MSMb抗原；微精蛋白β(MSMb)重组蛋白多种属
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详细内容
Recombinant Microseminoprotein Beta (MSMb)
微精蛋白β(MSMb)重组蛋白
[ PROPERTIES ]
Article Number:XY93210RPHu01
Specifications and prices: 50ug 1800.00 100ug 2880.00
Residues: Asn19~Ile114,with two N-terminal Tags, His-tag and S-tag.
Host:E. coli
Subcellular Location: Secreted
Purity: >95%
Endotoxin Level: <1.0EU per 1µg
(determined by the LAL method).
Formulation:Supplied as lyophilized form in PBS,pH7.4, containing 1mM DTT, 5% trehalose, 0.01%sarcosyl and preservative.
Predicted isoelectric point: 5.6
Predicted Molecular Mass: 16.7kDa
Accurate Molecular Mass: 22kDa as determined by SDS-PAGE reducingconditions.
Applications:SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins fromthe samegene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8°Cfor one month.
Aliquot and store at -80°Cfor 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
The target protein is fused with two N-terminal Tags, His-tag andS-tag, itssequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGSEF- NASCYFIPNEGV PGDSTRKCMD LKGNKHPINS EWQTDNCETC TCYETEISCC TLVSTPVGYDKDNCQRIFKK EDCKYIVVEK KDPKKTCSVS EWII
[ IDENTIFICATION ]
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热门标签：MSM-B,,IGBF,MSP,,MSPB,Prostate,secreted,seminal,plasma,protein,,plasma,beta-inhibin
