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小鼠维生素B12(VB12)ELISA试剂盒*今品化学新闻48T/96T
价格：￥998
品牌名称：$brandModel.Title型号：48T/96T原产地：中国大陆 更新时间：2026/10/22 11:06:36
产品摘要：小鼠维生素B12(VB12)ELISA试剂盒*今品化学新闻为今品化学技术（上海）有限公司主打产品，质量好,发货及时,灵敏度高，特异性强，重复性好，其他动物类试剂盒种属一应俱全,指标数千种。公司配有专业实验室可寄样免费代检测支持现场观摩,提供售前售中售后全方位服务，欢迎来电询价或在线咨询索取产品说明书~
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详细内容
小鼠维生素B12(VB12)elisa试剂盒*今品化学新闻操作步骤
1. 标准品的稀释与加样：在酶标包被板上设标准品孔 10 孔，在第一、第二孔中分别加标
准品 100µl，然后在第一、第二孔中加标准品稀释液50µl，混匀；然后从第一孔、第二
孔中各取 100µl分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液 50µl，
混匀；然后在第三孔和第四孔中先各取 50µl弃掉，再各取 50µl分别加到第五、第六孔
中，再在第五、第六孔中分别加标准品稀释液 50ul，混匀；混匀后从第五、第六孔中各
取 50µl 分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液 50µl，混
匀后从第七、第八孔中分别取 50µl 加到第九、第十孔中，再在第九第十孔分别加标准
品稀释液 50µl，混匀后从第九第十孔中各取 50µl弃掉。（稀释后各孔加样量都为 50µl，
浓度分别为 150 pg/mL，100 pg/mL ，50 pg/mL，25pg/mL，12.5 pg/mL）。
2. 加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、待测样
品孔。在酶标包被板上待测样品孔中先加样品稀释液 40µl，然后再加待测样品 10µl（样
品最终稀释度为 5 倍）。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混
匀。
3. 温育：用封板膜封板后置37℃温育 30 分钟。
4. 配液：将 30（48T的 20倍）倍浓缩洗涤液用蒸馏水30（48T的 20倍）倍稀释后备用。
5. 洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置 30 秒后弃去，如此
重复 5次，拍干。
6. 加酶：每孔加入酶标试剂50µl，空白孔除外。
7. 温育：操作同 3。
8. 洗涤：操作同 5。
9. 显色：每孔先加入显色剂 A50µl，再加入显色剂 B50µl，轻轻震荡混匀，37℃避光显色
15 分钟.
10. 终止：每孔加终止液 50µl，终止反应（此时蓝色立转黄色）。
11. 测定：以空白空调零，450nm波长依序测量各孔的吸光度（OD 值）。 测定应在加终止
液后 15 分钟以内进行。
小鼠维生素B12(VB12)ELISA试剂盒*今品化学新闻注意事项：
新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-80℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。
安全性：
1． 避免直接接触终止液和底物。如不小心接触到这些液体，请尽快用水冲洗。
2． 实验中不要吃喝、抽烟或使用化妆品。
3． 不要用嘴接触试剂盒里的任何成份。
4 Elisa试剂盒请远离儿童
自备物品：
1 、 37 ℃ 恒温箱2 、 标准规格酶标仪3 、 精密移液器及一次性吸头4 、 蒸馏水
5 、 一次性试管6 、 吸水纸
小鼠维生素B12(VB12)ELISA试剂盒*今品化学新闻相关产品发布：
| 100μg/100μl | ASK1 (Phospho-Ser83) Antibody | Q99683 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | SEK1/MKK4 (Phospho-Ser80) Antibody | P45985 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~100 | |
| 100μg/100μl | SEK1/MKK4 (Phospho-Thr261) Antibody | P45985 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
| 100μg/100μl | PLCγ2(Phospho-Tyr753) Antibody | P16885 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | AMPK1/AMPK2 (Phospho-Ser485/Ser491) Antibody | Q13131 P54646 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | PKCθ(Phospho-Ser695) Antibody | Q04759 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
| 100μg/100μl | PKCβ(Phospho-Thr641) Antibody | P05771 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50-1:100 | |
| 100μg/100μl | α-Synuclein (Phospho-Ser129) Antibody | P37840 | Human, Mouse, Rat | WB:1:500~1000 | |
| 100μg/100μl | NMDAR2B (phospho-Tyr1474) antibody | Q13224 | Human, Mouse, Rat | IF:1:100~1:200 | |
| 100μg/100μl | DNA PKcs (Phospho-Thr2609) antibody | P78527 | Human | WB: 1:500~1:1000 | |
| 100μg/100μl | NFκB-p65 (Phospho-Thr505) Antibody | Q04206 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
| 100μg/100μl | PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody | Q13153//Q13177//O75914 | Human, Mouse, Rat | IHC: 1:50-1:100 | |
| 100μg/100μl | HSP27 (Phospho-Ser15) Antibody | P04792 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200 | |
| 100μg/100μl | STAT1(Phospho-Ser727) Antibody | P42224 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | IκB-α (Phospho-Tyr42) Antibody | P25963 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
| 100μg/100μl | MEK1 (Phospho-Ser221) Antibody | Q02750 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | Zap⁻⁷0 (Phospho-Tyr493) Antibody | P43403 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
| 100μg/100μl | Zap⁻⁷0 (Phospho-Tyr319) Antibody | P43403 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | VASP (Phospho-Ser238) Antibody | P50552 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | FKHRL1 (Phospho-Ser253) Antibody | O43524 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | eNOS (Phospho-Ser1176) Antibody | P29474 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
