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双特异性蛋白磷酸酶10抗体实验步骤
点击次数： 95发布时间：2021/10/25 14:48:01
- 上 传 者： 上海古朵生物科技有限公司
- 上传时间： 2021/10/25 14:48:01
- 文件大小： 18KB
- 文件类型： docx
- 所属分类： 应用方法
- 下载次数： 5次
- 查看次数： 95
资料简介： 英文名称 Anti-DUSP10/MKP5 保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 双特异性蛋白磷酸酶10抗体产品介绍 Mitogen-activated protein (MAP) kinases are a large class of proteins involved in signal transduction pathways that are activated by a range of stimuli ａnd mediate a number of physiological ａnd pathological changes in the cell. Dual specificity phosphatases (DSPs) are a subclass of the protein tyrosine phosphatase (PTP) gene superfamily, which are selective for dephosphorylating critical phosphothreonine ａnd phosphotyrosine residues within MAP kinases. DSP gene expression is induced by a host of growth factors and/or cellular stresses, thereby negatively regulating MAP kinase superfamily members including MAPK/ERK, SAPK/JNK ａnd p38. MKP-5 preferentially binds to p38, but also to SAPK/JNK. It is ubiquitously expressed ａnd localizes to both the cylasm ａnd the nucleus. MKP-5 has been implicated in cell proliferation ａnd apoptosis, tumor invasion ａnd immune responses. Function : Involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily.
中文名称 双特异性蛋白磷酸酶10抗体
别 名 Dual specificity phosphatase 10; Dual specificity phosphatase MKP 5; Dual specificity phosphatase MKP5; Dual specificity protein phosphatase 10; DUS10_HUMAN; DUSP 10; DUSP10; DUSP10 protein; MAP kinase phosphatase 5; Mitogen activated protein kinase phosphatase 5; Mitogen-activated protein kinase phosphatase 5; MKP 5; MKP-5; MKP5; Serine/threonine specific protein phosphatase.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
产品类型 一抗
研究域 细胞生物 信号转导 细胞凋亡 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 53kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human DUSP10/MKP5
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Subunit : Monomer. Interacts with MAPK14.
Subcellular Location : Cylasm. Nucleus.
Tissue Specificity : Detected in brain.
Similarity : Belongs to the protein-tyrosine phosphatase family.
Non-receptor class dual specificity subfamily.
Contains 1 rhodanese domain.
Contains 1 tyrosine-protein phosphatase domain.
Database links : UniProtKB/Swiss-Prot: Q9Y6W6.1
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