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小鼠补体片断5a(C5a)ELISA试剂盒*上海新闻48T/96T
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小鼠补体片断5a(C5a)elisa试剂盒*上海新闻测试步骤
(1)仔细切开覆盖在孔板上的箔片,从微孔板底部用手指轻轻顶出所用的孔板。注意不要损伤不使用的孔板上的条或孔,而且应将其立即用塑料袋密封放入2-8℃的冰箱保存,以防止琼脂变干;
(2)揭开覆盖在孔板上的箔片,每孔加入80mL牛奶样品。并分别加入80mL的阴性和阳性质控,加入样品时应避免交叉污染;
(3)用密封胶将孔板密封,置于65℃恒温箱或水浴箱中孵育直到阴性质控转化成浅黄绿色(参照每批质量检测报告,大约需要1小时45分钟)。
注:如果是采用水浴进行孵育的话,请注意避免水进入微孔。样品孵育时间不能超过质检报告中说明的时间,否则检测灵敏度会下降。
小鼠补体片断5a(C5a)ELISA试剂盒*上海新闻结果分析:
孵育完毕后取出微孔板,如果采用水浴法,用吸水纸吸干外壁的水分。在微孔后面放置一张白纸并观察孔内的颜色变化,从微孔壁侧面所能观察的颜色比从顶部或者底部所能观察到的更准确。有时候孵育时在微孔侧旁会出现气泡,可以在没有气泡的另一侧进行观察。对于一些颜色介于阴性和阳性质控的样品,需要进行更进一步的分析和重测。
试剂的准备孔板(条或孔)和阳性和阴性质控临用前取出,应在室温下放置至少半小时。不使用的微孔板应置于2-8°C保存,剩余的阳性和阴性质控应放回原处冰冻保存。
小鼠补体片断5a(C5a)ELISA试剂盒*上海新闻相关试剂盒展示:
| Ataxin 1 (Phospho-Ser776) Antibody | P54253 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
| A-RAF (Ab-301/302) Antibody | P10398 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| A-RAF (Phospho-Tyr302) Antibody | P10398 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| DRD1 Antibody | P21728 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 84%, Positives = 84%);Rat (Identities = 84%, Positives = 84%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| Arachidonate 5 Lipoxygenase (Ab-271) Antibody | P09917 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| Arachidonate 5 Lipoxygenase (Phospho-Ser271) Antibody | P09917 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| Aquaporin 2 (Ab-256) Antibody | P41181 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| Aquaporin 2 (Phospho-Ser256) Antibody | P41181 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:40000 | |
| Androgen Receptor (Ab-94) Antibody | P10275 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 88%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
| Androgen Receptor (Phospho-Ser94) Antibody | P10275 | Human, Mouse | IF: 1:100~1:500 ELISA: 1:5000 | |
| AMPK β1 (Ab-181) Antibody | Q9Y478 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
| AMPK β1 (Phospho-Ser181) Antibody | Q9Y478 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| AhR (Ab-36) Antibody | P35869 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| AhR (Phospho-Ser36) Antibody | P35869/A9YTQ3 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| Adrenergic Receptor B2 (Phospho-Ser355+Ser356) Antibody | P07550 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| ADAM 17 Antibody | P78536 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)Rat (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:1000 | |
| ADAM 17 (Phospho-Thr735) Antibody | P78536 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:20000 | |
| Actin-pan (α/β/γ) (Ab-53/55) Antibody | P60709/P63261 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| Actin-pan (α/γ) (Phospho-Tyr55/53) Antibody | P60709/P63261 | Human, Mouse | IHC: 1:50~1:100 ELISA: 1:5000 | |
| PFKFB2 (Ab-483) Antibody | O60825 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| PFKFB2 (Phospho-Ser483) Antibody | O60825 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
| 53BP1 (Ab-6) Antibody | Q12888 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
| 53BP1 (Phospho-Ser6) Antibody | Q12888 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| CYSLTR2 Antibody | Q9NS75 | Human | WB: 1:500~1:1000 ELISA: 1:20000 | |
| 14-3-3 ζ/δ (Ab-232) Antibody | P63104 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| 14-3-3 ζ/δ (Phospho-Thr232) Antibody | P63104 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| 14-3-3 θ/τ (Ab-232) Antibody | P27348 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
| 14-3-3 θ/τ (Phospho-Ser232) Antibody | P27348 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| YAP (Ab-127) Antibody | P46937 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
| YAP (Phospho-Ser127) Antibody | P46937 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
| CYSLTR1 Antibody | Q9Y271 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
| TLK1 Antibody | Q9Y4F6 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:5000 | |
| TIE2 Antibody | Q02763 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:5000 | |
| TIE2 (Phospho-Tyr1102) Antibody | Q02763 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:40000 | |
| CRHR2 Antibody | Q13324 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
| SHIP1 (Ab-1020) Antibody | Q92835 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:5000 | |
| SHIP1 (Phospho-Tyr1021) Antibody | Q92835 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:40000 | |
| SGK (Ab⁻⁷8) Antibody | O00141 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| SGK (Phospho-Ser78) Antibody | O00141 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| CRHR1 Antibody | P34998 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
| Ras-GRF1 (Ab-916) Antibody | Q13972 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 88%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| Ras-GRF1 (Phospho-Ser916) Antibody | Q13972 | Mouse, Rat | IHC: 1:50~1:100 ELISA: 1:20000 | |
| PP1-α (Ab-320) Antibody | P62136 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| PP1-α (Phospho-Thr320) Antibody | P62136 | Human, Mouse, Rat | IHC: 1:50~1:100 ELISA: 1:10000 | |