| 100μg/100μl | PAK1(Phospho-Thr212) Antibody | Q13153 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50-1:100 | |
| 100μg/100μl | Src (Phospho-Tyr529) Antibody | P12931 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | IκB-α (Phospho-Ser32/Ser36) Antibody | P25963 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
| 100μg/100μl | JAK2 (Phospho-Tyr1007) Antibody | O60674 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
| 100μg/100μl | JAK1(Phospho-Tyr1022) Antibody | P23458 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | TYK2 (Phospho-Tyr1054) Antibody | P29597 | Human, Mouse | WB: 1:500~1:1000 | |
| 100μg/100μl | Chk2 (Phospho-Ser516) Antibody | O96017 | Human | WB: 1:500~1:1000 | |
| 100μg/100μl | MKK6 (Phospho-Ser207) Antibody | P52564 | Human, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200 | |
| 100μg/100μl | MKK3 (Phospho-Ser189) Antibody | P46734 | Human, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200 | |
| 100μg/100μl | Lck (Phospho-Tyr393) Antibody | P06239 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | IRS-1 (Phospho-Ser312) Antibody | P35568 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | Vav (Phospho-Tyr174) Antibody | P15498 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | Chk1 (Phospho-Ser280) Antibody | O14757 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000 | |
| 100μg/100μl | cofilin (Phospho-Ser3) Antibody | P23528 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | cdc25A (Phospho-Ser75) Antibody | P30304 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | AFX (Phospho-Ser197) Antibody | P98177 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | FKHR (Phospho-Ser319) Antibody | Q12778 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | CDC2 (Phospho-Thr161) Antibody | P06493 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | CDK2 (Phospho-Thr160) Antibody | P24941 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000 | |
| 100μg/100μl | Rb (Phospho-Ser780) Antibody | P06400 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | Rb (Phospho-Ser807) Antibody | P06400 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | Rb (Phospho-Ser795) Antibody | P06400 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | IKK α (Phospho-Thr23) Antibody | O15111 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | HDAC8 (Phospho-Ser39) Antibody | Q9BY41 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
| 100μg/100μl | LIMK2 (Phospho-Thr505) Antibody | P53671 | Human, Mouse, Rat | WB: 1:500~1000 IHC: 1:50~1:100 | |
| 100μg/100μl | LIMK1 (Phospho-Thr508) Antibody | P53667 | Human, Mouse, Rat | WB: 1:500~1000 IHC: 1:50~1:100 | |
| 100μg/100μl | c-Abl (Phospho-Tyr412) Antibody | P00519 | Human, Mouse | IHC: 1:50~1:100 | |
| 100μg/100μl | Akt2 (Phospho-Ser474) Antibody | P31751 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | FAK (Phospho-Tyr925) Antibody | Q05397 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | ATM (Phospho-Ser1981) Antibody | Q13315 | Human, Mouse | IHC: 1:50~1:100 | |
| 100μg/100μl | Chk1 (Phospho-Ser345) Antibody | O14757 | Human, Mouse, Rat | IHC: 1:50~1:100 | |
| 100μg/100μl | Androgen Receptor (Phospho-Ser650) Antibody | P10275 | Human | IHC: 1:50~1:100 | |
| 100μg/100μl | Androgen Receptor (Phospho-Ser213) Antibody | P10275 | Human | WB:1:500~1000 | |
| 100μg/100μl | cdc25C (Phospho-Ser216) Antibody | P30307 | Human | IHC: 1:50~1:100 IF: 1:100-200 | |
| 100μg/100μl | BRCA1 (Phospho-Ser1524) Antibody | P38398 | Human | IHC: 1:50~1:100 | |
