混匀后移液50µL到第二孔.....至第七孔,留第八孔为空白对照。
6. 稀释完后在37度反应60分钟.
7. 吸掉每孔中的液体,用PBSt分别清洗4次,每次拍干孔内液体.
8. 上二抗:将抗兔IgG HRP用PBSt稀释,稀释倍数(1:5000-1:20000不等)由其浓度决定,每孔加100µL,37度反应30分钟后重复清洗步奏4次。
9. 拍干后每孔加入100µL的过氧化物酶(HRP)底物TMB生色. 37度大概15-30分钟每孔加100µL2M硫酸终止反应,然后在酶联测试仪于波长450纳米读数.
10. 结果判断、记录:计算OD为50%时,血清(或抗体)的最大稀释度,以最大稀释度为样品的滴度,记录滴度,并将实验结果报告相关人员。
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| Bacitracin zinc | 1405-89-6 |
| Ammonium Glycyrrhizinate (AMGZ) | 1407-03-0 |
| Levosimendan | 141505-33-1 |
| Sulfamethizole (Proklar) | 144-82-1 |
| Diphenhydramine HCl (Benadryl) | 147-24-0 |
| Thiamphenicol (Thiophenicol) | 15318-45-3 |
| Dimesna | 16208-51-8 |
| Ezetimibe (Zetia) | 163222-33-1 |
| Clevudine (Levovir) | 163252-36-6 |
| Sitafloxacin hydrate | 163253-35-8,163253-37-0(H2O),127254-12-0(freebase),346607-37-2(HCl) |
| Levamisole HCl(Ergamisol) | 16595-80-5 |
| Conivaptan HCl (Vaprisol) | 168626-94-6 |
| Posaconazole | 171228-49-2,177571-33-4,1198769-38-8(H2O) |
| Allopurinol Sodium (Aloprim) | 17795-21-0 |
| Cabazitaxel (Jevtana) | 183133-96-2,890654-44-1 |
| Telithromycin (Ketek) | 191114-48-4 |
| Prazosin HCl | 19237-84-4,19216-56-9(freebase) |
| Tiopronin (Thiola) | 1953-02-2 |
| Resistomycin | 20004-62-0,1393-09-5 |
| Deferasirox (Exjade) | 201530-41-8 |
| Flucytosine (Ancobon) | 2022-85⁻⁷ |
| Oxibendazole | 20559-55-1 |
| BIBR 953(Dabigatran etexilate, Pradaxa) | 211914-51-1 |
| Tempol (4-Hydroxy-TEMPO) | 2226-96-2 |
| Cathepsin Inhibitor 1 | 225120-65-0 |
| Aspartame | 22839-47-0 |
| Peramivir | 229614-55-5,330600-85-6,1041434-82-5(3H2O) |
| Articaine HCl | 23964-57-0,23964-58-1(freebase) |
| Econazole nitrate (Spectazole) | 24169-02-6 |
| Mycophenolic (Mycophenolate) | 24280-93-1 |
| Geniposidic acid | 27741-01-1,103530-48-9(monosodiumsalt) |
| Bumetanide | 28395-03-1,28434⁻⁷4-4(sodiumsalt) |
| Ciclopirox (Penlac) | 29342-05-0 |
| Ticarcillin sodium | 29457-07-6,34787-01-4(freebase) |
| Teniposide (Vumon) | 29767-20-2 |
| Methoxsalen (Oxsoralen) | 298-81⁻⁷,12692-94-3 |
| Gossypol | 303-45⁻⁷,40112-23-0,732279-01-5 |
| Hydralazine HCl | 304-20-1 |
| Calcitriol (Rocaltrol) | 32222-06-3 |
| Tobramycin | 32986-56-4 |
| Fenoprofen calcium | 34597-40-5 |
| Amoxicillin sodium (Amox) | 34642⁻⁷7-8 |
| Buflomedil HCl | 35543-24-9 |
| Fluocinonide(Vanos) | 356-12⁻⁷ |
| Ecdysone | 3604-87-3 |
| Prostaglandin E2 (Cervidil) | 363-24-6 |
| Doripenem Hydrate | 364622-82-2,148016-81-3(freebase) |
| Ribavirin (Copegus) | 36791-04-5 |
| Chlorhexidine HCl | 3697-42-5,55-56-1(freebase) |
| Azalomycin-B | 37318-06-2,11003-23-9,106387-86-4 |
| Betamethasone (Celestone) | 378-44-9,956901-32-9(XHCl) |
| Streptomycin sulfate | 3810⁻⁷4-0 |
| Sulindac (Clinoril) | 38194-50-2,63804-15-9(sodiumsalt) |
| Nalidixic acid (NegGram) | 389-08-2 |
| Suprofen (Profenal) | 40828-46-4 |
| Flumequine | 42835-25-6,143984-63-8,42835-68⁻⁷(sodiumsalt) |
| Fenbendazole (Panacur) | 43210-67-9 |
| Azathioprine (Azasan, Imuran) | 446-86-6 |
| DL-Carnitine HCl | 461-05-2 |
| Lactulose (Enulose) | 4618-18-2,149252-91-5(3H2O) |
| Nobiletin (Hexamethoxyflavone) | 478-01-3 |
| Rhein(Monorhein) | 478-43-3,202053-60-9(monosodiumsalt) |
| Vecuronium Bromide | 50700⁻⁷2-6,86029-43-8(freebase),74531⁻⁷6-3(Maleicacid) |
| Acipimox | 51037-30-0,76958-97-9(sodiumsalt) |
| Flurbiprofen (Ansaid) | 5104-49-4 |
| L-NAME HCl | 51298-62-5,50903-99-6(freebase) |
| Pizotifen malate | 5189-11⁻⁷,15574-96-6(freebase) |
| Hesperidin | 520-26-3 |
| Rheochrysidin (Physcione) | 521-61-9,140670-52-6 |
| Pranoprofen | 52549-17-4 |
| Kinetin (6-Furfuryladenine) | 525⁻⁷9-1 |
| Penicillamine (Cuprimine) | 52-67-5 |
| Haloperidol (Haldol) | 52-86-8 |
| Salinomycin (Procoxacin) | 53003-10-4 |
| Prednisone(Adasone) | 53-03-2 |
| Mitotane (Lysodren) | 53-19-0,102976-59-0 |
| Ethionamide | 536-33-4 |
| Oxfendazole | 53716-50-0 |
| Indomethacin (Indocid, Indocin) | 53-86-1 |
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