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小鼠中性粒细胞明胶酶相关脂质运载蛋白(NGAL)ELISA试剂盒*上海新闻48T/96T
价格：￥1050
品牌名称：$brandModel.Title型号：48T/96T原产地：中国大陆 更新时间：2026/10/22 11:06:45
产品摘要：小鼠中性粒细胞明胶酶相关脂质运载蛋白(NGAL)ELISA试剂盒*上海新闻为今品化学技术（上海）有限公司主打产品，质量好,发货及时,灵敏度高，特异性强，重复性好，其他动物类试剂盒种属一应俱全,指标数千种。公司配有专业实验室可寄样免费代检测支持现场观摩,提供售前售中售后全方位服务，欢迎来电询价或在线咨询索取产品说明书~
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详细内容
小鼠中性粒细胞明胶酶相关脂质运载蛋白(NGAL)elisa试剂盒*上海新闻操作步骤
1. 标准品的稀释与加样：在酶标包被板上设标准品孔 10 孔，在第一、第二孔中分别加标
准品 100µl，然后在第一、第二孔中加标准品稀释液50µl，混匀；然后从第一孔、第二
孔中各取 100µl分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液 50µl，
混匀；然后在第三孔和第四孔中先各取 50µl弃掉，再各取 50µl分别加到第五、第六孔
中，再在第五、第六孔中分别加标准品稀释液 50ul，混匀；混匀后从第五、第六孔中各
取 50µl 分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液 50µl，混
匀后从第七、第八孔中分别取 50µl 加到第九、第十孔中，再在第九第十孔分别加标准
品稀释液 50µl，混匀后从第九第十孔中各取 50µl弃掉。（稀释后各孔加样量都为 50µl，
浓度分别为 150 pg/mL，100 pg/mL ，50 pg/mL，25pg/mL，12.5 pg/mL）。
2. 加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、待测样
品孔。在酶标包被板上待测样品孔中先加样品稀释液 40µl，然后再加待测样品 10µl（样
品最终稀释度为 5 倍）。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混
匀。
3. 温育：用封板膜封板后置37℃温育 30 分钟。
4. 配液：将 30（48T的 20倍）倍浓缩洗涤液用蒸馏水30（48T的 20倍）倍稀释后备用。
5. 洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置 30 秒后弃去，如此
重复 5次，拍干。
6. 加酶：每孔加入酶标试剂50µl，空白孔除外。
7. 温育：操作同 3。
8. 洗涤：操作同 5。
9. 显色：每孔先加入显色剂 A50µl，再加入显色剂 B50µl，轻轻震荡混匀，37℃避光显色
15 分钟.
10. 终止：每孔加终止液 50µl，终止反应（此时蓝色立转黄色）。
11. 测定：以空白空调零，450nm波长依序测量各孔的吸光度（OD 值）。 测定应在加终止
液后 15 分钟以内进行。
小鼠中性粒细胞明胶酶相关脂质运载蛋白(NGAL)ELISA试剂盒*上海新闻注意事项：
新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-80℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。
安全性：
1． 避免直接接触终止液和底物。如不小心接触到这些液体，请尽快用水冲洗。
2． 实验中不要吃喝、抽烟或使用化妆品。
3． 不要用嘴接触试剂盒里的任何成份。
4 Elisa试剂盒请远离儿童
小鼠中性粒细胞明胶酶相关脂质运载蛋白(NGAL)ELISA试剂盒*上海新闻自备物品：
1 、 37 ℃ 恒温箱2 、 标准规格酶标仪3 、 精密移液器及一次性吸头4 、 蒸馏水
5 、 一次性试管6 、 吸水纸
| 100μg | CBL (Ab⁻⁷74) Antibody | P22681 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | CBL (Phospho-Tyr774) Antibody | P22681 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | Calnexin (Ab-583) Antibody | P27824 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | Calnexin (Phospho-Ser583) Antibody | P27824 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | Caldesmon (Ab⁻⁷89) Antibody | Q05682 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | Caldesmon (Phospho-Ser789) Antibody | Q05682 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | BID (Ab⁻⁷8) Antibody | P55957 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| 100μg | BID (Phospho-Ser78) Antibody | P55957 | Human, Mouse | IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg | BCL-2 (Phospho-Ser87) Antibody | P10415 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | Aurora Kinase (Ab-288) Antibody | O14965 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:5000 | |
| 100μg | Aurora Kinase (Phospho-Thr288) Antibody | O14965 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | ATPase (Ab-16) Antibody | P05023 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | ATPase (Phospho-Ser16) Antibody | P05023 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | AS160 (Ab-642) Antibody | O60343 | Human, Mouse | IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | AS160 (Phospho-Thr642) Antibody | O60343 | Human, Mouse | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | Artemis Antibody | Q96SD1 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | Artemis (Phospho-Ser516) Antibody | Q96SD1 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | Arrestin 1 (Ab-412) Antibody | P49407 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | Arrestin 1 (Phospho-Ser412) Antibody | P49407 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | Akt1 S1 (Ab-246) Antibody | Q96B36 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | Akt1 S1 (Phospho-Thr246) Antibody | Q96B36 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| 100μg | ADD1 Antibody | P35611 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | ADD1 (Phospho-Thr445) Antibody | P35611 | Human, Mouse, Rat | IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg/100μl | Ku80 (Ab⁻⁷14) Antibody | P13010 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg/100μl | Ku80 (Phospho-Thr714) Antibody | P13010 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | Ku70 (Ab-5) Antibody | P12956 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg | Ku70 (Phospho-Ser5) Antibody | P12956 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | KOR-1 (Ab-369) Antibody | P41145 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg | KOR-1 (Phospho-Ser369) Antibody | P41145 | Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
