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小鼠抗内皮细胞抗体(AECA)ELISA试剂盒*上海新闻48T/96T
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品牌名称：$brandModel.Title型号：48T/96T原产地：中国大陆 更新时间：2026/10/22 11:06:42
产品摘要：小鼠抗内皮细胞抗体(AECA)ELISA试剂盒*上海新闻为今品化学技术（上海）有限公司主打产品，质量好,发货及时,灵敏度高，特异性强，重复性好，其他动物类试剂盒种属一应俱全,指标数千种。公司配有专业实验室可寄样免费代检测支持现场观摩,提供售前售中售后全方位服务，欢迎来电询价或在线咨询索取产品说明书~
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详细内容
【小鼠抗内皮细胞抗体(AECA)elisa试剂盒*上海新闻操作步骤】
1、准备：从冰箱取出试剂盒，室温复温平衡30分钟。
2、配液：用蒸馏水将20倍浓缩洗涤液稀释成原倍的洗涤液。
3、加标准品和待测样本：取足够数量的酶标包被板，固定于框架上，分别设置标准品孔、待测样本孔和空白对照孔，记录各孔位置，在标准品孔中加入标准品50μL；待测样本孔中先加入待测样本10μL，再加样本稀释液40μL（即样本稀释5倍）；空白对照孔不加。
4、温育：37℃水浴锅或恒温箱温育30min。
5、洗板：弃去液体，吸水纸上拍干，每孔加满洗涤液，静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板4次（也可用洗板机按说明书操作洗板）。
6、加酶标工作液：每孔加入酶标工作液50μL，空白对照孔不加。
7、温育：重复4的操作。
8、洗板：重复5的操作。
9、显色：每孔先加入显色剂A 液50μL，再加入显色剂B液 50μL，37℃避光显色15min。
10、终止：取出酶标板，每孔加终止液50μL，终止反应（颜色由蓝色立转黄色）。
11、测定：以空白孔调零，在终止后15分钟内，用450nm波长测量各孔的吸光值（OD值）。
12、计算：根据标准品的浓度及对应的OD值，计算出标准曲线的直线回归方程，再根据样本的OD值，在回归方程上计算出对应的样品浓度，也可以使用各种应用软件来计算。最终浓度为实际测定浓度乘以稀释倍数。
【小鼠抗内皮细胞抗体(AECA)ELISA试剂盒*上海新闻样本要求】
1、样本不能含叠氮钠（NaN3），因为叠氮钠（NaN3）是辣根过氧化物酶（HRP）的抑制剂。
2、标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能立即试验，可将标本放于-20℃保存，但应避免反复冻融。
3、样本应充分离心，不得有溶血及颗粒。
公司客户遍布华中华东华西华北华南,各大地区,售前售中售后全方位贴心服务，全国免费快递送货上门,需了解试剂盒详情,请点击本页右上方“QQ交谈”向本公司客服索取产品说明书。
今品化学技术（上海）有限公司一直秉承以用户需求为核心，在专注上海本地市场开拓的同时，为全国各地区科研单位提供实验高质量产品，优质、用心的服务赢得了众多同仁的信赖和好评，逐渐树立起公司良好品牌。公司不仅仅提供专业的科研产品，同时还建立了完善的售后服务体系，为您实验进行中遇到的问题和困难提供指导帮助。我司坚信，通过我们不断的努力和追求，一定能够实现与各大科研单位互利共赢！
小鼠抗内皮细胞抗体(AECA)ELISA试剂盒*上海新闻相关产品发布：
| Calcium Sensing Receptor (Ab-888) Antibody | P41180 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| Calcium Sensing Receptor (Phospho-Thr888) Antibody | P41180 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000 | |
| C/EBP-ε (Ab⁻⁷4) Antibody | Q15744 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| C/EBP-ε (Phospho-Thr74) Antibody | Q15744 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| C/EBP-α (Ab-222) Antibody | P49715 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
| C/EBP-α (Phospho-Thr226) Antibody | P49715 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| B-Myb (Ab-577/581) Antibody | P10244 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
| B-Myb (Phospho-Ser577/581) Antibody | P10244 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000 | |
| BAD (Ab-134) Antibody | Q92934 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| BAD (Phospho-Ser134) Antibody | Q92934 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| BAD (Ab-91/128) Antibody | Q92934 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
| BAD (Phospho-Ser91/128) Antibody | Q92934 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| CD234 Antibody | Q16570 | Human (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:40000 | |
| ATF2 (Ab-480) Antibody | P15336 | Human (Identities = 100%, Positives = 100%);Rat (Identities = 88%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| ATF2 (Phospho-Ser480) Antibody | P15336 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
| APC (Ab-2054) Antibody | P25054 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| APC (Phospho-Ser2054) Antibody | P25054 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
| Akt (Ab-326) Antibody | P31749 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| Akt (Phospho-Tyr326) Antibody | P31749 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:20000 | |
| TNNI3 (Ab-43) Antibody | P19429 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| TNNI3 (Phospho-Ser43) Antibody | P19429 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
| TIP60 (Ab-86) Antibody | Q92993 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| TIP60 (Phospho-Ser86) Antibody | Q92993 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:40000 | |
| RBL2 (Ab-952) Antibody | Q08999 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:1000 | |
| RBL1 (Ab-369) Antibody | P28749 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:1000 | |
| RBL1 (Phospho-Thr369) Antibody | P28749 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:5000 | |
| Retinoblastoma Antibody | P06400 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| Retinoblastoma (Phospho-Thr821) Antibody | P06400 | Human, Mouse, Rat | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
