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脱帽酶1A抗体纯化步骤
点击次数： 48发布时间：2021/10/11 15:24:53
- 上 传 者： 上海古朵生物科技有限公司
- 上传时间： 2021/10/11 15:24:53
- 文件大小： 18KB
- 文件类型： docx
- 所属分类： 应用方法
- 下载次数： 3次
- 查看次数： 48
资料简介： 英文名称 DCP1A Function: Subunit: Subcellular Location:
中文名称 脱帽酶1A抗体
别 名 DCP1 decapping enzyme homolog A; Dcp1a; DCP1A_HUMAN; mRNA decapping enzyme 1A; mRNA-decapping enzyme 1A; Smad4 interacting transcriptional co activator; Smad4-interacting transcriptional co-activator; Smad4-interacting transcriptional co-activator; SMAD4IP1; SMIF; Transcription factor SMIF.
研究域 细胞生物 信号转导 表观遗传学
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 脱帽酶1A抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 63kDa
细胞定位 细胞核 细胞浆
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human DCP1A
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
脱帽酶1A抗体产品介绍 background:
Cleavage of the 5＇-cap structure is involved in the major 5＇-to-3＇ ａnd nonsense-mediated mRNA decay pathways. The protein complex consisting of Dcp1 ａnd Dcp2 has been identified as the species responsible for the decapping reaction in Saccharomyces cerevisiae. In nonsense-mediated decay, the human decapping complex, made up of S. cerevisiae homologs hDcp1a ａnd hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. hDcp2 specifically hydrolyzes methylated capped RNA to release m(7)GDP, thereby aiding in mRNA degradation. Both hDcp1a ａnd hDcp2 colocalize in the cylasm. In addition, hDcp1a interacts with Smad4 forming a complex with TGF Beta ａnd BMP-4. hDcp1a ａnd Smad4 interact directly through a EVH1/WH1 domain on hDcp1a ａnd a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of hDcp1a as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of hDcp1a eliminates TGF Beta-induced nuclear translocation of hDcp1a.
Necessary for the degradation of mRNAs, both in normal mRNA turnover ａnd in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5＇-phosphorylated mRNA fragment ａnd 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.
Forms a complex with EDC3, DCP2, DDX6 ａnd EDC4/HEDLS, within this complex directly interacts with EDC3. Binds DCP1B, UPF1 ａnd SMAD4. Part of a cylasmic complex containing proteins involved in mRNA decay, including XRN1 ａnd LSM1. Interacts with PNRC2. Interacts with DDX17 in an RNA-independent manner. Interacts with ZC3HAV1.
Cylasm, P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies. Predominantly cylasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.
Tissue Specificity:
Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney ａnd pancreas.
Similarity:
Belongs to the DCP1 family.
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