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DTNBP1抗原；异连蛋白结合蛋白1(DTNBP1)重组蛋白多种属
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详细内容
Recombinant Dystrobrevin Binding Protein 1 (DTNBP1)
异连蛋白结合蛋白1(DTNBP1)重组蛋白
[ PROPERTIES ]
Article Number:XY91635RPHu01
Specifications and prices: 50ug 1800.00 100ug 2880.00
Source: Prokaryotic expression
Host: E.coli
Residues: Gln100~Ser351
Tags: N-terminal His Tag
Tissue Specificity: Placenta, Brain, Lung.
Subcellular Location: Cell Junction, cell Membrane, cytoplasmic Vesicle, endoplasmic Reticulum, endosome, membrane, nucleus.
Purity: > 93%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Original Concentration: 200ug/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 4.3
Predicted Molecular Mass: 31.9kDa
Accurate Molecular Mass: 40kDa as determined by SDS-PAGE reducingconditions.
Phenomenon explanation:
The possible reasons that the actual band size differs fromthe predictedareasfollows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8°Cfor one month.
Aliquot and store at -80°Cfor 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
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热门标签：DBND,,HPS7,My031,Hermansky-Pudlak,syndrome,7,protein
