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三磷酸腺苷依赖解旋酶DDX23抗体说明书
点击次数： 57发布时间：2021/9/22 14:41:59
- 上 传 者： 上海古朵生物科技有限公司
- 上传时间： 2021/9/22 14:41:59
- 文件大小： 17KB
- 文件类型： docx
- 所属分类： 操作维修手册
- 下载次数： 6次
- 查看次数： 57
资料简介： 英文名称 DDX23 三磷酸腺苷依赖解旋酶DDX23抗体产品介绍 background: Function: Subunit: Subcellular Location: Post-translational modifications: Similarity:
中文名称 三磷酸腺苷依赖解旋酶DDX23抗体
别 名 100 kDa U5 snRNP-specific protein; DEAD box protein 23; EC 3.6.1.-; PRP28 homolog; U5-100kD; DDX23_HUMAN.
规 格 0.1ml 0.2ml
研究域 细胞生物 细胞分化 表观遗传学
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 三磷酸腺苷依赖解旋酶DDX23抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 96kDa
细胞定位 细胞核
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human DDX23
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
DDX23 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear ａnd mitochondrial splicing, ａnd ribosome ａnd spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, ａnd cellular growth ａnd division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined.
Involved in pre-mRNA splicing ａnd its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation.
The phosphorylated form (by SRPK2) associates wth tri-snRNP (U4/U6-U5 tri-small nuclear ribonucleoproteins). Identified in the spliceosome C complex. Interacts with ERBB4.
Nuclear
In vitro phosphorylated by CLK1 ａnd U1 snRNP-associated protein kinase. Phosphorylated by SRPK2 ａnd this phosphorylation is required for its association with the tri-snRNP (U4/U6-U5 tri-small nuclear ribonucleoproteins) ａnd subsequent spliceosomal B complex formation. [SIMILARITY] Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
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