| 100μg/100μl | β-Catenin (Phospho-Thr41/Ser45) Antibody | P35222 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | FKHR (Phospho-Ser256) Antibody | Q12778 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | Myosin Light Chain 2 (Phospho-Ser18) Antibody | P78527 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | p90RSK (Phospho-Ser352) Antibody | P18653 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | Tau (Phospho-Ser404) Antibody | P10636 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | Tau (Phospho-Ser262) Antibody | P10636 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | Tau(Phospho-Thr231) Antibody | P10636 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | Tau (Phospho-Ser214) Antibody | P10636 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | Tau (Phospho-Thr205) Antibody | P10636 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | Tau (Phospho-Thr181) Antibody | P10636 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC:1:50-100 | |
| 100μg/100μl | Tau (Phospho-Ser235) Antibody | P10636 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | p90RSK(Phospho-Thr348) Antibody | Q15418 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50-1:100 | |
| 100μg/100μl | NMDAR1 (Phospho-Ser896) Antibody | Q05586 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | PLCγ1 (Phospho-Tyr783) Antibody | P19174 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | Tau (Phospho-Ser396) Antibody | P10636 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | Tau(Phospho-Ser356) Antibody | P10636 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | p53 (Phospho-Ser315) Antibody | P04637 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | p53 (Phospho-Ser37) Antibody | P04637 | Human | WB: 1:500~1:1000 | |
| 100μg/100μl | p53 (Phospho-Ser33) Antibody | P04637 | Human | WB: 1:500~1:1000 | |
| 100μg/100μl | PKD/PKCμ (Phospho-Ser910) Antibody | Q15139 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | p53 (Phospho-Thr18) Antibody | P04637 | Human | WB: 1:500~1:1000 | |
| 100μg/100μl | p53 (Phospho-Ser6) Antibody | P04637 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | Src(Phospho-Tyr418) Antibody | P12931 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | Caveolin-1 (Phospho-Tyr14) Antibody | Q03135 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | Paxillin (Phospho-Tyr118) Antibody | P49023 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50-1:100 | |
| 100μg/100μl | IGF-1R (Phospho-Tyr1165/Tyr1166) Antibody | P08069 | Human, Mouse, Rat | WB: 1:500~1:1000 IF:1:100~1:200 | |
| 100μg/100μl | IGF-1R (Phospho-Tyr1161) Antibody | P08069 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200 | |
| 100μg/100μl | VEGFR2 (Phospho-Tyr1214) Antibody | P35968 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200 | |
| 100μg/100μl | VEGFR2 (Phospho-Tyr1175) Antibody | P35968 | Human, Mouse, Rat | IHC: 1:50~1:100 IF:1:100~1:200 | |
| 100μg/100μl | ICAM-1(Phospho-Tyr512) Antibody | P05362 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | G3BP-1 (Phospho-Ser232) Antibody | Q13283 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200 | |
| 100μg/100μl | EGFR (Phospho-Tyr1092) Antibody | P00533 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | EGFR (Phospho-Ser1070) Antibody | P00533 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 | |
| 100μg/100μl | HER2 (Phospho-Tyr1248) Antibody | P04626 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200 | |
| 100μg/100μl | MyoD (Phospho-Ser200) Antibody | P15172 | Human, Mouse, Rat | WB: 1:500~1:1000 | |
| 100μg/100μl | HER2 (Phospho-Tyr1221/Tyr1222) Antibody | P04626 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200 | |
| 100μg/100μl | HER2 (Phospho-Tyr877) Antibody | P04626 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200 | |
| 100μg/100μl | Progesterone Receptor (Phospho-Ser190) Antibody | P06401 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200 | |
| 100μg/100μl | Estrogen Receptor-α (Phospho-Ser167) Antibody | P03372 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200 | |
| 100μg/100μl | Estrogen Receptor-α (Phospho-Ser118) Antibody | P03372 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200 | |
| 100μg/100μl | Estrogen Receptor-α (Phospho-Ser106) Antibody | P03372 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200 | |
| 100μg/100μl | BAD (Phospho-Ser155) Antibody | Q92934 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 |
热门标签：小鼠维生素B12,VB12,,ELISA,小鼠维生素B12试剂盒
