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小鼠甲基化酶(Methylase)ELISA试剂盒*上海新闻48T/96T
价格：￥998
品牌名称：$brandModel.Title型号：48T/96T原产地：中国大陆 更新时间：2026/10/22 11:06:42
产品摘要：小鼠甲基化酶(Methylase)ELISA试剂盒*上海新闻为今品化学技术（上海）有限公司主打产品，质量好,发货及时,灵敏度高，特异性强，重复性好，其他动物类试剂盒种属一应俱全,指标数千种。公司配有专业实验室可寄样免费代检测支持现场观摩,提供售前售中售后全方位服务，欢迎来电询价或在线咨询索取产品说明书~
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详细内容
小鼠甲基化酶(Methylase)elisa试剂盒*上海新闻操作步骤：
用于检测未知抗原的双抗体夹心法
1. 包被：用0.05MPH9.牰碳酸盐包被缓冲液将抗体稀释至蛋白质含量为1～10μg／ml。在每个聚苯乙烯板的反应孔中加0.1ml，4℃ 过夜。次日，弃去孔内溶液，用洗涤缓冲液洗3次，每次3分钟。（简称洗涤，下同）。
2. 加样：加一定稀释的待检样品0.1ml于上述已包被之反应孔中，置37℃ 孵育1小时。然后洗涤。（同时做空白孔，阴性对照孔及阳性对照孔）。
3. 加酶标抗体：于各反应孔中，加入新鲜稀释的酶标抗体（经滴定后的稀释度）0.1ml。37℃ 孵育0.5～1小时，洗涤。
4. 加底物液显色：于各反应孔中加入临时配制的TMB底物溶液0.1ml，37℃ 10～30分钟。
5. 终止反应：于各反应孔中加入2M硫酸0.05ml。
小鼠甲基化酶(Methylase)ELISA试剂盒*上海新闻温馨说明：
1仔细阅读说明书
2确定试剂盒在有效期内
3按说明书确定所有试剂齐全（数量、体积）
4标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。短期内无法实验者，注意低温保存
5准备好所有实验额外所需物品，如试管、吸头、移液器、离心机等
6根据检测标本数量确定所需试剂的量
7按说明书将所用试剂平衡至室温，配制所需试剂。
了解更多小鼠甲基化酶(Methylase)ELISA试剂盒*上海新闻相关产品：
| BCL-2 (Phospho-Ser87) Antibody | P10415 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| Aurora Kinase (Ab-288) Antibody | O14965 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:5000 | |
| Aurora Kinase (Phospho-Thr288) Antibody | O14965 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| ATPase (Ab-16) Antibody | P05023 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| ATPase (Phospho-Ser16) Antibody | P05023 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| AS160 (Ab-642) Antibody | O60343 | Human, Mouse | IF: 1:100~1:500 ELISA: 1:1000 | |
| AS160 (Phospho-Thr642) Antibody | O60343 | Human, Mouse | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| Artemis Antibody | Q96SD1 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| Artemis (Phospho-Ser516) Antibody | Q96SD1 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| Arrestin 1 (Ab-412) Antibody | P49407 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| Arrestin 1 (Phospho-Ser412) Antibody | P49407 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| Akt1 S1 (Ab-246) Antibody | Q96B36 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| Akt1 S1 (Phospho-Thr246) Antibody | Q96B36 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| ADD1 Antibody | P35611 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| ADD1 (Phospho-Thr445) Antibody | P35611 | Human, Mouse, Rat | IHC: 1:50~1:100 ELISA: 1:1000 | |
| Ku80 (Ab⁻⁷14) Antibody | P13010 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| Ku80 (Phospho-Thr714) Antibody | P13010 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| Ku70 (Ab-5) Antibody | P12956 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| Ku70 (Phospho-Ser5) Antibody | P12956 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| KOR-1 (Ab-369) Antibody | P41145 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:5000 | |
| KOR-1 (Phospho-Ser369) Antibody | P41145 | Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
| IRS-1 (Ab-612) Antibody | P35568 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| IRS-1 (Phospho-Ser612) Antibody | P35568 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| IRS-1 Antibody | P35568 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:5000 | |
| IRS-1 (Phospho-Ser1101) Antibody | P35568 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| Integrin β1 (Ab⁻⁷88) Antibody | P05556 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
| Integrin β1 (Phospho-Thr788) Antibody | P05556 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| IL3R (Ab-593) Antibody | P26951 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| IL-3Rβ (Phospho-Tyr593) Antibody | P26951 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| IKK-γ (Ab-31) Antibody | Q9Y6K9 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| IKK-γ (Phospho-Ser31) Antibody | Q9Y6K9 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| IKK-β (Ab-188) Antibody | O14920 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| IKK-β (Phospho-Tyr188) Antibody | O14920 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| IKK-α/β (Ab-176/177) Antibody | O15111/O14920 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| IKK-α (Phospho-Ser176)/IKK-β (Phospho-Ser177) Antibody | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | ||
| IKK-α/β (Ab-180/181) Antibody | O15111/O14920 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| IKK-α/β (Phospho-Ser180/181) Antibody | O15111/O14920 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| IκB-β (Ab-19) Antibody | Q15653 | Human | WB: 1:500~1:1000 ELISA: 1:1000 | |
| IκB-β (Phospho-Thr19) Antibody | Q15653 | Human | WB: 1:500~1:1000 ELISA: 1:1000 | |
| HSL (Ab-552) Antibody | Q05469 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| HSL (Phospho-Ser552) Antibody | Q05469 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| HDAC5 (Ab-259) Antibody | Q9UQL6 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| HDAC5 (Phospho-Ser259) Antibody | Q9UQL6 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
| Histone H3 (Ab-28) Antibody | P68431 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| Histone H3 (Phospho-Ser28) Antibody | P68431 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
| Histone H3 (Ab-3) Antibody | P68431 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| Histone H3 (Phospho-Thr3) Antibody | P68431 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| GRF-1 (Ab-1105) Antibody | Q9NRY4 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| GRF-1 (Phospho-Tyr1105) Antibody | Q9NRY4 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| GR (Ab-226) Antibody | P04150 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| GR (Phospho-Ser226) Antibody | P04150 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| GlycogenSynthase(Ab-645)Antibody | P13807 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| Glycogen Synthase (Phospho-Ser645) Antibody | P13807 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| Fyn (Ab-530) Antibody | P06241 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
| Fyn (Phospho-Tyr530) Antibody | P06241 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| Fos (Ab-362) Antibody | P01100 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| Fos (Phospho-Ser362) Antibody | P01100 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| FANCD2 (Ab-222) Antibody | Q9BXW9 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| FANCD2 (Phospho-Ser222) Antibody | Q9BXW9 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| FAK (Ab-576) Antibody | Q05397 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| FAK (Phospho-Tyr576) Antibody | Q05397 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| FADD (Ab-194) Antibody | Q13158 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| FADD (Phospho-Ser194) Antibody | Q13158 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| E2F1 (Ab-433) Antibody | Q01094 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
| E2F1 (Phospho-Thr433) Antibody | Q01094 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:5000 |
热门标签：小鼠甲基化酶,Methylase,ELISA,小鼠甲基化酶试剂盒
