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DNA甲基转移酶3L抗体实验方法
点击次数： 48发布时间：2021/10/19 15:12:36
- 上 传 者： 上海古朵生物科技有限公司
- 上传时间： 2021/10/19 15:12:36
- 文件大小： 18KB
- 文件类型： docx
- 所属分类： 应用方法
- 下载次数： 4次
- 查看次数： 48
资料简介： 中文名称 DNA甲基转移酶3L抗体 DNA甲基转移酶3L抗体产品介绍 Function : Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A ａnd DNMT3B. Activates DNMT3A ａnd DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA ａnd AdoMet to the methyltransferases ａnd dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) ａnd induces de novo DNA methylation by recruitment or activation of DNMT3. Subunit : Homodimer. Heterotetramer composed of 1 DNMT3A homodimer ａnd 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with histone H3 (via N-terminus). Subcellular Location : Nucleus. 【存储要求】连续使用时4°C存储，保质期六个月；DNA甲基转移酶3L抗体期存储时建议分装为10ul以上小包装-20°C存储，并避免反复冻融，保质期一年。 实验的用途：
别 名 Cytosine 5 methyltransferase 3 like protein; DNA (cytosine 5 ) methyltransferase 3 like; DNA (cytosine-5)-methyltransferase 3-like; DNA Cytosine 5 Methyltransferase 3 Like Protein; DNA Methyltransferase 3 Like Protein; DNM3L_HUMAN; Dnmt 3L; Dnmt3l; Human cytosine 5 methyltransferase 3 like protein; MGC1090.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat
产品类型 一抗
研究域 细胞生物 表观遗传学
蛋白分子量 predicted molecular weight: 44kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Dnmt3L
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Methylation at the 5＇-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5＇-position of cytosines, thereby repressing expression of the target gene. Dnmt3L (DNA (cytosine-5)-methyltransferase 3-like) is a 387 amino acid protein that contains one ADD-type zinc finger ａnd is a member of the Dnmt family. Localized to the nucleus ａnd expressed at lows levels in thymus, testis ａnd ovary, Dnmt3L does not exhibit DNA methyltransferase activity, but is able to stimulate de novo methylation by Dnmt3 ａnd is thought to play a key role in the establishment of genomic imprints. Additionally, Dnmt3L interacts with histone deacetylase 1 (HDAC1) and, through this interaction, mediates transcriptional repression. Multiple isoforms of Dnmt3L exist due to alternative splicing events.
Tissue Specificity : Expressed at low levels in several tissues including testis, ovary, ａnd thymus.
Similarity : Belongs to the C5-methyltransferase family.
Contains 1 ADD-type zinc finger.
Database links : UniProtKB/Swiss-Prot: Q9UJW3.3
1）WB：Western Blotting 免疫印迹
2）IH：Immunohistochemistry 免疫组化
3）IH(P)：Immunohistochemistry Parraffin sections 免疫组化(石蜡)
4）IH(F)：Immunohistochemistry Frozen sections 免疫组化(冰冻)
5）IC：Immunocytochemistry 免疫细胞化学
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