| PLD1 Antibody | Q13393 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| PLD1 (Phospho-Ser561) Antibody | Q13393 | Human, Mouse, Rat | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| PLC β3 Antibody | Q01970 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 88%, Positives = 100%);Rat (Identities = 88%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| PLC β3 (Phospho-Ser537) Antibody | Q01970 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| PKD1/PKC μ (Ab-910) Antibody | Q15139 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:5000 | |
| PKD1/PKC μ (Phospho-Ser910) Antibody | Q15139 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| PKD1/2/3/PKC μ (Ab⁻⁷44/748) Antibody | Q15139 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
| PKD1/2/3/PKC μ (Phospho-Ser738+Ser742) Antibody | Q15139 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| PKC θ (Ab-538) Antibody | Q04759 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| PKC θ (Phospho-Thr538) Antibody | Q04759 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| PKC δ (Phospho-Tyr313) Antibody | Q05655 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| PKC δ (Ab-505) Antibody | Q05655 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| PKC δ (Phospho-Thr505) Antibody | Q05655 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| PKC α (Ab-638) Antibody | P17252 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| PKC α (Phospho-Thr638) Antibody | P17252 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| Pin1 (Ab-1619) Antibody | Q13526 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| Pin1 (Phospho-Ser16) Antibody | Q13526 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| Pim-1 (Ab-309) Antibody | P06803 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| Pim-1 (Phospho-Tyr309) Antibody | P06803 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:40000 | |
| PEA-15 (Ab-104) Antibody | Q15121 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| PEA-15 (Phospho-Ser104) Antibody | Q15121 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| PBK/TOPK (Ab-9) Antibody | Q96KB5 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:000 | |
| PBK/TOPK (Phospho-Thr9) Antibody | Q96KB5 | Human | WB: 1:500~1:1000 ELISA: 1:40000 | |
| CLPT1 Antibody | O96005 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:10000 | |
| PAK4/5/6 (Ab-474) Antibody | O96013 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:5000 | |
| PAK2 (Ab-20) Antibody | Q13177 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| PAK2 (Phospho-Ser20) Antibody | Q13177 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
| p63 (Phospho-Ser455) Antibody | Q9H3D4 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| CCKBR Antibody | P32239 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:20000 | |
| p53 (Phospho-Thr81) Antibody | P04637 | Human | IHC: 1:50~1:100 ELISA: 1:20000 | |
| p40 phox Antibody | Q15080 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 88%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
| p40 phox (Phospho-Thr154) Antibody | Q15080 | Human, Mouse | IHC: 1:50~1:100 ELISA: 1:1000 | |
| p130 Cas (Phospho-Tyr249) Antibody | P56945 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| p130 Cas Antibody | P56945 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
| p130 Cas (Phospho-Tyr165) Antibody | P56945 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| NPM (Ab-199) Antibody | P06748 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
| NPM (Phospho-Thr199) Antibody | P06748 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 IHC: 1:50~1:100 ELISA: 1:60000 | |
| NMDAR1 (Ab-896) Antibody | Q05586 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| NMDAR1 (Phospho-Ser896) Antibody | Q05586 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
| NMDAR1 (Ab-890) Antibody | Q05586 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| NMDAR1 (Phospho-Ser890) Antibody | Q05586 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
| MRLC2 (Ab-18) Antibody | Q9CQL8 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| Myosin regulatory light chain 2 (Phospho-Ser18) Antibody | Q9CQL8 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| mTOR (Ab-2481) Antibody | P42345 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| mTOR (Phospho-Ser2481) Antibody | P42345 | Human, Mouse, Rat | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| Mst1/2 (Ab-183) Antibody | Q13043 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| Mst1/2 (Phospho-Thr183) Antibody | Q13043 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
| MSK1 (Ab-581) Antibody | O75582 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
| MSK1 (Phospho-Thr581) Antibody | O75582 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000 | |
| MSK1 (Ab-360) Antibody | O75582 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| MSK1 (Phospho-Ser360) Antibody | O75582 | Human, Mouse | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 |
热门标签:小鼠补体片断5a,C5a,,ELISA,小鼠补体片断5a试剂盒