| 100μg | IRS-1 (Ab-612) Antibody | P35568 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | IRS-1 (Phospho-Ser612) Antibody | P35568 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg | IRS-1 Antibody | P35568 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg/100μl | IRS-1 (Phospho-Ser1101) Antibody | P35568 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg | Integrin β1 (Ab⁻⁷88) Antibody | P05556 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
| 100μg | Integrin β1 (Phospho-Thr788) Antibody | P05556 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg/100μl | IL3R (Ab-593) Antibody | P26951 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg/100μl | IL-3Rβ (Phospho-Tyr593) Antibody | P26951 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | IKK-γ (Ab-31) Antibody | Q9Y6K9 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg/100μl | IKK-γ (Phospho-Ser31) Antibody | Q9Y6K9 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg/100μl | IKK-β (Ab-188) Antibody | O14920 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg/100μl | IKK-β (Phospho-Tyr188) Antibody | O14920 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | IKK-α/β (Ab-176/177) Antibody | O15111/O14920 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg/100μl | IKK-α (Phospho-Ser176)/IKK-β (Phospho-Ser177) Antibody | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | ||
| 100μg | IKK-α/β (Ab-180/181) Antibody | O15111/O14920 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg | IKK-α/β (Phospho-Ser180/181) Antibody | O15111/O14920 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| 100μg | IκB-β (Ab-19) Antibody | Q15653 | Human | WB: 1:500~1:1000 ELISA: 1:1000 | |
| 100μg | IκB-β (Phospho-Thr19) Antibody | Q15653 | Human | WB: 1:500~1:1000 ELISA: 1:1000 | |
| 100μg | HSL (Ab-552) Antibody | Q05469 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg | HSL (Phospho-Ser552) Antibody | Q05469 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg/100μl | HDAC5 (Ab-259) Antibody | Q9UQL6 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg/100μl | HDAC5 (Phospho-Ser259) Antibody | Q9UQL6 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
| 100μg | Histone H3 (Ab-28) Antibody | P68431 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg/100μl | Histone H3 (Phospho-Ser28) Antibody | P68431 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:1000 | |
| 100μg | Histone H3 (Ab-3) Antibody | P68431 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg/100μl | Histone H3 (Phospho-Thr3) Antibody | P68431 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | GRF-1 (Ab-1105) Antibody | Q9NRY4 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | GRF-1 (Phospho-Tyr1105) Antibody | Q9NRY4 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg/100μl | GR (Ab-226) Antibody | P04150 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| 100μg/100μl | GR (Phospho-Ser226) Antibody | P04150 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg/100μl | GlycogenSynthase(Ab-645)Antibody | P13807 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| 100μg/100μl | Glycogen Synthase (Phospho-Ser645) Antibody | P13807 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg | Fyn (Ab-530) Antibody | P06241 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
| 100μg/100μl | Fyn (Phospho-Tyr530) Antibody | P06241 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | Fos (Ab-362) Antibody | P01100 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | Fos (Phospho-Ser362) Antibody | P01100 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg/100μl | FANCD2 (Ab-222) Antibody | Q9BXW9 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| 100μg/100μl | FANCD2 (Phospho-Ser222) Antibody | Q9BXW9 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | FAK (Ab-576) Antibody | Q05397 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg/100μl | FAK (Phospho-Tyr576) Antibody | Q05397 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg/100μl | FADD (Ab-194) Antibody | Q13158 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg/100μl | FADD (Phospho-Ser194) Antibody | Q13158 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg/100μl | E2F1 (Ab-433) Antibody | Q01094 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
| 100μg/100μl | E2F1 (Phospho-Thr433) Antibody | Q01094 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:5000 | |
| 100μg/100μl | Dynamin-1 (Ab⁻⁷74) Antibody | Q05193 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 |
热门标签：小鼠中性粒细胞明胶酶相关脂质运载蛋白,NGAL,ELISA,小鼠中性粒细胞明胶酶相关脂质运载蛋白试剂盒