| Retinoblastoma (Ab-811) Antibody | P06400 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
| Retinoblastoma (Phospho-Ser811) Antibody | P06400 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
| PTP1B (Ab-50) Antibody | P18031 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| PTP1B (Phospho-Ser50) Antibody | P18031 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
| PKD2 (Ab-876) Antibody | Q9BZL6 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| PKD2 (Phospho-Ser876) Antibody | Q9BZL6 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:60000 | |
| PKD1/PKC μ (Ab-463) Antibody | Q15139 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| PKD1/PKC μ (Phospho-Tyr463) Antibody | Q15139 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
| PKD1/PKC μ (Ab-205) Antibody | Q15139 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| PKD1/PKC μ (Phospho-Ser205) Antibody | Q15139 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 | |
| PKC ζ (Ab-560) Antibody | Q05513 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| PKC ζ (Phospho-Thr560) Antibody | Q05513 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| PKC θ (Ab-695) Antibody | Q04759 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:5000 | |
| PKC θ (Phospho-Ser695) Antibody | Q04759 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| PKC-pan (Ab-Thr497) Antibody | P17252/P05771/Q05655/Q02156/ P05129/P24723/Q04759/Q05513 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| PKC-pan (Phospho-Thr497) Antibody | P17252/P05771/Q05655/Q02156/ P05129/P24723/Q04759/Q05513 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
| PKC ε (Ab⁻⁷29) Antibody | P17252/P05771/Q05655/Q02156/ P05129/P24723/Q04759/Q05513 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| PKC ε (Phospho-Ser729) Antibody | Q02156 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| PKC δ (Ab-52) Antibody | Q05655 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:3000 IF: 1:100~1:500 ELISA: 1:10000 | |
| PKC δ (Phospho-Tyr52) Antibody | Q05655 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
| PKC α (Ab-657) Antibody | P17252 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:5000 | |
| PKC α (Phospho-Tyr657) Antibody | P17252 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
| DRD4 Antibody | P21917 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000 | |
| p38 MAPK (Ab-179/181) Antibody | Q16539 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| p38MAPK (Phospho-Thr179+Tyr181) Antibody | Q16539 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:5000 | |
| p38 MAPK (Ab-322) Antibody | Q16539 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| p38 MAPK (Phospho-Tyr322) Antibody | Q16539 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| HDAC1 (Ab-421) Antibody | Q13547 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| HDAC1 (Phospho-Ser421) Antibody | Q13547 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:5000 | |
| Histone H3.3 (Ab-31) Antibody | P84243 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| Histone H3.3 (Phospho-Ser31) Antibody | P84243 | Human, Mouse, Rat | IF: 1:100~1:500 ELISA: 1:20000 | |
| Histone H2B (Ab-14) Antibody | P33778 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:40000 | |
| GAB1 (Ab-659) Antibody | Q13480 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| GAB1 (Phospho-Tyr659) Antibody | Q13480 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| ETK (Ab-566) Antibody | P51813 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| ETK (Phospho-Tyr566) Antibody | P51813 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| Chk2 (Ab-383) Antibody | O96017 | Human, Mouse, Rat | IHC: 1:50~1:100 ELISA: 1:5000 | |
| Chk2 (Phospho-Thr383) Antibody | O96017 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
| Breast Tumor Kinase Antibody | Q13882 | Human (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:1000 | |
| Breast Tumor Kinase (Phospho-Tyr447) Antibody | Q13882 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
| B-Raf (Ab-601) Antibody | P15056 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| B-RAF (Phospho-Ser602) Antibody | P15056 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| B-RAF (Ab-598) Antibody | P15056 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:5000 |
热门标签：小鼠抗内皮细胞抗体,AECA,ELISA,小鼠抗内皮细胞抗体试剂盒
